Plant regeneration from Onobrychis subnitens Bornm. hypocotyl explants via somatic embryogenesis and organogenesis

• Autorzy: Karamian R , Ranjbar M.
• Języki publikacji: EN

Abstrakty

EN

An efficient procedure is established here for plant regeneration from hypocotyl explants in Onobrychis subnitens Bornm., an important forage legume in Iran. Two types of calli, embryogenic and non-embryogenic, were induced from hypocotyl explants on MS medium supplemented with 2,4-D and BAP at different concentrations. To initiate morphogenesis, embryogenic calli were transferred to MS medium with 0.5 mg·l-1 NAA and 1 mg·l-1 BAP. Initiated somatic embryos or adventitious buds developed into plantlets following culture on MS medium without any growth regulators or with 2 mg·l-1 NAA, respectively. All the regenerated plants were normal with respect to morphology and growth characters.

Słowa kluczowe

PL

embryogenesis; organogenesis; hypocotyl explant; plant regeneration; hypocotyl culture; Onobrychis subnitens; somatic embryogenesis; Iran; morphology; growth

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2008
• Tom: 50
• Numer: 2
• Opis fizyczny: p.13-18,fig.,ref.

Twórcy

autor

Karamian R

• Department of Biology, Bu Ali Sina University, P.O.Box 65175/4161, Hamedan, Iran

autor

Ranjbar M.

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