EN
The aim of this paper was the detection of the specific antibodies anti-Borrelia and anti-Ehrlichia in the sera of 28 sick pigs and 29 sick cows from the area of the Lublin voivodeship. ELISA tests, and Western blot were conducted. In ELISA tests, 17.86% sera of swine were positive for spirochetes and 7.14% for Ehrlichia. In a bovine group 44.83% of sera specimens were positive for Borrelia, and 20.69% for Ehrlicha. All sera that were positive in ELISA tests for spirochetes were examined additionally in Western blot method for the presence of antibodies against Borrelia afzelii (13 bovine sera, and 5 swine sera). In 8 out of 13 bovine sera (61.54%) and in one out of 5 (20%) swine sera that were positive in the ELISA test, specific antibodies against Borrelia were detected. In all cases IgG immunoglobins reacted strongly with the antigens: 31, 39, 41, 66 kDa of B. afzelii. The results revealed a low specificity of the ELISA test for the detection of borreliosis and confirmed an endemic occurrence of Borrelia and Ehrlicha in the area of the Lublin voivodeship