Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex

• Autorzy: Humphreys M W , Thomas H M , King I P , Morgan W G , Meredith M R , Harper J A , Humphreys M O , Bettany A J E , Dalton S J , James A R , Ougham H J , Thomas H
• Języki publikacji: EN

Abstrakty

EN

Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.

Słowa kluczowe

EN

RNA; in situ; tissue culture; gene transfer; chromosome segment; anther culture; androgenesis; plant breeding; introgression mapping; fluorescence; hybridization; Lolium-Festuca complex; drought resistance; gene isolation; tetraploid hybrid; DNA; meiosis; grass; chromosome behaviour

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 3
• Opis fizyczny: p.273-284

Twórcy

autor

Humphreys M W

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

Thomas H M

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

King I P

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

Morgan W G

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

Meredith M R

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

Harper J A

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

Humphreys M O

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

Bettany A J E

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

Dalton S J

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

James A R

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

Ougham H J

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

autor

Thomas H

• Institute of Grassland and Environmental Research, Plas Gogerddan, Nr.Aberystwyth, Ceredigion, SY23 3EB, U.K.

Bibliografia

• Dalton S J., Bettany A.J.E., Timms E., Morris P. (1995).The effect of selection pressure on transformation frequency and copy number in transgenic plants of tall fescue (Festuca arundinacea Schreb.). Plant Sci. 108: 63-70.
• Gerlach W.L., Bedbrook J.R. (1979). Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucl. Acids Res. 7: 1869-1885.
• Gerlach W.L., Dyer T.A. (1980). Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes. Nucl. Acids Res. 8: 4851-4855.
• Ghesquiere M., Durand F., Le Quilliec P., Gaullier F. (1991). Use of electrophoretic markers in chromosome manipulations of Festuca x Lolium hybrids. In: Proc. EUCARPIA Fodder Crops Sect. Meet. Algero, Italy.
• Hayward M.D., McAdam N.J. (1988). The effect of isozyme selection on yield and flowering time on Lolium perenne. Plant Breeding 101: 24-29.
• Hayward M.D., McAdam N.J., Jones J.G., Evans C., Evans G.M., Forster J.W., Ustin A., Hossain K.G., Quader B., Stammers M., Will J.K. (1994). Genetic markers and the selection of quantitative traits in forage grasses. Euphytica 77: 269-275.
• Hubank M., Schatz D.G. (1994). Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucl. Acids Res. 22: 5640-5648.
• Humphreys M.O. (1992). Association of agronomic traits with isozyme loci in perennial ryegrass (Lolium perenne L.). Euphytica 59: 141-150.
• Humphreys M.O. (1993). Genetic resources for improved climatic adaptation within the ryegrass/fcscue complex. In: Proc. COST 814 Workshop, Aberystwyth: 281-287.
• Humphreys M.O., Evans C., Farrell M.S., Jones J.G., McAdam N.J., Hayward M.D. (1995). Targets for marker assisted selection in grass breeding. In: Grassland into the 21st. Century: Challenges and Opportunities (G.E. Pollolt, ed.). Occasional Symposium No. 29, British Grassld. Soc.: 236-237.
• Humphreys M.O., Honne B.I. (1995). Potential for improved winterhardiness in ryegrass through hybridisation with fescues. In: Adaptation in Plant Breeding. Proc. XIV EUCARPIA Congress, Jyvaskyla, Finland, July 31-August 4, 1995. University of Jyvaskyla: 79.
• Humphreys M.O., Thomas H., Tyler B.F. (1989). The potential of Lolium multiflorum x Festuca gigantea. In: Proc. XVI Int. Grassld. Congr. (R. Dcsrochcs, ed.). Nice, France, 1: 253-254.
