The involvement of protein kinase A in the immune response of Galleria mellonella larvae to bacteria

• Autorzy: Cytrynska M. , Zdybicka-Barabas A. , Jakubowicz T.
• Języki publikacji: EN

Abstrakty

EN

The role of protein kinase A (PKA) in the humoral immune response of the greater wax moth Galleria mellonella larvae to live Gram-positive bacteria Micrococcus lysodeikticus and Gram-negative bacteria Escherichia coli was investigated. The immune challenge of larvae with both kinds of bacteria caused an increase in fat body PKA activity depending on the injected bacteria. Gram-positive M. lysodeikticus was a much better inducer of the enzyme activity than Gram-negative E. coli. The PKA activity was increased about 2.5-fold and 1.5-fold, after M. lysodeikticus and E. coli injection, respectively. The in vivo inhibition of the enzyme activity by a cell permeable selective PKA inhibitor, Rp-8-Br-cAMPS, was correlated with considerable changes of fat body lysozyme content and hemolymph antimicrobial activity in bacteria-challenged insects. The kinetics of changes were different and dependent on the bacteria used for the immune challenge of G. mellonella larvae.

Słowa kluczowe

EN

Galleria mellonella; Gram-negative bacterium; greater wax moth; Micrococcus lysodeikticus; Gram-positive bacterium; lysozyme; antibacterial activity; Escherichia coli; protein kinase A; larva; immune response

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2007
• Tom: 54
• Numer: 1
• Opis fizyczny: p.167-174,fig.,ref.

Twórcy

autor

Cytrynska M.

• Maria Sklodowska-Curie University, Lublin, Poland

autor

Zdybicka-Barabas A.

autor

Jakubowicz T.

