Wplyw agonisty receptorow adrenegicznych beta2-clenbuterolu na mineralizacje kosci piszczelowej szczura w warunkach ustalonej osteopenii

• Autorzy: Radzki R P , Bienko M. , Kapica M. , Puzio I. , Dobrowolski P. , Filip R.

Warianty tytułu

EN

Effect of beta2-adrenergic receptor agonist clenbuterol on the mineralization of tibiae in rats with established osteopenia

• Języki publikacji: PL

Abstrakty

EN

The purpose of the study was to determine the effect β₂-adrenergic receptor agonist-clenbuterol on tibiae mineralization of female rats with established osteopenia. The experiments were conducted on 30 female 3-months-of-age Wistar rats with an initial body weight of about 250 g. The sham-operation - SHO (n=10) and bilateral ovariectomy - OVX (n=20) were performed. After 60 days of osteopenia induction the ovariectomized rats were divided into a group fed a standard diet (n=10) and a group that received a diet supplied with clenbuterol at a dose of 5 mg/kg. After 14 days of the experiment the tibiae was isolated and tested using a DEXA densitometer, peripheral quantitative computer tomography (pQCT) and three-point bending test. The obtained results proved that a 14 day period of clenbuterol treatment significantly increased mechanical properties content and mineral density, both planar (BMD) and volumetric (vTotBMD) of the tibiae of ovariectomized rats.

Słowa kluczowe

PL

szczury; clenbuterol; osteopenia; mineralizacja; kosc piszczelowa

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 07
• Opis fizyczny: s.824-826,tab.,bibliogr.

Twórcy

autor

Radzki R P

• Akademia Rolnicza, ul.Akademicka 12, 20-033 Lublin

autor

Bienko M.

autor

Kapica M.

autor

Puzio I.

autor

Dobrowolski P.

autor

Filip R.

Bibliografia

• 1. Agrawal S., Thakur P., Katoch S. S.: Beta adrenoceptor agonists, clenbuterol, and isoproterenol retard denervation atrophy in rat gastrocnemius muscle: use of 3-methylhistidine as a marker of myofibrillar degeneration. Jpn. J. Physiol. 2003, 53, 229-237.
• 2. Beckett A. H.: Clenbuterol and sport. Lancet 1992, 340, 1165-
• 3. Boyd D., O'Keeffe M., Smyth M. R.: Matrix solid-phase dispersion as a multiresidue extraction technique for beta-agonists in bovine liver tissue. Analyst. 1994, 119, 1467-1470.
• 4. Brambilla G., Civitareale C., Pierdominici E., De Giovanni F., Anastasio A.: Synovial fluid as a matrix of selection in the detection of beta-adrenergic agonist drugs in carcases and fresh meat. Analyst. 1994, 119, 2591-2593.
• 5. Compston J. E.: Sex steroids and bone. Physiol Rev. 2001, 81, 419-447.
• 6. Dumestre-Toulet V., Cirimele V., Ludes B., Gromb S., Kintz P.: Hair analysis of seven bodybuilders for anabolic steroids, ephedrine, and clenbuterol. J. Forensic Sci. 2002, 47, 211-214.
• 7. Duncan N. D., Williams D. A., Lynch G. S.: Deleterious effects of chronic clenbuterol treatment on endurance and sprint exercise performance in rats. Clin. Sci. (Lond). 2000, 98, 339-347.
• 8. Ferretti J. L., Capozza R. F., Zanchetta J. R.: Mechanical validation of a tomographic (pQCT) index for noninvasive estimation of rat femur bending strength. Bone 1996, 18, 97-102.
• 9. Kazanietz M. G., Enero M. A.: Role of cyclic AMP in the release of noradrenaline from isolated rat atria. Effect of pretreatment with clenbuterol. Naunyn Schmiedebergs Arch Pharmacol. 1992, 346, 311-314.
• 10. Kellenberger S., Muller K., Richener H., Bilbe G.: Formoterol and isoproterenol induce c-fos gene expression in osteoblast-like cells by activating beta2-adrenergic receptors. Bone 1998, 22, 471-478.
• 11. Macdonald H. M., Kontulainen S. A., kelvie-O'brien K. J., Petit M. A., Janssen P., Khan K. M., McKay H. A.: Maturity- and sex-related changes in tibial bone geometry, strength and bone-muscle strength indices during growth: A 20-month pQCT study. Bone 2005,
• 12. Mazzanti G., Daniele C., Boatto G., Manca G., Brambilla G., Loizzo A.: New beta-adrenergic agonists used illicitly as growth promoters in animal breeding: chemical and pharmacodynamic studies. Toxicology 2003, 187, 91-99.
• 13. Meinel L., Zoidis E., Zapf J., Hassa P., Hottiger M. O., Auer J. A., Schneider R., Gander B., Luginbuehl V., Bettschart-Wolfisberger R., Illi O. E., Merkle H. P., von R. B.: Localized insulin-like growth factor I delivery to enhance new bone formation. Bone 2003, 33, 660-672.
• 14. Mundy G. R., Martin T. J.: Physiology and pharmacology of bone. Springer-Verlag, Berlin 1993.
• 15. Navegantes L. C., Resano N. M., Baviera A. M., Migliorini R. H., Kettelhut I. C.: Effect of sympathetic denervation on the rate of protein synthesis in rat skeletal muscle. Am. J. Physiol Endocrinol. Metab. 2004, 286, E642-E647.
• 16. Ordway G. A., O'Donnell J. M., Frazer A.: Effects of clenbuterol on central beta-1 and beta-2 adrenergic receptors of the rat. J. Pharmacol. Exp. Ther. 1987, 241, 187-195.
• 17. Papakitsou E. F., Margioris A. N., Dretakis K. E., Trovas G., Zoras U., Lyritis G., Dretakis E. K., Stergiopoulos K.: Body mass index (BMI) and parameters of bone formation and resorption in postmenopausal women. Maturitas. 2004, 47, 185-193.
• 18. Radzki R. P., Bieńko M., Puzio I., Studziński T.: Wpływ estradiolu i tamoksifenu na właściwości fizyczne kości udowej i ramiennej kurcząt brojlerów. Medycyna Wet. 2003, 59, 1090-1094.
• 19. Sander K., Deegen E., Ohnesorge B.: Influencing respiratory gas analytical lung function parameters by intravenous injection of clenbuterol in horses with chronic lung diseases. Dtsch. Tierärztl. Wochenschr. 2002, 109, 295-299.
• 20. Schneider P., Reiners C., Cointry G. R., Capozza R. F., Ferretti J. L.: Bone quality parameters of the distal radius as assessed by pQCT in normal and fractured women. Osteoporos. Int. 2001, 12, 639-646.
• 21. Schoppen S., Carbajal A., Perez-Granados A. M., Vivas F., Vaquero M. P.: Food, energy and macronutrient intake of postmenopausal women from a menopause program. Nutr. Hosp. 2005, 20, 101-109.
• 22. Suzuki A., Palmer G., Bonjour J. P., Caverzasio J.: Catecholamines stimulate the proliferation and alkaline phosphatase activity of MC3T3-E1 osteoblast-like cells. Bone 1998, 23, 197-203.
• 23. Young O. A., Watkins S., Oldham J. M., Bass J. J.: The role of insulin-like growth factor I in clenbuterol-stimulated growth in growing lambs. J. Anim. Sci. 1995, 73, 3069-3077.