Purification and characterization of cystatin from duck egg white

• Autorzy: Warwas M. , Gburek J. , Osada J. , Golab K.
• Języki publikacji: EN

Abstrakty

EN

It is the second peptidase inhibitor, after ovostatin, which showing the same antipapain activity in egg white in different avian species implies differences in amino -acid sequences. Cystatin from duck egg white was purified by carboxymethylpapain affinity chromatography and size-exclusion HPLC. The purified inhibitor which showed partial identity in the immunodiffusion test with chicken egg white cystatin, had an apparent molecular mass of 9.3 kDa as determined by SDS/PAGE. IEF analysis revealed five molecular forms of pi in the range 7.8-S.4. The obtained cystatin was neither glycosylated nor phosphorylated as it is in the case of chicken cystatin. The determined Ki (0.005 ± 0.001 nM) was similar to that reported for human and chicken cystatin C.

Słowa kluczowe

EN

protein; duck egg; chicken; cystatin; egg; peptidase inhibitor

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 3
• Opis fizyczny: p.351-356,fig.

Twórcy

autor

Warwas M.

• Medical Academy, Szewska 38-39, 50-139 Wroclaw, Poland

autor

Gburek J.

autor

Osada J.

autor

Golab K.

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