Bibliografia

• Boman HG, Nilsson-Faye I, Paul K, Rasmuson T Jr (1974) Insect immunity. 1. Characteristics of an inducible cellfree antibacterial reaction in hemolymph of Samia cynthia pupae. Infect Immun 10: 136–145.
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.
• Briggs LJ, Stein D, Goltz J, Corrigan VC, Efthymiadis A, Hübner S, Jans DA (1998) The cAMP-dependent protein kinase site (Ser312) enhances Dorsal nuclear import through facilitating nuclear localization sequence/importin interaction. J Biol Chem 273: 22745–22752.
• Brooks CL, Dunphy GB (2005) Protein kinase A affects Galleria mellonella (Insecta: Lepidoptera) larval haemocyte non-self responses. Immunol Cell Biol 83: 150–159.
• Chadwick JS, Aston WP (1991) Antibacterial immunity in Lepidoptera. In Immunology of insects and other arthro pods. Gupta AP, ed, pp 347–370. CRC Press, Boca Raton, Ann Arbor, London.
• Chalk R, Suliaman WY (1998) Antimicrobial peptides from small insects: methods for insect culture and for the detection, visualization, isolation and sequencing of active hemolymph peptides. In Techniques in insect immunology. Wiesner A, Dunphy GB, Marmaras VJ, Morishima I, Sugumaran M, Yamakawa M, eds, pp 109–124. SOS Publications, USA.
• Chung KT, Ourth DD (2000) Larval and pupal induction and N-terminal amino acid sequence of lysozyme from Heliothis virescens. J Insect Physiol 46: 563–572.
• Cytryńska M, Zdybicka-Barabas A, Jabłoński P, Jakubowicz T (2001) Detection of antibacterial polypeptide activity in situ after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem 299: 274–276.
• Cytryńska M, Zdybicka-Barabas A, Jakubowicz T (2006) Studies on the role of protein kinase A in humoral immune response of Galleria mellonella larvae. J Insect Physiol 52: 744–753.
• Dickinson L, Russell V, Dunn PE (1988) A family of bacteria- regulated, cecropin D-like peptides from Manduca sexta. J Biol Chem 263: 19424–19429.
• Dunn PE, Dai W, Kanost MR, Geng CX (1985) Soluble peptidoglycan fragments stimulate antibacterial protein synthesis by fat body from larvae of Manduca sexta. Dev Comp Immunol 9: 559–568.
• Hoffmann D, Hultmark D, Boman HG (1981) Insect immunity: Galleria mellonella and other Lepidoptera have cecropia-P9-like factors active against Gram negative bacteria. Insect Biochem 11: 537–548.
• Hultmark D (1996) Insect lysozymes. In Lysozymes: model enzymes in biochemistry and biology. Jollés P, ed, pp 87–102. Birkhäuser Verlag Basel/Switzerland.
• Hultmark D (2003) Drosophila immunity: paths and patterns. Curr Opin Immunol 15: 12–19.
• Hultmark D, Engström A, Bennich H, Kapur R, Boman HG (1982) Insect immunity: Isolation and structure of cecropin D and four minor antibacterial components from Cecropia pupae. Eur J Biochem 127: 207–217.
• Iwanaga S, Lee BL (2005) Recent advances in the innate immunity of invertebrate animals. J Biochem Mol Biol 38: 128–150.
• Kanost MR, Dai W, Dunn PE (1988) Peptidoglycan fragments elicit antibacterial protein synthesis in larvae of Manduca sexta. Arch Insect Biochem Physiol 8: 147–164.
• Kurata S (2004) Recognition of infectious non-self and activation of immune responses by peptidoglycan recognition protein (PGRP) — family members in Drosophila. Dev Comp Immunol 28: 89–95.
• Kurata S, Ariki S, Kawabata S-J (2006) Recognition of pathogens and activation of immune responses in Drosophila and horseshoe crab innate immunity. Immunobiology 211: 237–249.
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277: 680–685.
• Lavine MD, Chen G, Strand MR (2005) Immune challenge differentially affects transcript abundance of three antimicrobial peptides in hemocytes from the moth Pseudoplusia includens. Insect Biochem Mol Biol 35: 1335–1346.
• Leclerc V, Reichhart J-M (2004) The immune response of Drosophila melanogaster. Immunol Rev 198: 59–71.
• Leulier F, Parquet C, Pili-Floury S, Ryn JH, Caroff M, Lee WJ, Mengin-Lecreulx D, Lemaitre B (2003) The Drosophila immune system detects bacteria through specific peptidoglycan recognition. Nat Immunol 4: 478–484.
• Lowenberger C (2001) Innate immune response of Aedes aegypti. Insect Biochem Mol Biol 31: 219–229.
• Mak P, Chmiel D, Gacek GJ (2001) Antibacterial peptides of the moth Galleria mellonella. Acta Biochim Polon 48: 1191–1195.
• Marin D, Dunphy GB, Mandato CA (2005) Cyclic AMP affects the haemocyte responses of larval Galleria mellonella to selected antigens. J Insect Physiol 51: 575–586.
• Morishima I, Horiba T, Iketani M, Nishioka E, Yamano Y (1995) Parallel induction of cecropin and lysozyme in larvae of the silkworm Bombyx mori. Dev Comp Immunol 19: 357–363.
• Norris JL, Manley JL (1992) Selective nuclear transport of the Drosophila morphogen dorsal can be established by a signaling pathway involving the transmembrane protein Toll and protein kinase A. Genes Dev 6: 1654–1667.
• Pinheiro VB, Ellar DJ (2006) How to kill a mocking bug? Cell Microbiol 8: 545–557.
• Powning RF, Davidson WJ (1973) Studies on insect bacteriolytic enzymes. I. Lysozyme in haemolymph of Galleria mellonella and Bombyx mori. Comp Biochem Physiol B 45: 669–686.
• Royet J, Reichhart J-M, Hoffmann JA (2005) Sensing and signalling during infection in Drosophila. Curr Opin Immunol 17: 11–17.
• Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368–379.
• Schoofs L, Holman GM, Hayes TK, Nachman RJ, De Loof A (1990) Locusta tachykinin I and II, two novel insect neuropeptides with homology to peptides from the vertebrate tachykinin family. FEBS Lett 261: 397–401.
• Shimabukuro M, Xu J, Sugiyama M, Taniai K, Kadono- Okuda K, Kato Y, Yamamoto M, Chowdhury S, Choi SK, Choi HK, Miyanoshita A, Debnath NC, Yamakawa M (1996) Signal transduction for cecropin B gene expression in adherent hemocytes of the silkworm, Bombyx mori (Lepidoptera: Bombycidae). Appl Entomol Zool 31: 135–143.
• Sun S-C, Asling B, Faye I (1991) Organization and expression of the immunoresponsive lysozyme gene in the giant silk moth, Hyalophora cecropia. J Biol Chem 266: 6644–6649.
• Taniai K, Furukawa S, Shono T, Yamakawa M (1996) Elicitors triggering the simultaneous gene expression of antibacterial proteins of the silkworm, Bombyx mori. Biochem Biophys Res Commun 226: 783–790.
• Tanji T, Ip YT (2005) Regulators of the Toll and Imd pathways in the Drosophila innate immune response. Trends Immunol 26: 193–198.
• Vilcinskas A, Matha V (1997) Effect of the entomopathogenic fungus Beauveria bassiana on the humoral immune response of Galleria mellonella larvae (Lepidoptera: Pyralidae). Eur J Entomol 94: 461–472.
• Wang L, Ligoxygakis P (2006) Pathogen recognition and signalling in the Drosophila innate immune response. Immunobiology 211: 251–261.
• Wojda I, Kowalski P, Jakubowicz T (2004) JNK MAP kinase is involved in the humoral immune response of the greater wax moth larvae Galleria mellonella. Arch Insect Biochem Physiol 56: 143–154.
• Yamakawa M, Tanaka H (1999) Immune proteins and their gene expression in the silkworm, Bombyx mori. Dev Comp Immunol 23: 281–289.
• Zakarian RJ, Dunphy GB, Rau ME, Albert PJ (2003) Kinases, intracellular calcium, and apolipophorin-III influence the adhesion of larval hemocytes of the lepidopterous insect, Galleria mellonella. Arch Insect Biochem Physiol 53: 158–171.