Analysis of changes in selected parameters of calcium and magnesium metabolism in response to diet composition and B-group vitamin supplementation in rat

• Autorzy: Sadowska J.

Warianty tytułu

PL

Analiza zmian wybranych wskaznikow gospodarki wapniem i magnezem zachodzacych pod wplywem skladu diety oraz suplementacji witaminami z grupy B u szczura

• Języki publikacji: EN

Abstrakty

EN

Background. The aim of the study was to investigate the effects of a diet modification and supplementation with B-group vitamins, on selected characteristics of calcium and magnesium management in rats. Material and methods. The experiment was carried out on 60 rats aged 5 months. Animals were fed two different diets. Groups I and II received clean tap water to drink, while group III had water solution of group-B vitamins. Results. Analysis of blood plasma calcium and magnesium concentrations in the studied animals did not reveal a significant effect of the analysed factors on blood plasma calcium concentration in examined rats. An increase of the plasma level of magnesium was observed with a change in the diet composition. The supplementation reduced magnesium level to those observed in animals fed a basic feed. Diet modification and supplementation exerted the influence on whole blood calcium and magnesium levels. A change in the composition of the diet and its supplementation results also in an increase in bone calcium content in males, and in an increase in bone magnesium content in females. Conclusions. Lack of changes in blood plasma calcium levels in the studied animals implies the preservation of the homeostatic mechanisms that regulate its concentration, whereas the observed significant changes in the concentration of magnesium, point to a significant effect of this factor on its metabolism. Changes in hematocrit indicator, whole blood concentrations of calcium and magnesium and the absence of changes in concentrations of these elements in blood plasma of supplemented animals may indicate that the elements move to erythrocytes, which may imply a distortion of cellular membrane and an increase in its permeability. Composition of the diet and its supplementation modified also bone calcium and magnesium concentrations in the studied rats.

PL

Wstęp. Celem badań była ocena wpływu składu diety oraz suplementacji witaminami z grupy B na wybrane wskaźniki gospodarki wapniem i magnezem u szczura. Materiał i metody. Badania przeprowadzono na 60 szczurach w wieku 5 miesięcy. Zwierzęta żywiono dwiema paszami o różnym składzie. Do picia grupy I i II otrzymywały czystą wodę, grupie III podawano roztwór witamin z grupy B. Wyniki. Nie stwierdzono istotnego wpływu badanych czynników na stężenie wapnia w osoczu badanych zwierząt. Zmiana składu diety wpłynęła na wzrost stężenia magnezu w osoczu. Zastosowana suplementacja zmniejszała stężenie do poziomu obserwowanego u zwierząt żywionych paszą podstawową. Modyfikacja diety i suplementacja wywarły wpływ na stężenie wapnia i magnezu w pełnej krwi badanych zwierząt. Zmiana składu diety i zastosowana suplementacja oddziaływały także na wzrost zawartości wapnia w kościach samców oraz magnezu w kościach samic. Wnioski. Brak zmian w stężeniu wapnia w osoczu badanych zwierząt wskazuje na zachowanie regulujących je mechanizmów homeostatycznych, natomiast różnice w stężeniu magnezu wskazują na silny wpływ badanych czynników na jego metabolizm. Różnice w wartości wskaźnika hematokrytowego, stężenie wapnia i magnezu w pełnej krwi oraz brak zmiany stężenia w osoczu wskazują na przesunięcie badanych pierwiastków do erytrocytów, co może świadczyć o destabilizacji błony komórkowej oraz wzroście jej przepuszczalności.

Słowa kluczowe

EN

food supplementation; B-group vitamin; calcium metabolism; magnesium metabolism; diet; rat; diet composition

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Technologia Alimentaria
• Rocznik: 2010
• Tom: 09
• Numer: 3
• Opis fizyczny: p.363-372,ref.

Twórcy

autor

Sadowska J.

• West Pomeranian University of Technology in Szczecin, Papieza Pawla VI 3, 71-459 Szczecin, Poland

