Cytogenetic effects of Fusarium mycotoxins on root tip cells of rye [Secale cereale L.], wheat [Triticum aestivum L.] and field bean [Vicia faba L.var.minor]

• Autorzy: Packa D
• Języki publikacji: EN

Abstrakty

EN

The effects of fusarial toxins: DAS, T-2, DON, 3 Ac-DON, MON and ZEA, on actively dividing root tip cells of rye, wheat and field bean were investigated. Three concentrations: 1, 5 and 10 µg mL⁻¹ were applied for 24 hours. Nuclei and chromosomes were stained using the Feulgens method. It has been found that trichothecene mycotoxins (DAS, T-2, DON, 3 Ac-DON) had a profound effect on mitosis, as they decreased the mitotic index, produced excessive condensation of pro- and metaphase chromosomes, C-metaphascs and C-anaphases, and caused an accumulation of metaphases. The main effect of trichothecene mycotoxins, probably caused via their influence on protein synthesis, was abnormal functioning of the mitotic spindle.

Słowa kluczowe

EN

mycotoxin; rye; rot tip; fumonisin B1; fumonisin B3; mitosis; Triticum aestivum; fungi; tip cell; Fusarium; chromosome; wheat; Secale cereale; bean; fumonisin B2; Vicia faba var.minor; plant tissue; metaphase; moniliformin

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 3
• Opis fizyczny: p.259-272,fig.

Twórcy

autor

Packa D

• Department of Plant Breeding and Seed Production, University of Agriculture and Technology, Pl.Lodzki 3, 10-724 Olsztyn, Poland

