Molecular characterization of the genomic region harboring the BYDV-resistance gene Bdv2 in wheat

• Autorzy: Gao L , Ma Q , Liu Y , Xin Z , Zhang Z
• Języki publikacji: EN

Abstrakty

EN

Barley yellow dwarf virus (BYDV) can cause significant losses of wheat worldwide. The long arm segment of Thinopyrum intermedium chromosome 7Ai#1 carrying the BYDV resistance gene Bdv2 was translocated to the distal region of the long arm of wheat chromosome 7D in translocation line Yw642. In this study, 40 wheat EST sequences located in the distal region of 7DL were explored to identify specific PCR markers for the Bdv2 region on the basis of the homoeologous relationship between wheat chromosome 7D and Th.intermedium chromosome 7Ai#1. Our results revealed 8 novel EST-PCR markers specific to the Bdv2 region, including 5 EST-STS markers of BE404744, BE498985, BE591497, BG606695 and BQ161842, and 3 EST-SSCP markers of BE404953, BG312663 and BE498985. These EST-PCR markers could distinguish Bdv2 from another BYDV-resistance gene located on Th.intermedium chromosome 2Ai-2. These specific bands for the Bdv2 region were further cloned and sequenced. The sequencing analysis indicated that the specific sequences for the Bdv2 region were highly homologous with the original wheat EST sequences that were used to design primers, and encode respectively a protein kinase, P450, centrin, transducin, and a hypothetical protein. This study created a starting point for eventual cloning of the Bdv2 gene and understanding the defense mechanism.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2009
• Tom: 50
• Numer: 2
• Opis fizyczny: p.89-98,fig.,ref.

Twórcy

autor

Gao L

• National Key Science Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
• Inner Mongolia Agricultural University, Hohhot, China

autor

Ma Q

• Inner Mongolia Agricultural University, Hohhot, China

autor

Liu Y

• State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China

autor

Xin Z

• National Key Science Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

autor

Zhang Z

• National Key Science Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Bibliografia

