Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2007 | 63 | 11 Supl. |

Tytuł artykułu

Mikromacierze DNA - nowe narzedzie badawcze w fizjologii i patologii


Warianty tytułu

DNA microarray: a new tool in molecular physiology and pathology

Języki publikacji



The aim of this review is to explain fundamentals of DNA microarray technique and indicate possibilities of its use in veterinary sciences. DNA microarray technology is a powerful tool for functional and structural genomics. It allows the analysis of an entire genome expression (transcriptomic profile) on one glass or plastic slide in a short period of time. It gives the opportunity to establish genes expression patterns characteristic for different physiological and pathological conditions and allows its use as a diagnostic tool. The use of expression microarrays gives the possibility to examine the influence of new treatment strategies in particular diseases and enables the assessment of advantages and disadvantages of this type of therapy. It also allows finding new targets (key proteins, enzymes) for newly developed drugs and makes possible the adaptation of a drug therapy to individual patients. By using DNA microarray technique it is possible to make a new hypothesis and to validate current ones. In this review the origin of the DNA microarray technique, its molecular basics, different kinds of microarray slides and analysis platform as well as the methodology of labeling and hybridization were described. This review may be very useful for people who are planning to use or are have already begun using DNA microarray technique.







Opis fizyczny



  • Szkola Glowna Gospodarstwa Wiejskiego, ul.Nowoursynowska 159, 02-776 Warszawa


  • 1. Allison D. B., Cui X., Page G. P., Sabripour M.: Microarray data analysis: from disarray to consolidation and consensus. Nature Rev. Genet. 2006, 7, 55-65.
  • 2. Augenlicht L. H., Taylor J., Anderson L., Lipkin M.: Patterns of gene expression that characterize the colonic mucosa in patients at genetic risk for colonic cancer. Proc. Natl. Acad. Sci. USA 1991, 88, 3286-3289.
  • 3. Augenlicht L. H., Wahrman M. Z., Halsey H., Anderson L., Taylor J., Lipkin M.: Expression of cloned sequences in biopsies of human colonic tissue and in colonic carcinoma cells induced to differentiate in vitro. Cancer Res. 1987, 47, 6017-6021.
  • 4. Chaudhuri J. D.: Genes arrayed out for you: the amazing world of microarrays. Med. Sci. Monit. 2005, 11, RA52-62.
  • 5. Fare T. L., Coffey E. M., Dai H., He Y. D., Kessler D. A., Kilian K. A., Koch J. E., LeProust E., Marton M. J., Meyer M. R., Stoughton R. B., Tokiwa G. Y., Wang Y.: Effects of atmospheric ozone on microarray data quality. Anal. Chem. 2003, 75, 4672-4675.
  • 6. Hoheisel J. D.: Microarray technology: beyond transcript profiling and genotype analysis. Nature Rev. Genet. 2006, 7, 200-210.
  • 7. Jarzab B., Wiench M., Fujarewicz K., Simek K., Jarzab M., Oczko-Wojciechowska M., Wloch J., Czarniecka A., Chmielik E., Lange D., Pawlaczek A., Szpak S., Gubala E., Swierniak A.: Gene expression profile of papillary thyroid cancer: sources of variability and diagnostic implications. Cancer Res. 2005, 65, 1587-1597.
  • 8. Jayapal M., Melendez A. J.: DNA microarray technology for target identification and validation. Clin. Exp. Pharmacol. Physiol. 2006, 33, 496-503.
  • 9. Murawska A., Tyburski J., Budzynski T., Jarkiewicz-Tretyn J., Donderski W., Tretyn A.: Applying the microarray (chip) DNA technique for bacteriological examinations of food stuffs. Medycyna Wet. 2004, 60, 481-484.
  • 10. Pawliczak R., Kowalski M. L.: Badanie ekspresji genów metodą microarray - perspektywy wykorzystania w medycynie. Alergia Astma Immunologia 2001, 6, 77-85.
  • 11. Sadkowski T., Jank M., Oprządek J., Motyl T.: Age-dependent changes in bovine skeletal muscle transcriptomic profile. J. Physiol. Pharmacol. 2006, 57 Suppl 7, 95-110.
  • 12. Schena M., Shalon D., Davis R. W., Brown P. O.: Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 1995, 270, 467-470.
  • 13. Southern E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 1975, 98, 503-517.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.