Izolacja wirusow posocznicy krwotocznej i zakaznej martwicy trzustki ryb lososiowatych w Polsce

• Autorzy: Antychowicz J , Wejman M. , Grawinski E.
• Języki publikacji: PL

Abstrakty

EN

For the first time in Poland viral examination of rainbow trout by applying cells lines (BF-2 and EPC) and ELISA tests approved in European Community Countries have shown the presence of VHS and IPN viruses existing in the country. VHS virus was isolated at 11 farms and IPN virus at 31 farms situated in northern Poland. VHS virus was isolated both in sick fish as well as in carriers and IPN virus in carriers only. The presence of VHS and IPN viruses did not appear in trout from farms in southern Poland. The experimental infection of rainbow trout demonstrated the high virulence of one of the VHS isolates. It was found that ELISA tests for VHS and IPN are very useful in detecting viruses in tissue supernatants of fish with clinical symptoms but are not as useful in detecting viruses present in cases of low titters. The results of this investigation showed that it is necessary to intensify the monitoring of the VHSV and IPNV presence in carriers and especially in fish planned to be transferred from the north to south of Poland. This should be done by using fish cell lines and immunological methods recommended in EU countries e.g. ELISA tests. The mutual co-operation of fish breeders, veterinary officers, regional fish disease laboratories and the Fish Disease Laboratory of the National Veterinary Research Institute is imperative for the effective control of fish viral diseases.

Słowa kluczowe

PL

choroby wirusowe; wirus VHS; badania wirusologiczne; izolacja wirusow; posocznica krwotoczna; etiologia; zakazna martwica trzustki; choroby zwierzat; lososiowate; wirus IPN; ryby; wykrywanie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2000
• Tom: 56
• Numer: 04
• Opis fizyczny: s.255-258,fot.,bibliogr.

Twórcy

autor

Antychowicz J

• Panstwowy Instytut Weterynaryjny, Al. Partyzantow 57, 24-100 Pulawy

autor

Wejman M.

autor

Grawinski E.

Bibliografia

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• 2. Office international des Epizooties: Diagnostic manual for aquatic animal diseases, O.I.E., 1997.
• 3. Olesen N. J., Korsholm Hr. Control measures for viral diseases in aquaculture: eradication of VHS and IHN. Bull. Eur. Ass. Fish Pathol. 1997, 17, 229-233.
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• 5. Ossiander F. J., Wedemeyer G.: Computer program for sample size required to determine disease incidence in fish populations. J. Fish. Res. Board Can., 1973, 9, 1383-1384.
• 6. Rodak L., Pospisil Z, Tománek J., Veselý T, Obr. T, Valicek T: Enzyme- -linked immunosorbent assay (ELISA) detection of infectious pancreatic necrosis virus (IPNV) in culture fluids and tissue homogenates of the rainbow trout, Salmo gairdneri Richardson. J. Fish Dis. 1988, 11, 225-235.
• 7. Rodak L., Pospisil Z., Tománek J.. Veselý T, Obr T, Valicek Tr Enzyme- -linked immunosorbent assay (ELISA) detection of spring viraemia of carp virus (SVCV) in tissue homogenates of the carp, Cyprinus carpio L. J. Fish Dis. 1993, 16, 101-111.