Effect of BAP, TDZ and CPPU on multiple shoot formation in pea [Pisum sativum L.] in culture in vitro

• Autorzy: Tulac S. , Leljak-Levanic D. , Krsnik-Rasol M. , Jelaska S.
• Języki publikacji: EN

Abstrakty

EN

Shoot regeneration in five pea (Pisum sativum L.) cultivars (Atlas, Avola, Karina, Mali provansalac and Tristar) was achieved by direct culture of mature seeds on MSB5 medium supplemented with either N6-benzylaminopurine (BAP), N-phenyl-N’(-l,2,3-thiadiazol-5-yl)urea (thidiazuron, TDZ) or N-(2-chloro-4-pyridyl)-N’-phenylurea (forchlorfenuron, CPPU). Multiple shoots formed de novo without an intermediary callus phase at the cotyledonary notch, in the axillary meristem regions of the seedlings, and in the hypocotyl subepidermal tissues within two to three weeks of culture initiation. Bud formation began after 5 to 7 days of treatment and the number of buds increased with the duration of culture and increasing concentration of growth regulators. Transient exposure to plant growth regulators (24-28 h) was sufficient to induce bud formation. CPPU was the most effective and BAP the least effective for the induction of regeneration. Separated shoots (1-2 cm) were rooted (60%) on MSB5 medium supplemented with 1.1 µM indole-3- acetic acid (IAA) and 2.0 µM α-naphthaleneacetic acid (NAA) and developed into flowering plants.

Słowa kluczowe

EN

tissue culture; phenylurea derivative; Karina cultivar; regeneration; Atlas cultivar; pea; in vitro; pea plant; flowering plant; callus culture; 6-benzylaminopurine; Mali cultivar; Avola cultivar; tobacco; Tristar cultivar; shoot; cell division; Pisum sativum

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2002
• Tom: 44
• Opis fizyczny: p.161-168,fig.,ref.

Twórcy

autor

Tulac S.

• University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia

autor

Leljak-Levanic D.

autor

Krsnik-Rasol M.

autor

Jelaska S.