Bibliografia

• Arunabh S., Pollack S., Yeh J., Aloia J.F., 2003. Body fat content and 25-hydroxyvitamin D levels in healthy women. J. Clin. Endorinol. Metab. 88, 157-161.
• Carpenter J.W., Mashima T.Y., Rupiper D.J., 2001. Exotic animal formulary. W.B. Saunders Company Philadelphia.
• Czerwińska E., Walicka M., Talalaj M., Marcinkowska-Suchowierska E., Lisiak W., Wierzbicki Z., Rowiński W., 2004. Bone mass in women with morbid obesity. Int. J. Obes. 28 (Supl. SI), 116.
• Dargelos E., Brulé C., Stuelsatz P., Mouly V., Veschambre P., Cottin P., Poussard S., 2010. Up-regulation of calcium-dependent proteolysis in human myoblasts under acute oxidative stress. Exp. Cell. Res. 316, 115-125.
• Friedrich M., Dolot A., 2009. Assessment of effects of diet composition and vitamin B supplementation on free radical-related process in the body. Contents of non-enzymatic components of antioxidation defence and lipid peroxidation products in rat tissues. Pol. J. Food Nutr. Sci. 3,255-263.
• Friedrich M., Sadowska J., 2005. Effects of diet supplementation with B-complex vitamins on fatty tissue accumulation in rats. Pol. J. Food Nutr. Sci. 14/55, 189-194.
• Friedrich M., Sadowska J., Goluch-Koniuszy Z., 2009. The estimation of the effect of diet composition and its supplementation with B vitamins on the level of insulin and chosen indicators of protein transmutation at female rats. Food. Sci. Technol. Qual. 4, 361-367.
• Friedrich M., Sadowska J., Sawicka A., 2005. The effect of supplementing the diet with B vitamins on the composition of fatty acids in the fat tissue of peri-organs and on the process of fatty acid peroxidation in rat. Food. Sci. Technol. Qual. 45, 139-150.
• Gey K.F., Puska P., Jordan P., Moser U.K., 1991. Inverse correlation between plasma vitamin E and mortality from ischaemic heart disease in cross-cultural epidemiology. Am. J. Clin. Nutr. 53 (suppl 1), 326-345.
• Gey K.F., Stahelin H.B., Eichholzer M., 1993. Poor plasma status of carotene and vitamin C is associated with higher mortality from ischemic heart disease and stroke. Basel Prospec. Stud. Clin. Investig. 71, 3-6.
• Hofmanova J., Zadak Z., Hyspler R., Mikeska J., Zdansky P., Vaculova A., Neticova J., Kozubik A., 2005. The effects of parenteral lipid emulsions on cancer and normal human colon epithelial cells in vitro. Physiol. Res. 54, 409-418.
• Izzoti A., Bagnis A., Sacca S.C., 2006. The role of oxidative stress in glaucoma. Mutat. Res. 612, 105-114.
• Kremer R., Campbell P.P., Reinhardt T., Gilsanz V., 2009. Vitamin D status and its relationship to body fat, final height, and peak bone mass in young women. J. Clin. Endocrinol. Metab. 94, 67-73.
• Malon A., Brockmann C., Fijalkowska-Morawska J., Rob P., Maj-Zurawska M., 2004. Ionized magnesium in erythrocytes - the best magnesium parameter to observe hypo- or hypermagnesemia. Clin. Chim. Acta 349, 67-73.
• Monninkhof E.M., Velthuis M.J., Peeters P.H., Twisk J.W., Schuit A.J., 2009. Effect of exercise on postmenopausal sex hormone levels and role of body fat: a randomized controlled trial. J. Clin. Oncol. 27, 4492-4499.
• Olusi S.O., 2002. Obesity is an independent risk factor for plasma lipid peroxidation and depletion of erythrocyte cytoprotectic enzymes in humans. Int. J. Obes Relat. Metab. Disord. 26, 1159-1164.
• Omenn G.S., Goodman G.E., Thornquist M.D., Balmes J., Cullen M.R., Glass A., Keogh J.P., Meyskens F.L., Valanis B., Williams J.H., Barnhart S., Hammar S., 1996. Effects of a combination of P-carotene and vitamin A on lung cancer and cardiovascular disease. N. Engl. J. Med. 334, 1150-1155.
• Pasquali R., Vicennati V., Bertazzo D., Casimirri F., Pascal G., Tortelli O., Labate A.M., 1997. Determinants of sex hormone-binding globulin blood concentrations in premenopausal and postmenopausal women with different estrogen status. Virgilio-Menopause-Health Group. Metabolism 46, 5-9.
• Reeves P.G., Nielsen F.H., Fahey G.C., 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76 rodent diet. J. Nutr. 123, 1939-1951.
• Saab G., Whaley-Connell A., McFarlane S.I., Li S., Chen S.C., Sowers J.R., McCullough P.A., Bakris G.L., Kidney Early Evaluation Program Investigators, 2010. Obesity is associated with increased parathyroid hormone levels independent of glomerular filtration rate in chronic kidney disease. Metabolism 59, 385-389.
• Sadowska J., Serwotka J., 2001. Effect of controlled vitamin supplementation on the levels of chosen components and enzymes of protein transmutation in blood and rats tissues. Folia Univ. Agric. Stetin. 220, Scient. Aliment. 1, 65-72.
• Śliwa E., Studziński T., Tatara M.R, 2006. Glikokortykosteroidy a metabolizm i wzrost kości [Glucocorticoids and the metabolism and skeletal system growth]. Med. Wet. 62, 377-379 [in Polish].
• StatSoft 2009. STATISTICA (data analysis software system). Version 9.0. www.statsoft.com.
• Tremollieres F.A., Pouilles J.M., Ribot C., 1993. Vertebral postmenopausal bone loss is reduced in overweight women: a longitudinal study in 155 early postmenopausal women. J. Clin. Endocrinol. Metab. 77, 683-686.
• Verlangieri A.J., Kapeghian J.C., el-Dean S., Bush M., 1985. Fruit and vegetable consumption and cardiovascular mortality. Med. Hypotheses 16, 7-15.
• Virtamo J., Rapola J., Ripatti S., Heinonen O.P., Taylor P.R., Albanes D., Huttunen J.K., 1998. Effect of vitamin E and β-carotene on the incidence of primary nonfatal myocardial infarction and fatal coronary heart disease. Arch. Intern. Med. 158, 668-675.
• Zallone A., 2006. Direct and indirect estrogen actions on osteoblasts and osteoclasts. Ann. N.Y. Acad. Sci. 1068, 173-179.
• Zemel M.B., 1995. Insulin resistance vs. hyperinsulinemia in hypertension: insulin regulation of Ca2+ transport and Ca2+ regulation of insulin sensitivity. J. Nutr. 125, 1738S-1743S.
• Zemel M.B., Shi H., Greer B., Dirienzo D., Zemel P.C., 2000. Regulation of adiposity by dietary calcium. FASEB J. 4, 1132-1138.