Sklad podklas IgG i ich aktywnosc biologiczna w bioglobulinie

• Autorzy: Bucholc B. , Banach W.
• Języki publikacji: PL

Abstrakty

PL

W prezentowanej pracy określono skład podklas IgG w preparatach ludzkich immunoglobulin do stosowania dożylnego w tym w preparacie wyprodukowanym przez Biomed-Warszawa. Stwierdzono różnice w składzie podklas IgG1 i IgG2 między Bioglobuliną a pozostałymi preparatami. Różnice w składzie podklas nie wpływały na aktywność badanych przeciwciał bakteryjnych i wirusowych.

EN

Preparations of human immunoglobulin for intravenous use (IVIG) should contain proper percentage of IgG subclasses, corresponding to physiological proportions with preserved activity of antibodies. The amounts of IgG subclasses were determined using the radial immunodiffusion technique against to the WHO reference serum 67/97. It appears that variations are observed among the different lots and manufacturers of the various formulations of IVIG available in Poland. Although some of these differences are not statistically significant, significant differences in subclass concentrations were found between formulation of IVIG, with Bioglobulina being lower in IgG1 and higher in IgG2 and Endobulin having a lower per cent of IgG3. The study was aimed at establishment of activity values against diphtheria and tetanus toxoids and Streptococcus group B (GBS ). IgG antibodies to tetanus and diptheria toxoid antigens were predominantly of the IgG1 and IgG2 isotype. IgG4 subclass, was present in smaller proportion and IgG3 fractions was absent. Antibodies to GBS were only IgG2 and IgG1 isotypes. Basing on obtained results it can be stated that in spite of the imbalance between the amounts of IgGI1 and IgG2 in Bioglobulina, the activity which was determined in subclasses is comparable with that of other IVIGs.

Słowa kluczowe

PL

immunoglobulina G; preparat Bioglobulina; przeciwciala; immunoglobuliny; aktywnosc biologiczna

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 1998
• Tom: 50
• Numer: 1-2
• Opis fizyczny: s.105-113,tab.,bibliogr.

Twórcy

autor

Bucholc B.

• Panstwowy Zaklad Higieny, 00-791 Warszawa, ul.Chocimska 24

autor

Banach W.

Bibliografia

• 1. Aalberse RC, Gaag R, van der Leeuwen J. Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4 restricted response. J Immunol 1983; 130: 722-26.
• 2. Aucouturier P, Barra A, Intrator L i inni. Long lasting IgG subclass and antibacterial polysaccharide antibody deficiency after allogenic bone marrow transplantation. Blood 1987; 70: 779-85.
• 3. Bernatowska-Matuszktewicz E, Pac M, Skopczyńska H i inni. Clinical efficacy of intravenous immunoglobulin in patients with severe inflammatory chest disease and IgG3 subclass deficiency. Clin Exp Immunol 1991; 85: 193-97.
• 4. Brekke OH, Michaelsen TE, Dandlie I. The structural requirements for complement activa¬tion by IgG does it hinge on the hinge? Immunol Today 1995; 16: 85-90.
• 5. Burton DR, Gregory L, Jefferis R. aspects of the molecular structure of IgG subclasses. Monogr Allegry 1986; 19: 7-35.
• 6. Bucholc B, Banach W. Synergistyczne działanie penicyliny i immunoglobulin do stosowania dożylnego. Med Dośw Mikrobiol 1996; 48: 201-10.
• 7. Falloon J, Eddy J, Wiener LP i inni. Human immunodeficiency virus infection in children. J Pediatr 1989; 114: 1-23.
• 8. Ferrante A, Beard LJ, Feldman RG. IgG subclass distribution of antibodies to bacterial and viral antigens. Pediatr Infect Dis J 1990; 9: 16-24.
• 9. French MAH, Harrison G. The IgG subclasses of tetanus toxoid antibody following immu¬nization: studies in healthy adults and patients with chronic or recurrent respiratory tract infections. J Clin Lab Immunol 1985; 18: 21-5.
• 10. Granoff DM, Shackelford PG, Suarez BK i inni. Haemophilus influenzae type B disease in children vaccinated with type polysaccharide vaccine. N Engl J Med 1988; 315: 1584-90.
• 11. Gregorek H, Pietrucha B, Janowicz W i inni. IgG subclass deficiencies in children with recurrent or respiratory tract infections. Pol J Immunol 1993; 18: 319-28.
• 12. Hamilton RG. Human IgG subclass measurements in the clinical laboratory. Clin Chem 1987; 33: 1707-25.
• 13. Hammarstrom L, Lefranc G, Lefranc MP i inni. Aberrant pattern of anticarbohydrate antibodies in immunoglobulin class or subclass-deficient donors. Monogr Allergy 1986; 20; 50-56.
• 14. Heiner DC, Lee SI, Short JA. IgG4 subclass deficiency syndromes. Monogr Allergy 1986; 20: 149-56.
• 15. Kurniawan A, Yazdanbakhsh M, Van Ree R i inni. Differential expression of IgE and IgG4 specific antibody responses in asymptomatic and chronic human filariasis. J Immunol 1993; 15: 1-10.
• 16. Litwińska B, Bucholc B, Biesiadecka A, Kańtoch M. Aktywność przeciwwirusowa w podklasach IgG preparatów immunoglobulin do stosowania dożylnego. Med Dośw Mikrobiol 1993; 45: 523-28.
• 17. Oxelius VA. Immunoglobulin G (IgG) subclasses and human disease. Am J Med 1984; 76: 7-18.
• 18. Oxelius VA, Eibl MM. Different Gm allotype amounts in human intravenous immunoglobulin (IVIG) preparations; survival of foreign Gm allotypes in immunodeficients patients. Clin Exp Immunol 1996; 106: 203-07.
• 19. Sarvas H, Rautonen N, Sipinen S i inni. IgG subclasses of pneumococcal antibodies - effects of allotype G2m(n). Scand J Immunol 1989; 29: 229-37.
• 20. Scott MG, Shackelford PG, Briles DE i inni. Human IgG subclasses and their relation to carbohydrate antigen immunocompetence. Diagn Clin Immunol 1988; 5: 241-48.
• 21. Skvaril F. IgG subclasses in viral infections. Monogr Allergy 1986; 19: 134-43.
• 22. Skvaril F. Clinical relevance of IgG subclasses. W: Clinical use of intravenous immunoglo¬bulins. Red. Morell. A., Nydegger. U.E., London, Academic, 1986, 37-45.
• 23. Zakrzewska A, Rymkiewicz D. Ocena aktywności antytoksycznej i antybakteryjnej preparatów ludzkiej normalnej immunoglobuliny I. Poziom antytoksyn tężcowych w preparatach IMIG i IVIG. Przegl Epid 1995; 49: 381-84.
• 24. Yount WJ, Dorntner MM, Kunkel HG i inni. Studies on human antibodies IV. Selective variations in subgroup composition and genetic markers. J Exp Med 1968; 127: 633-46.