Rabbit haemorrhagic disease virus: studies on field strains isolated in Poland

• Autorzy: Chrobocinska M
• Języki publikacji: EN

Abstrakty

EN

The standard strain and 78 field samples of rabbit haemorrhagic disease were tested by the haemagglutination test (HA) and ELISA. The occurrence of 17% false negative results in HA test was demonstrated. The results of studies of 30 out of 78 samples examined by the ELISA prepared in our laboratory in comparison with commercial kit are presented. The high correlation of results obtained in ELISA kits was observed. The structural proteins of 17 viral sarnples were analysed by the SDS-Page and Western blot methods. HA positive samples demonstrated the presence of major structural protein with molecular weight of about 60 kD and in some samples the protein with m.w. of 38 kD. In HA false negative samples the proteins of about 60 and 26-28 kD were demonstrated. The viral proteins recognized by positive rabbit homologic and hare heterologic sera, did not react with negative rabbit serum.

Słowa kluczowe

EN

negative rabbit serum; virus; clinical symptom; Poland; hemagglutination test; ELISA test; rabbit hemorrhagic disease; structural protein

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1996
• Tom: 40
• Numer: 2
• Opis fizyczny: p.61-67,fig.

Twórcy

autor

Chrobocinska M

• National Veterinary Research Institute, 24-100 Pulawy, Poland

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