Wplyw podawania cyclofosfamidu na jakosc nasienia psow

• Autorzy: Brodzki P , Wrona Z. , Krakowski L. , Brodzki A. , Lopuszynski W.

Warianty tytułu

EN

Influence of administration of cyclophosphamide on the quality of male dogs semen

• Języki publikacji: PL

Abstrakty

EN

The aim of the research was to determine the influence of cyclophosphamide administered in cancer treatment to dogs on the chosen parameters of the semen quality. The research was carried out on 12 male dogs of mixed breed, from 5- to 10-years-of-age, with body weight between 15 and 36 kg. The animals were in good physical condition and with proper sex drive, which was an indispensable condition for the experiment. The animals were divided into two groups. The experimental group consisted of 6 dogs, which after having the tumor removed surgically, were administered cyclophosphamide by mouth in the form of tablets, in doses of 50 mg/m2 per body surface for 21 days. The control group consisted of 6 clinically healthy dogs. The animals from both groups were subjected to the same research procedure which involved daily clinical examination, with particular attention paid to the functioning of the genital system, and complete semen estimation. The above mentioned tests were performed twice at two-week intervals before administering the medication, three times at seven-day intervals during the treatment and twice at two-week intervals after the application of the drug was discontinued. Moreover, histopathological examination of testicles was additionally performed in randomly chosen representatives of each group during the treatment (on 12th day) and about 50 days after the treatment was discontinued. Each group of animals was studied and observed for, at least, 10 weeks. As the result of cyclophosphamide treatment, there was an increase in the percentage of dead spermatozoa, decreased spermatozoon concentration, a drop in the number of spermatozoa with correct functions of the cell membrane, a rise in the percentage of spermatozoa with primary and secondary abnormalities (defects). Histopatological examination confirmed slight damage to spermatogenic epithelium that resulted in a deterioration of the semen quality.

Słowa kluczowe

PL

samce; psy; cyclofosfamid; spermatogeneza; chemioterapia; jakosc; rozrod zwierzat; nasienie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 04
• Opis fizyczny: s.439-443,fot.,tab.bibliogr.

Twórcy

autor

Brodzki P

• Uniwersytet Przyrodniczy w Lublinie, ul.Gleboka 30, 20-612 Lublin

autor

Wrona Z.

autor

Krakowski L.

autor

Brodzki A.

autor

Lopuszynski W.

