PL
W pracy przedstawiono wyniki badania zawartości alternariolu i eteru metylowego alternariolu w surowcach i przetworach owocowych, warzywnych i zbożowych. Analizy wykonano metodą chromatografii cienkowarstwowej (TLC) i wysokociśnieniowej chromatografii cieczowej (HPLC). Stwierdzono występowanie alternariotoksyn w zapleśniałych surowcach i śladowe ich ilości w niektórych przetworach.
EN
The aim of this study was to assess Altemaria mycotoxin contamination in some raw and processed plant products. Samples were analyzed after extraction with ethyl acetate and purification on silica gel or SPE C18 columns. The final extracts on silica gel plates were determined by two-dimensional thin layer liquid chromatography and reverse phase С18 high performance liquid chromatography with UV detection. The average recoveries were 72% to 81% at 2-10 mg/kg detection limit. As much as 309 samples were analyzed: 118 raw samples (including 35 mouldy fruit and 28 low quality cereals) and 191 processed plant products. The contamination was found to be present only in evidently rotten and low quality samples. The determined levels of alternariol (AOH) ranged from 3 to 420 mg/kg, and of alternariol methyl ether (AME) from 10 to 100 µg/kg. Trace amounts of AOH were detected in 4 samples of the processed products.