The contribution of uncoupling protein and ATP synthase to state 3 respiration in Acanthamoeba castellanii mitochondria

• Autorzy: Jarmuszkiewicz W. , Czarna M. , Sluse-Goffart C. , Sluse F.E.
• Języki publikacji: EN

Abstrakty

EN

Mitochondria of the amoeba Acanthamoeba castellanii possess a free fatty acid-ac­tivated uncoupling protein (AcUCP) that mediates proton re-uptake driven by the mi­tochondrial proton electrochemical gradient. We show that AcUCP activity diverts energy from ATP synthesis during state 3 mitochondrial respiration in a fatty acid-dependent way. The efficiency of AcUCP in mitochondrial uncoupling increases when the state 3 respiratory rate decreases as the AcUCP contribution is constant at a given linoleic acid concentration while the ATP synthase contribution decreases with respiratory rate. Respiration sustained by this energy-dissipating process remains constant at a given linoleic acid concentration until more than 60% inhibition of state 3 respiration by n-butyl malonate is achieved. The present study supports the validity of the ADP/O method to determine the actual contributions of AcUCP (activated with various linoleic acid concentrations) and ATP synthase in state 3 respiration of A. castellanii mitochondria fully depleted of free fatty acid-activated and describes how the two contributions vary when the rate of succinate dehydrogenase is decreased by succinate uptake limitation.

Słowa kluczowe

EN

linoleic acid; bovine serum albumin; mitochondrion; amoeba; uncoupling protein; Acanthamoeba castellanii; benzohydroxamate; respiratory control ratio; free fatty acid

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 2
• Opis fizyczny: p.533-538,fig.,ref.

Twórcy

autor

Jarmuszkiewicz W.

• Adam Mickiewicz University, A.Fredry 10, 61-701 Poznan, Poland

autor

Czarna M.

autor

Sluse-Goffart C.

autor

Sluse F.E.

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