• Humphreys M.W. (1989). The controlled introgression of Festuca arundinacea genes into Lolium multiflorum. Euphytica 42: 105-116.
• Humphreys M.W. (1995). Gene introgression following intergencric hybridization within the Lolium/Festuca complex. PhD Thesis, University of Wales, Aberystwyth: 200-228.
• Humphreys M.W., Dalton S.J. (1992). Stability at the phosphogluoisomerase (PGI/2) locus in Lolium multiflorum (2n=4x=28) x Festuca arundinacea (2n= 6x=42) plants regenerated from cell suspension. Genome 35: 461-467.
• Humphreys M.W., Ghesquiere M. (1994). Assessing success in gene transfer between Lolium multiflorum and Festuca arundinacea. Euphytica 77: 283-289.
• Humphreys M.W., Pasakinskiene I. (1996). Chromosome painting to locate genes for drought resistance transferred from Festuca arundinacea into Lolium multiflorum. Heredity 77: 530-534.
• Humphreys M.W., Thomas H. (1993). Improved drought resistance in introgression lines derived from Lolium multiflorum x Festuca arundinacea hybrids. Plant Breeding 111: 155-161.
• Humphreys M.W., Thomas H., Humphreys M.O. (1997). Improvement of drought resistance in Italian ryegrass by introgression from tall fescue. Proc. Physical Mapping of Plant Chromosomes. Univ. of Wales, Aberystwyth, UK. 8-10 January 1997: 46.
• Humphreys M.W., Thomas H.M., Morgan W.G., Meredith M.R., Harper J. A., Thomas H., Zwierzykowski Z., Ghesquiere M. (1995). Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in silu hybridization. Heredity 75: 171-174.
• Humphreys M.W., Thorogood D. (1993). Disturbed Mendelian segregations at isozyme marker loci in early backcrosses of Lolium multiflorum x Festuca pratensis hybrids to L. multiflorum. Euphytica 66: 11-18.
• Humphreys M.W., Zare A.G., Pasakinskiene I., Kerlan M-C. (1996). The potential of anther culture derived plants from a L. multiflorum x F. arundinacea (2n=5x= 35) hybrid as a novel forage grass crop. Proc. 20th. Meet. EUCARPIA Fodder Crops and Amenity Grasses Section, Radzików, Poland, 1996: 155-156.
• Jiang J., Gill B.S. (1994). New 18S.26S ribosomal RNA gene loci: chromosomal landmarks for the evolution of polyploid wheats. Chromosoma 103: 179-185.
• Liang P., Pardee A.B. (1992). Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257: 967-971.
• Lisitsyn N.A. (1995). Representational difference analysis: finding the differences between genomes. Trends Genet. 11: 303-307.
• Morgan W.G., Thomas H., Lewis E.J. (1988). Cytogenetic studies of hybrids between Festuca gigantea Vill. and Lolium multiforum Lam. Plant Breeding 101: 335-343.
• Pasakinskiene I., Anamthawat-Jonsson K., Humphreys M.W., Jones R.N. (1997). Novel diploids following chromosome elimination and somatic recombination in Lolium multiflorum x Festuca arundinacea hybrids. Heredity (in press).
• Thomas H., Humphreys M.O. (1991). Progress and potential of interspecific hybrids of Lolium and Festuca. J. Agric. Sci. Cambridge 117: 1-8.
• Thomas H., Humphreys M.W., Ghesquiere M., Humphreys M.O., Mousset C. (1995). Improved drought resistance in Lolium by introgression from Festuca. Proc. of the IntcrDrought’95 Conference, Montpellier, France, 1995, IIA.
• Thomas H.M. (1981). The giemsa c-band karyotypes of six Lolium species. Heredity 46(2): 263-267.
• Thomas H.M., Harper J.A., Meredith M.R., Morgan W.G., Thomas I.D.,Timms E., King I.P. (1996). Comparison of ribosomal DNA sites in Lolium species by fluorescent in situ hybridization. Chromosome Res. 4: 486-490.
• Thomas H.M., Morgan W.G., Meredith M.R., Humphreys M.W., Thomas H., Leggett J.M. (1994). Identification of parental and recombined chromosomes in hybrid derivatives of Lolium multiflorum x Festuca pratensis by genomic in situ hybridization. Theor. Appl. Genet. 88: 909-913.