Bibliografia

• Bencheikh M, and Gallais A. 1996. Study of the variation in the somatic embryogenesis ability of some pea lines (Pisum sativum L. and Pisum arvense L.). Euphytica 90: 251-256.
• Böhmer P, Meyer B, and Jacobsen HJ. 1995. Thidiazuron-induced high frequency of shoot induction and plant regeneration in protoplast-derived pea callus. Plant Cell Reports 15: 26-29.
• Buising CM, Shoemaker RC, and Benbow RM. 1994. Early events of multiple bud formation and shoot development in soybean embryonic axes treated with the cytokinin, 6-benzylaminopurine. American Journal of Botany 81: 1435-1448.
• Gamborg OL, Miller RA, and Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50: 151-158.
• Griga M, Tejklowa E, Novak FJ, and Kubalankowa M. 1986. In vitro clonal propagation of Pisum sativum L. Plant Cell, Tissue and Organ Culture 6: 95-104.
• Hayata Y, and Niimi Y. 1995. Synthetic cytokinin - l-(2-chloro-4-pyridyl)-3-phenylurea (CPPU) - promotes fruit set and induces parthenocarpy in watermelon. Journal of the American Society for Horticultural Science 120: 996-1000.
• Hussey G, and Gunn HV. 1984. Plant production in pea (Pisum sativum L. cvs. Puget and Upton) from long-term callus with superficial meristems. Plant Science Letters 37: 143-148.
• Jarkova TV, Deineko EV, and Shumny VK. 1998. Screening of pea (Pisum sativum L.) collection effects on the morphogenetic capacity in tissue culture. Plant Tissue Culture and Biotechnology 4: 175-182.
• Kysely W, and Jacobsen HJ. 1990. Somatic embryogenesis from pea embryos and shoot apices. Plant Cell, Tissue and Organ Culture 20: 7-14.
• Kysely W, Myers JR, Lazzeri PA, Collins GB, and Jacobsen HJ. 1987. Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.). Plant Cell Reports 11: 305-308.
• Lehminger-Mertens R, and Jacobsen HJ. 1989. Plant regeneration from pea protoplasts via somatic embryogenesis. Plant Cell Reports 8: 379-382.
• Malik KA, and Saxena PK. 1991. Regeneration in Phaseolus vulgaris L. Promotive role of N6-benzylaminopurine in cultures from juvenile leaves. Planta 184: 148-150.
• Malik KA, and Saxena PK. 1992a. Regeneration in Phaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron. Planta 186: 384-389.
• MalikKA, and Saxena PK. 1992b. In vitro regeneration of plants: A novel approach. Naturwissenschaften 79: 136-137.
• Malik KA, and Saxena PK. 1992c. Thidiazuron induces high-frequency shoot regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris). Australian Journal of Plant Physiology 19: 731-740.
• Mok MC, Mok DWS, Turner JE, and Mujer CV. 1987. Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems. HortScience 22/6: 1194- 1197.
• Mok MC, Mok DWS, Amstrong DJ, Shudo K, Isogai Y, and Okamoto T. 1982. Cytokinin activity of N-phenyl-N, (-1,2,3- thidiazol-5-yl)urea (thidiazuron). Phytochemistry 21: 1509- 1511.
• Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
• Murthy BNS, and Saxena PK. 1994. Somatic embryogenesis in peanut (Arachis hypogaea L.): Stimulation of direct differentiation of somatic embryos by forchlorfenuron (CPPU). Plant Cell Reports 14: 145-150.
• Murthy BNS, Murch SJ, and Saxena PK. 1995. Thidiazuron-induced embryogenesis in intact seedlings of peanut (Arachis hypogaea L.): Endogenous growth regular levels and significance of cotyledons. Physiologia Plantarum 94: 268-276.
• Murthy BNS, Victor J, Singh RP, Fletcher RA, and Saxena PK. 1996. In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron. Plant Growth Regulation 19: 133-140.
• Nadolska-Orczyk A, Milkowska L, and Orczyk W. 1994. Two ways of plant regeneration from immature cotyledons of pea. Acta Societatis Botanicorum Poloniae 63: 153-157.
• Nawerby B, Madsen M, Chrystiansen J, and Wyndaeler R. 1991. A rapid and efficient regeneration system for pea (Pisum sativum), suitable for transformation. Plant Cell Reports 9: 676-679.
• Nielsen SVS, Poulsen GB, and Larsen ME. 1991. Regeneration of shoots from pea (Pisum sativum) hypocotyl explants. Physiologia Plantarum 82: 99-102.
• Okamoto T, Shudo K, Takahashi S, Tatsunami IT, Isogai Y, and Yamada K. 1978. Shoot formation of tobacco callus by various cytokinin-active ureas and pyrimidines. Chemical and Pharmaceutical Bulletin (Tokyo) 26: 3250-3252.
• Ozcan S, Barghchi M, Firek S, and Draper J. 1992. High frequency adventitious shoot regeneration from immature cotyledons of pea (Pisum sativum L.). Plant Cell Reports 11: 44-47.
• Ozcan S, Barghchi M, Firek S, and Draper J. 1993. Efficient adventitious shoot regeneration and somatic embryogenesis in pea. Plant Cell, Tissue and Organ Culture 34: 271-277.
• Puonti-Kaerlas J, and Eriksson T. 1988. Improved protoplast culture and regeneration of shoots in pea (Pisum sativum L.). Plant Cell Reports 7: 242-245.
• Reynolds JF. 1987. Chemical regulation in tissue culture: an overview. HortScience 22/6: 1192-1193.
• Saxena PK, Malik AK, and Gill R. 1992. Induction by thidiazuron of somatic embryogenesis in intact seedlings of peanut. Planta 187: 421-424.
• Takahashi SK, Shudo K, Okamoto T, Yamada K, and Isogai Y. 1978. Cytokinin activity of N-phenyl-N’-(4-pyridyl)urea derivatives. Phytochemistry 17: 1201-1207.
• Tegeder M, Gebhardt D, Schieder O, and Pickardt T. 1995. Thidiazuron-induced plant regeneration from protoplasts of Vicia faba cv. Mythos. Plant Cell Reports 15: 164-169.
• Tétu T, Sangwan RS, and Sangwan-Norreel BS. 1990. Direct somatic embryogenesis and organogenesis in cultured immature zygotic embryos of Pisum sativum L. Journal of Plant Physiology 137: 102-109.
• Thomas JC, and Katterman FR. 1986. Cytokinin activity induced by thidiazuron. Plant Physiology 81: 681-683.