Detection of two nucleotide substitutions and putative promoters in the 5' flanking region of the ovine IGF-I gene

• Autorzy: Yilmaz A , Davis M.E. , Hines H.Ch. , Chung H.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to search for polymorphisms and gene regulatory sequences in the 5' flanking region of the sheep insulin-like growth factor I (IGF-I) gene. PCR-SSCP analysis of the 5' flanking region revealed three banding patterns. Family study indicated that these patterns in mixed breed sheep corresponded with three genotypes (with their frequencies in parentheses) A A (0.70), AB (0.25), and BB (0.05), which arose from a one-locus, two allele (A, B) polymorphism. Genotypie frequencies in 22 purebred Polypay sheep were AA (0.77) and AB (0.23). Calculated frequency of the A allele in Polypays was 0.89. No deviation from Hardy-Weinberg equilibrium was detected in this study. Fragments amplified using DNA from homozygous individuals were sequenced and aligned next to each other. A T to C transition and a G to C transversion were found at positions 179 and 181, respectively, of the amplified PCR product, resulting in recognition sites for Bsp143II and HaeI. Analysis of a fragment of 2,162 base pairs upstream of Exon 1, assembled from sheep ESTs and sequence of our amplified PCR products, revealed a promoter sequence approximately 100 bp downstream of the polymorphic sites. The assembled DNA fragment shared 70% sequence homology between sheep and human. These results suggest that sequence of the 5' flanking region of IGF-I gene and location of the IGF-I promoters are similar in human and sheep.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2005
• Tom: 46
• Numer: 3
• Opis fizyczny: p.307-309,fig.,ref.

Twórcy

autor

Yilmaz A

• The Ohio State University, 221 Plumb Hall, 2027 Coffey Road, Columbus, OH 43210

autor

Davis M.E.

autor

Hines H.Ch.

autor

Chung H.

Bibliografia

• Adam CL, Gadd TS, Findlay PA, Wathes DC, 2000. IGF-I stimulation of luteinizing hormone secretion, IGF-binding proteins (IGFBPs) and expression of mRNAs for IGFs, IGF receptors and IGFBPs in the ovine pituitary gland. J Endocrinol 166: 247-254.
• Ge W, Davis ME, Hines HC, 1997. Two SSCP alleles detected in the 5'-flanking region of bovine IGF1 gene. Anim Genet 28: 155-156.
• Ge W, Davis ME, Hines HC, Irvin KM, Simmen RC, 2001. Association of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. J Anim Sci 79: 1757-1762.
• Grochowska R, Sorensen P, Zwierzchowski L, Snochowski M, Lovendahl P. 2001. Genetic variation in stimulated GH release and in IGF-I of young dairy cattle and their associations with the leucine/valine polymorphism in the GH gene. J Anim Sci 79: 470-476.
• Reese MG, 2000. Computational prediction of gene structure and regulation in the genome of Drosophila melanogaster. Ph.D. Dissertation, University of California at Berkeley.
• Reese MG, 2001. Application of a time-delay neural network to promoter annotation in the Drosophila melanogaster genome. Comput Chem 26: 51-56.
• Shen W, Wisniowski P, Ahmed L, Boyle DW, Denne SC, Liechty EA, 2003. Protein anabolic effects of insulin and IGF-I in the ovine fetus. Am J Physiol Endocrinol Metab 284: E748-756.
• Wong EA, Ohlsen SM, Godfredson JA, Dean DM, Wheaton JE, 1989. Cloning of ovine insulin-like growth factor-I cDNAs: heterogeneity in the mRNA population. DNA 8: 649-655.