Bibliografia

• 1.Aubrey D. A.: Massive hepatic necrosis after cyclophosphamide. Brit. Med. J. 1970, 3, 58. 2.Bielas W.: Wpływ różnych metod konserwacji nasienia knura w niskich temperaturach na wybrane właściwości plemników. Praca dokt., Wydz. Medycyny Weterynaryjnej AR, Wrocław 1999.
• 3.Blöm E.: Ocena morfologiczna wad plemników buhaja. II. Propozycja nowej klasyfikacji wad plemników. Medycyna Wet. 1981, 37, 239-242.
• 4.Bramswig J. H., Heimes U., Heiermann E., Schlegel W., Niesche E., Schellong G.: The effects of diffetent cumulative doses of chemotcherapy on testicular function. Results in 75 patients treated for Hodgkin's disease during childh or adolescence. Cancer 1990, 65, 1298-1302.
• 5.Buchman J. D., Forrley K. F., Barrie J. U.: Return of spermatogenesis after stopping cyclophosphamide therapy. Lancet 1975, 26, 156-157.
• 6.Chark B. S., Gupta R., Mandrekar P., Sheth N. A., Banavali S. D., Saikia- -Gopal R., Dinshaw K. A., Adrani S. H.: Testicular dysfunction after cyclophosphamide vincristine procarbazine prednisolone chemotherapy for advanced Hodgkin's disease. A long term follow-up study. Cancer 1990, 65, 1903-1906.
• 7.Demkow T.: Wpływ chemioterapii na płodnośæ chorych na nowotwór jądra. Urologia Pol. 2000, 53, 320-329.
• 8.Demkow T., Faundez R., Komoda J.: Ocena parametrów nasienia u chorych na raka jądra przed i po chemioterapii. Gin. Pol. 2002, 75, 10.
• 9.Dobrzyñska M. M., Lenarczyk M., Gajewski A. K.: Induction of dominant lethal mutations after exposure of male mice to cyclophosphamide. Roczn. Pañst. Zakł. Hig. 1998, 49, 285-291. 10.Dynarowicz I.: Chemioterapia chorób nowotworowych zwierząt. Onkologia Weterynaryjna. Postępy w diagnostyce i terapii. Mat. I Konf.: Onkologia weterynaryjna postępy w diagnostyce i terapii, Olsztyn 1997, s. 69-77.
• 11.England G. C. W.: Rozród i położnictwo psów według Allena. Wyd. SIMA WLW, Warszawa 1998.
• 12.England G. C. W., Palmer J. M.: Hypoosmotic swelling of dog spermatozoa. J. Reprod. Fert 1993, 47, 261-270.
• 13.Howell S. J., Radford J. A., Ryder W. D., Shalet S. M.: Testicular function after cytotoxic chemotherapy: evidence of Leydig cell insufficiency. J. Clin. Oncol. 1999, 17, 1493-1498.
• 14.Kaur F., Sangha G. K., Billospuri G. S.: Cyclophosphamide induced structural and biochemical changes in testis and epididymidis of rats. Indian J. Exp. Biol. 1977, 35, 771-775.
• 15.Kenney L. B., Laufer M. R., Grant F. D.,Grier H., Diller I.: High risk of infertility and long term gonadal damage in males treated with high dose cyclophosphamide for sarcoma during childhood. Cancer 2001, 91, 613-621.
• 16.Kieza Z.: Chemioterapia onkologiczna w wybranych schorzeniach nowotworowych u psów. Mat. I Konf. Onkologicznej. Wybrane zagadnienia z chorób nowotworowych psa i kota. Szczecin Maciejowo 17-18 maja 2003, s. 27-30.
• 17.Kowalska A.: Zaburzenia czynności gonad jako wynik działania leków przeciwnowotworowych diagnostyka, objawy, zapobieganie, leczenie. Pol. Tyg. lek. 1993, XLVIII, 572-575.
• 18.Lampe H., Horwich A., Norman A., Nicholls J., Dearnaley D. P.: Fertility after chemotherapy for testicular germ cell cancers. J. Clin. Oncol. 1997, 15, 239-245.
• 19.Lechniak D., Cumming J. R., Kaczmarek J.: Wykorzystanie testu oporności osmotycznej plemników w rutynowej ocenie jakości nasienia buhajów. Medycyna Wet. 1998, 54, 741-743.
• 20.Lechniak D., Kędzierski A., Stanisławski D.: The use of HOS test to evaluate membrane functionality of boar sperm capacitated in vitro. Reprod. Domest. Anim. 2002, 37, 379-380. 21.Malasa A., Faeolda R., Alagna S., Satta A., Chiarelli G., Rovasio P. P., Ivaldi R., Taras M. S., Lai E., Bartoli E.: Use of testosterone to prewent cyclophosphamide induced azoospermia. Ann. Int. Medicine 1997, 15, 292- -295.
• 22.Orzechowska-Juzwenko K.: Zarys chemioterapii nowotworów narządowych i układowych. Volumed Wrocław, Wrocław 2000.
• 23.Pennisi A. J., Grushkin C. M., Lieberman E.: Gonadal function in children with nephrosis treated with cyclophosphamide. Am. J. Dis. Child. 1975, 129, 315-318.
• 24.Saratsis Ph., Ypsilantis P., Tselkos K.: Semen quality during vincristine treatment in dogs with transmissible veneral Tumor. Theriogenology 2000, 53, 1185-1192.
• 25.Vivani S., Santoro A., Ragni G., Bonfante V., Bostetti O., Bonadonna O.: Gonadal Toxicity after combination chemotherapy for Hodgkin's disease. Comparative results of MOPP vs ABVD. Eur. J. Cancer Clin. Oncol. 1985, 21, 601-605.
• 26.Wrona Z., Krakowski L., £opuszyñski W., Kostro K., Kowalski C., Brodzki P.: Ocena płodności oraz wybranych wskażników odporności komórkowej po zastosowaniu tamoksifenu u psów. Medycyna Wet. 2003, 59, 1000-1004.