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Czasopismo

2008 | 60 |

Tytuł artykułu

Structure of embryogenic tissues and accumulation of storage materials in somatic embryos of Picea abies and P. omorika

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Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Microscopic analysis of embryogenic tissues of Picea abies and P. omorika showed that the tissues differed in proportions of various types of proembryogenic masses, and in presence/absence of early somatic embryos, which suggests that they differed in embryogenic potential. On the basis of this study, embryogenic competence of tissues can be assessed at early stages of somatic embryogenesis, and thus the most efficient lines can be selected (one Picea abies and two P. omorika). In the course of development of somatic embryos embryogenic tissue was placed on a medium with abscisic acid (ABA), which affects an increase in osmotic pressure at the stage of maturation of somatic embryos and finally on the regeneration of cotyledonary embryos. In this stage of embryos development, storage materials were accumulated, which are used in specific metabolic processes during embryo development.

Wydawca

-

Czasopismo

Rocznik

Tom

60

Opis fizyczny

p.19-28,fig.,ref.

Twórcy

  • Polish Academy of Sciences, Institute of Dendrology, Parkowa 5, 62-035 Kornik, Poland
autor
autor

Bibliografia

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  • Egertsdotter U., von Arnold S. 1993. Classification of embryogenic cell-lines of Picea abies as regards protoplast isolation and culture. Journal of Plant Physiology 141: 222–229.
  • Egertsdotter U., von Arnold S. 1995. Importance of arabinogalactan proteins for the development of somatic embryos of Norway spruce (Picea abies). Physiologia Plantarum 93: 334–345.
  • Egertsdotter U., von Arnold S. 1998. Development of somatic embryos in Norway spruce. Journal of Experimental Botany 49: 155–162.
  • Filonova L. H., Bozhkov P.V., von Arnold S. 2000. Developmental pathway of somatic embryogenesis in Picea abies as revealed by time-lapse tracking. Journal of Experimental Botany 51: 249–264.
  • Flinn B.S., Roberts D.R., Taylor I.E.P. 1991a. Evaluation of somatic embryos of interior spruce: Characterisation and developmental regulation of storage proteins. Physiologia Plantarum 82: 624–632.
  • Flinn B.S., Roberts D.R., Webb D.T., Sutton B.C.S. 1991b. Storage protein changes during zygotic embryogenesis in interior spruce. Tree Physiology 8: 71–81.
  • Flinn B.S., Roberts D.R., Newton C.H., Cyr D.R., Webster F.B., Taylor E.P. 1993. Storage protein gene expression in zygotic and somatic embryos of interior spruce. Physiologia Plantarum 89: 719–730.
  • Gupta P.K., Durzan D.J. 1986. Plantlet regeneration via somatic embryogenesis from subcultured callus of mature embryos of Picea abies (Norway spruce). In Vitro Cellular and Developmental Biology – Plant 22: 685–688.
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Typ dokumentu

Bibliografia

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