Association of the polymorphism at defensin gene loci with dairy production traits and milk somatic cell count in Black-and-White cows

• Autorzy: Ryniewicz Z , Zwierzchowski L. , Bagnicka E. , Flisikowski K. , Maj A. , Krzyzewski J. , Strzalkowska N.
• Języki publikacji: EN

Abstrakty

EN

Because of the antimicrobial role that defensins play in humans and animals, genes encoding these peptides may be considered as molecular markers of a genetically determined susceptibility (or resistance) of the mammary gland to mastitis. Records were gathered of daily milk yield, fat, protein, and lactose content of milk, and milk somatic cell count (SCC) of 217 lactating Black-and-White cows. To determine the defensin gene polymorphism, DNA was isolated from blood and the RFLP method with enzyme TaqI was used. Twenty different polymorphic systems were revealed, possibly representing variants of genes encoding different defensins. Statistical evaluation included cows with more than seven records, and showing the 2.5% frequency of combined defensin genotypes (CDGs). In this way 13 different CDGs of 204 cows appeared available for statistical evaluation. CDGs significantly affected all dairy performance traits studied, as well as SCC. The important message from these results is that the defensin(s) may probably be used as genetic marker(s) in the breeding programmes aiming at selecting highly productive dairy cattle with increased resistance to udder infections.

PL

Ze względu na fakt, że defensyny pełnią istotną rolę w obronie organizmów przed zakażeniami bakteryjnymi, geny kodujące te peptydy mogłyby stać się dobrymi markerami genetycznej wrażliwości lub odporności gruczołu mlekowego na stany zapalne wywoływane przez drobnoustroje (mastitis). Wykorzystano dane o dziennej wydajności, procencie tłuszczu, białka i laktozy w mleku oraz o liczbie komórek somatycznych mleka 217 krów dojnych. Z krwi krów izolowano DNA, a następnie metodą RFLP, używając enzymu TaqI, określono polimorfizm genów defensyn. Uzyskano 20 polimorficznych układów (combined defensin genotypes – CDG), reprezentujących warianty genów różnych defenzyn. Do analizy statystycznej użyto danych, pochodzących od krów, których mleko badano co najmniej osiem razy i o układach genotypów o częstości większej niż 2,5%. W rezultacie analizę przeprowadzono dla 13 różnych układów genotypów, stwierdzonych u 204 krów. Wykazano wysoce istotny wpływ układów genów defensyn (CDG) na wszystkie badane cechy produkcyjne i liczbę komórek somatycznych (SCC) w mleku. Wnioskuje się,że uzyskane wyniki dają nadzieję na wykorzystanie w selekcji bydła mlecznego polimorfizmu genów defensyn jako markera wysokiej produkcyjności i zwiększonej odporności krów na bakteryjne zakażenia wymienia. Konieczne są jednak dalsze badania.

Słowa kluczowe

EN

Black-and-White breed; cattle; dairy cattle; cow; dairy cow; gene polymorphism; defensin; coding gene; genetic marker; antimicrobial peptide; milk; milk trait; milk analysis; daily performance; dairy production; somatic cell number; udder; genetic resistance; cattle breeding

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2003
• Tom: 21
• Numer: 4
• Opis fizyczny: p.209-222,fig.,ref.

Twórcy

autor

Ryniewicz Z

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Zwierzchowski L.

autor

Bagnicka E.

autor

Flisikowski K.

autor

Maj A.

autor

Krzyzewski J.

autor

Strzalkowska N.

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