• Ayala L, Henry M, Gonz N, Ginkel M, Mujeeb-Kazi A, Keller B, Khairallah M, 2001 a. A diagnostic molecular marker allowing the study of Th. intermedium-derived resistance to BYDV in bread wheat segregating populations. Theor Appl Genet 102: 942-949.
• Ayala L, Ginkel M, Khairallah M, Keller B, Henry M, 2001b. Expression of Thinopyrum intermedium-derived barley yellow dwarf virus resistance in elite bread wheat backgrounds. Phytopathology 91: 55-62.
• Ayala LN, Bariana HS, Singh RP, Gibson JM, Gibson AA, Mechanicos PJ, 2007. Trigenomic chromosomes by recombination of Thinopyrum intermedium and Th. ponticum translocations in wheat. Theor Appl Genet 116: 63-75.
• Banks PM, Larkin PJ, Bariana HS, Lagudah ES, Appels R, Waterhouse PM, et al. 1995. The use of cell culture for subchromosomal introgressions of barley yellow dwarf virus resistance from Thinopyrum intermedium to wheat. Genome 38: 395-405.
• Bassam BJ, Anollés CG, Gresshoff MP, 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry 196: 80-83.
• Brettell RIS, Banks PM, Cauderon Y, Chen X, Cheng ZM, Larkin PJ, Waterhouse PM, 1988. A single wheatgrass chromosome reduces the concentration of barley yellow dwarf virus in wheat. Ann Appl Biol 113:599-603.
• Fedak G, Chen Q, Conner R, Laroche A, Comeau A, ST.-Pierre C, 2001. Characterization of wheat-Thinopyrum partial amphiploids for resistance to barley yellow dwarf virus. Euphytica 120: 373-378.
• Friebe B, Mukai Y, Gill BS, Cauderon Y, 1992. C-banding and in situ hybridization analysis of Agropyron intermedium, a partial wheat-Agropyron intermedium amphiploid, and six derived chromosome addition lines. Theor Appl Genet 84: 899-905.
• Cauderon Y, Saigne B, Dauge M, 1973. The resistance to wheat rusts of Agropyron intermedium and its use in wheat improvement. In: Sears ER, Sears LMS, eds. Proc 4th Int Wheat Genet Symp Wheat Improv. University of Missouri, Columbia, Mo.: 401-407.
• Ginkel MV, Henry M, 2002. Breeding for BYDV tolerance/resistance in CIMMYT bread wheats targeted to developing countries, barley yellow dwarf disease. In: Henry M, McNab A, eds. Barley yellow dwarf disease: Recent advances and future strategies. CIMMYT, Mexico, D.F. Mexico: 34-39.
• Hohmann U, Badaeva K, Busch W, Badaeva K, Friebe B, Gill B, 1996. Molecular cytogenetic analysis of Agropyron chromatin specifying resistance to barley yellow dwarf virus in wheat. Genome 39: 336-347.
• Larkin PJ, Baeva K, Banks PM, Lagudah ES, Appels R, Chen X, Xin ZY, Ohm HW, McIntosh RA, 1995. Disomie Thinopyrum intermedium addition lines in wheat with barley yellow dwarf virus resistance and with rust resistances. Genome 38: 385-394.
• Larkin P, Kleven S, Banks P, 2002. Utilizing Bdv2, the Thinopyrum intermedium source of BYDV resistance, to develop wheat cultivars. In: Henry M, Mcnab A, eds. Barley yellow dwarf disease: Recent advances and future strategies. CIMMYT, Mexico, D.F. Mexico: 60-63.
• Lin ZS, Cui ZF, Zeng XY, Ma YZ, Zhang ZY, Nakamura T, et al. 2007. Analysis of wheat-Thinopyrum intermedium derivatives with BYDV-resistance. Euphytica 158: 109-118.
• McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ, 2001. Catalog of gene symbols for wheat: Supplement pp 14. http://wheat.pw.usda.gov/ggpages/wgc/2001upd.html.
• Michelmore RW, Paran I, Kesseli RV, 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic region using segregation populations. Proc Natl Sci USA 88: 9828-9832.
• Pan G, Zhang X, Liu K, Zhang J, Wu X, Zhu J, Tu J, 2006. Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides. Plant Mol Biol. 61: 933-943.
• Qi LL, Echalier B, Chao S, Lazo GR, Butler GE, Anderson OD, et al. 2004. A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploidy wheat. Genetics 168: 701-712.
• Qian YT, Zhou GH, 1986. Studies on screening technique for wheat yellow dwarf virus in the field. Acta Phytophylacica Sin. 13: 159-164. (In Chinese with English abstract).
• Sharma H, Ohm H, Goulard L, Lister R, Appels R, Benlhabib O, 1995. Introgression and characterization of barley yellow dwarf virus resistance from Thinopyrum intermedium into wheat. Genome 38: 406-413.
• Sharp PG, Kreis M, Shewry PR, Gale MD, 1988. Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet 75: 289-290.
• Singh R, Burnett P, Albarran M, Sanjaya R, 1993. Bdv1: a gene for tolerance to barley yellow dwarf virus in bread wheats. Crop Sci 33: 231-234.
• Stoutjesdijk P, Kammholz S, Kleven S, Matsay S, Banks PM, Larkin PJ, 2001. PCR-based molecular marker for the Bdv2 Thinopyrum intermedium source of barley yellow dwarf virus resistance in wheat. Aust J Agric Res 52: 1383-1388.
• Xin ZY, Xu HJ, Chen X, Lin ZS, Zhou GH, Qian YT, et al. 1991. Development of common wheat germplasm resistant to barley yellow dwarf virus by biotechnology. Sci China Ser B. 34: 1055-1062.
• Xin ZY, Zhang ZY, Chen X, Lin ZS, Ma Y, Xu HJ, et al. 2001. Development and characterization of common wheat -Thinopyrum intermedium translocation lines with resistance to barley yellow dwarf virus. Euphytica 119: 161—165.
• Zhang MY, Xin ZY, Cao YP, Xu hj, Ning DX, Chen X, 2005. An elite winter wheat variety Lingkang 11 with multi-resistance and its cultivation skill. J Triticeae Crops 25: 153 (In Chinese).
• Zhang ZY, Xin ZY, Ma YZ, Chen X, Xu QF, Lin ZS, 1999. Mapping of a BYDV resistance gene from Thinopyrum intermedium in wheat background by molecular markers. Sci China Ser C 42: 663-668.
• Zhang ZY, Xin ZY, Chen X, Qian YT, Lin ZS, Ma YZ, 2000. Molecular cytogenetic characterization of a new wheat line Yw243 with resistance to barley yellow dwarf virus. Acta Genet Sin 27: 614-620. (in Chinese with English abstract).
• Zhang ZY, Xin ZY, Larkin P, 2001. Molecular characterization of a Thinopyrum intermedium group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus. Genome 44: 1129-1135.
• Zhang ZY, Xu JS, Xu QJ, Larkin P, Xin ZY, 2004. Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Theor Appl Genet 109: 433-439.
• Zhou G, Zhang S, Qian Y, 1986. Identification and application of four strains of wheat yellow dwarf virus. Scientia Agric Sin 20: 7-12 (In Chinese).
• Zhou J, Tang X, Martin G, 1997. The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes. EMBO J. 16: 3207-3218.