Analiza molekularna genu hemaglutyniny wirusa nosowki izolowanego z klinicznych przypadkow choroby od lisow i psow

• Autorzy: Adaszek L , Winiarczyk S. , Jankowski L. , Maj J. , Zietek J.

Warianty tytułu

EN

Molecular analysis of CDV hemagglutinin gene isolated from clinical cases of distemper in foxes and dogs

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to to detect and characterize the canine distemper virus isolated from 4 foxes and 6 dogs, naturally infected in Poland, as well as from two vaccines. A 200-bp fragment of the CDV H gene was amplified and sequenced. Sequencing of the PCR products from the isolates led to the identification of 4 groups. The most numerous group No. 1 was represented by virus strains isolated from 4 foxes and 4 dogs. In group No. 2 there were two sequences of CDV isolated from two dogs. These sequences varied from group 1 by transitions in positions 133 and 135. The isolates from groups 1 and 2 showed significant differences of nucleotide as well as aminoacid sequences in comparison with the isolates obtained from vaccines and with various CDV sequences obtained from GenBank. The results may indicate the appearance of new CDV variants in eastern Poland.

Słowa kluczowe

PL

psy; lisy; choroby zwierzat; nosowka; zwierzeta chore; wirus nosowki; gen hemaglutyniny; polimorfizm; analiza molekularna; lancuchowa reakcja polimerazy; metoda RT-PCR; sekwencjonowanie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2009
• Tom: 65
• Numer: 07
• Opis fizyczny: s.475-479,rys.,tab.,bibliogr.

Twórcy

autor

Adaszek L

• Uniwersytet Przyrodniczy w Lublinie, ul.Gleboka 30, 20-612 Lublin

autor

Winiarczyk S.

autor

Jankowski L.

autor

Maj J.

autor

Zietek J.

Bibliografia

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