Bibliografia

• Asch van M.A.J., Rijkenberg F.H.J., Coutinho T.A. (1992). Phytotoxicity of fumonisin B₁, moniliformin and T-2 loxin to corn callus cultures. Phytopathology 82(11): 1330-1332.
• Betina V. (ed.) (1984). Mycotoxins-production, isolation, separation and purification. Elsevier Sci. Publ. B.V., Amsterdam.
• Bilgrami K.S. (1991). Histopathological and cytological abnormalities induced by fusarial toxins in mic (Mus musculus). Mycotoxin Research 7, A, part 1: 12-16.
• Casale W.L., Hart L.P. (1986). Inhibition of protein synthesis in maize by trichothecene mycotoxins (Abstract). Phytopathology 76(10): 1144.
• Casale W.L., Hart L.P. (1988). Inhibition of ³H-leucine incorporation by trichothecene mycotoxins in maize and wheat tissue. Phytopathology 78(12): 1673-1677.
• Chełkowski J. (ed.) (1989). Fusarium: mycotoxins, taxonomy and pathogenicity. Elsevier Sci. Publ. B.V., Amsterdam.
• Chełkowski J. (1994). Significance of Fusarium metabolites in interaction between a cereal plant and a pathogen. The review. Genet. Pol. 35B: 137-142.
• Gerlach D. (1972). Zarys mikrotechniki botanicznej. Państwowe Wydawnictwo Rolnicze i Leśne, Warszawa.
• Hsia C.C., Gao Y., Wu J.L., Tzian B.L. (1986). Induction of chromosome aberrations by Fusarium T-2 toxin in cultured human peripheral blood lymphocytes and Chinese hamster fibroblasts. J. Cell. Physiol. Suppl. 4: 65-72.
• Joffe A.Z. (1986). Fusarium species: Their biology and toxicology. John Wiley and Sons, Inc. New York, Chichester.
• Kihlman B.A. (1966). Action of chemicals on the dividing cells. Prentice - Hall Inc., New Jersey.
• Kihlman B.A. (1975). Root tips of Vicia faba for the study of the induction of chromosomal aberrations. Mutat. Res. 31: 401-412.
• Kirsch-Volders M., Parry E.M. (1996). Genetic toxicology of mitotic spindle inhibitors used as anticancer drugs. Mutat. Res. 355: 103-128.
• Kurata H., Ueno Y. (eds.) (1984). Toxigenic fungi, their toxins and health hazard. Kodansha Ltd. Tokyo and Elsevier Sci. Publ. B.V., Amsterdam.
• Lacey J. Ed. (1985). Trichothecenes and other mycotoxins. John Wiley and Sons, Inc., Chichester.
• Lafarge-Frayssinet С., Decloitre K, Mousset S., Martin M., Frayssinet C. (1981). Induction of DNA single-strand breaks by T-2 toxin, a trichothecene metabolite of Fusarium. Effect on lymphoid organs and liver. Mutat. Res. 88: 115-123.
• Lamprecht S.C., Marasas W.F.O., Alberts J.F., Cawood M.E., Gelderblom W.C.A., Shephard G.S., Thiel D.G., Calitz F.J. (1994). Phytotoxicity of fumonisins and TA-toxin to corn and tomato. Phytopathology 84(4): 383-391.
• Linnainmaa K., Sorsa M., Ilus T. (1979). Epoxytrichothecene mycotoxins as c-mitotic agents in Allium. Hereditas 90: 151-156.
• Мa T.H. (1982). Vicia cytogenetic tests for environmental mutagens. Mutat. Res. 99: 257-271.
• Miller J.D. (1989). Effects of Fusarium graminearum metabolites on wheat cells. In.: Phytotoxins and Plant Pathogenesis (A. Graniti, R.D. Durbin, A. Ballio, eds.) Springer-Verlag, Berlin, Heidelberg.
• Miller J.D., Trenholm H.L. (eds.) (1994). Mycotoxins in grain. Compounds other than aflatoxin. Eagan. Press. St. Paul, Minnesota, U.S.A.
• Moule Y. (1984). Biochemical effects of mycotoxins. In: Mycotoxins-Production, Isolation, Separation and Purification (V. Betina, ed.). Elsevier Sci. Publ. B.V., Amsterdam.
• Packa D. (1994). Wpływ toksyn fuzaryjnych na przebieg mitozy u zbóż i bobiku. Hod. Rośl. Nasien. 1: 3-8.
• Perkowski J. (1993). Tworzenie mikotoksyn w zbożach przez grzyby rodzaju Fusarium. PNR 2: 67-82.
• Pfohl-Leszkowicz A., Chekir-Ghedira L., Bacha H. (1995). Gcnotoxicity of zearalenonc an estrogenie mycotoxin: DNA adduct formation in female mouse tissues. Carcinogenesis 16(10): 2315-2320.
• Snijders C.H.A. (1990). Fusarium head blight and mycotoxin contamination of wheat, a review. In: Aspects of Resistance to Fusarium Head Blight Caused by Fusarium culmorum in Wheat. Landbouwuniversiteit Wageningen: 5-15.
• Styer C.H., Cutler H.G. (1984). Effects of moniliformin on mitosis in maize (Zea mays L.). Plant Cell Physiol. 25(6): 1077-1082.
• Thust R., Kneist S., Hühne V. (1983). Gcnotoxicity of Fusarium mycotoxins (nivalenol, fusarenon-X, T-2 toxin and zearalenone) in Chinese hamster V79-E cells in vitro. Arch. Geschwulstforsch 53(1): 9-15.
• Ueno Y., Ishii K. (1985). Chemical and biological properties of trichothecenes from Fusarium sporotrichioides. In: Trichothecenes and Other Mycotoxins (J. Lacey, ed.). John Wiley and Sons Inc., Chichester.
• Ueno Y., Sato N., Ishii K., Sakai K., Tsunoda H., Enomoto M. (1973). Biological and chemical detection of trichothecene mycotoxins of Fusarium species. Appl. Microbiol. 25(4): 699-704.
• Umeda M. (1977). Cytotoxicity of mycotoxins. In: Mycotoxins in Human and Animal Health (J.V. Rodricks, C.W. Hesseltine, M.A. Mehlman, eds.). Pathotox Publishers Inc.
• Vesonder R.F., Goliński P. (1989). Metabolites of Fusarium. In: Fusarium Mycotoxins, Taxonomy and Pathogenicity (J. Chełkowski, ed.). Elsevier Sci. Publ. B.V., Amsterdam.
• Vesonder R.F., Hesseltine C.W. (1981). Metabolites of Fusarium. In: Fusarium Mycotoxins, Taxonomy and Pathogenicity (P.E. Nelson, T.A. Toussoun, R J. Cook, eds.). Pennsylvania State University Press.
• Wakuliński W. (1989). Phytotoxicity of Fusarium metabolites in relation to pathogenicity. In: Fusarium Mycotoxins, Taxonomy and Pathogenicity (J. Chełkowski, ed.). Elsevier Sci. Publ. B.V., Amsterdam.
• Wakuliński W. (1990). Phytotoxicity of the secondary metabolites of fungi causing wheat head fusariosis (head blight). Acta Physiol. Plant. 11(4): 301-306.
• Wakuliński W., Chełkowski J. (1992a). Fusarium species transmitted with seed of wheat, rye, barley, oats and triticale. Hod. Rośl. Aklim. 37(4): 131-136.
• Wakuliński W., Chełkowski J. (1992b). Fusarium species causing scab of wheat, rye, and triticale in Poland. Hod. Rośl. Aklim. 37(4): 137-142.
• Wang Y.Z., Miller J.D. (1988). Effects of Fusarium graminearum metabolites on wheat tissue in relation to Fusarium head blight resistance. J. Phytopathology 122: 118-125.
• Wiśniewska H., Chełkowski J. (1994). Influence of dcoxynivalcnol on mitosis of root tip cells of wheat seedlings. Acta Physiol. Plant. 3: 159-162.