Enzymatic redox reactions of the explosive 4,6-dinitrobenzofuroxan [DNBF]: implications for its toxic action

• Autorzy: Nemeikaite-Ceniene A. , Sarlauskas J. , Miseviciene L. , Anusevicius Z. , Maroziene A. , Cenas N.
• Języki publikacji: EN

Abstrakty

EN

With an aim to understand the toxicity mechanisms of the explosive 4,6-dinitro- benzofuroxan (DNBF), we studied its single-electron reduction by NADPH:cyto- chrome P450 reductase and ferredoxin: NADP reductase, and two- electron reduc­tion by DT-diaphorase and Enterobacter cloacae nitroreductase. The enzymatic reac­tivities of DNBF and another explosive 2,4,6-trinitrotoluene (TNT) were similar, ex­cept for the much lower reactivity of DNBF towards nitroreductase. DNBF was less cytotoxic in FLK cells than TNT. However, their action shared the same mechanisms, oxidative stress and activation by DT-diaphorase. The lower cytotoxicity of DNBF may be explained by the negative electrostatic charge of its adduct with water which may impede cellular membrane penetration, and by the formation of its less reactive adducts with intracellular reduced glutathione.

Słowa kluczowe

EN

2,4,6-trinitrotoluene; oxidative stress; cytochrome P450; cytotoxicity; DT-diaphorase; 4,6-dinitrobenzofuroxan

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 4
• Opis fizyczny: p.1081-1086,fig.,ref.

Twórcy

autor

Nemeikaite-Ceniene A.

• Institute of Immunology, Moletu Pl.29, Vilnius 2021, Lithuania

autor

Sarlauskas J.

autor

Miseviciene L.

autor

Anusevicius Z.

autor

Maroziene A.

autor

Cenas N.

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