NESTED PCR w diagnostyce przypadkow pneumocystozowego zapalenia pluc [PCP] u pacjentow z Aids

• Autorzy: Golab E , Sobolewska A. , Bitkowska E. , Bednarska A. , Berak H. , Dzbenski T.H. , Horban A.
• Języki publikacji: PL

Abstrakty

EN

The studies were undertaken to evaluate the usefulness of a nested PCR assay in the diagnosis of Pneumocystis carinii pneumonia (PCP) in AIDS patients. To achieve the end, 51 excretions samples from the respiratory traci were collected from HIV-infected patients with respiratory symptoms, and examined for the presence of specific DNA. A portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was amplified with outer primers pair pAZ 102E, pAZ 102H and internal primers pAZ 102X, pAZ 102Y. Positive nested PCR results were obtained with 36 out of 51 examined samples. Some 52.8% of the positive results were obtained with samples collected from patients without clinical diagnosis of PCP. It was concluded, that the nested PCR method, being too sensitive, is not suitable for the routine diagnosis of PCP in AIDS patients.

Słowa kluczowe

PL

zapalenie pluc; metody diagnostyczne; czynniki chorobotworcze; choroby czlowieka; parazytologia lekarska; lancuchowa reakcja polimerazy; pneumocystoza; diagnostyka; AIDS; ludzie chorzy; Pneumocystis carinii; metoda nested-PCR

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2001
• Tom: 47
• Numer: 3
• Opis fizyczny: s.521-525,tab.,bibliogr.

Twórcy

autor

Golab E

• Zaklad Parazytologii Lekarskiej, ul.Choocimska 24, 00-791 Warszawa

autor

Sobolewska A.

autor

Bitkowska E.

autor

Bednarska A.

autor

Berak H.

autor

Dzbenski T.H.

autor

Horban A.

Bibliografia

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• LIPSCHIK G. Y, GILL V. J., LUNDGREN J. O., ANDRAWIS V. A., NELSON N. A., NIELSEN J. O., OGNIBENE F. P., KOVACS J. A. 1992. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 340: 203-206.
• RABODONIRINA M., RAFFENOT D., COTTE L., BOIBIEUX A., MAYENCON, M., BAYLE O., PERSAT F., RABATEL F., TREPO C., PEYRAM0ND D., PIENS M-A. 1997. Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimens from Human Immunodeficiency Virus- infected patients: use of a simple DNA extraction procedure and nested PCR. J. Clin. Microbiol. 35: 2748-2751.
• TAMBURRINI E., MENCARINI P., VISCONTI E., ZOLFO M., DE LUCA A., SIRACUSANO A., ORTONA E., WAKEFIELD A. E. 1996. Detection of Pneumocystis carinii DNA in blood by PCR is not of value for diagnosis of P. carinii pneumonia. J. Clin. Microbiol. 34: 1586-1588.
• WAKEFIELD A. E., PIXLEY F. J., BANERJI S., SINCLAIR K., MILLER R. F., MOXON E. R., HOPKIN J. M. 1990. Detection of Pneumocystis carinii with DNA amplification. Lancet 336: 451-453.