Expression of genes 51, 27, 28 coding for proteins of the central part of bacteriophage T4 baseplate in the bacteriophage T7 promoter-RNA polymerase expression system

• Autorzy: Nieradko J. , Podgorska B.
• Języki publikacji: EN

Abstrakty

A fragment of T4 DNA (XbaI-HindIII) comprising the genes 51,27,28, which encodes the central plug proteins was cloned into plasmid pT7-5 and p7-6 (T7 RNA polymerase expressing system). The examined genes were only overexpressed when the orientation of cloned DNA to promoter <1>10 was as follows: promoter 10 and genes 51, 27, 28. This was achieved when the fragment (Xbal-Hindlll) was cloned into plasmid pT7-5. Gene 27 and 28 were overexpressed when the intact fragment (Xbal-Hindlll) was used. The high rate of the synthesis of proteins 27 and/or 28 had a strong inhibitory effect on the level of synthesis of the product of gene 51. For the overexpression of gene 51 in this system a deletion derivate which was devoid of gene 28 and a larger fragment of gene 27 was prepared.

Słowa kluczowe

EN

bacteriophage; expression system; gene; gene expression; protein; RNA polymerase

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1993
• Tom: 40
• Numer: 2
• Opis fizyczny: p.273-278,fig.

Twórcy

autor

Nieradko J.

• Gdansk University, Kladki 24, 80-822 Gdansk, Poland

autor

Podgorska B.

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