Preliminary investigations of relative polarity of dissolved humic substances in select lakes of the Elk District, Poland

• Autorzy: Glazewski R , Wojcik I
• Języki publikacji: EN

Abstrakty

EN

The reported study describes the separation of humic substances (HS) dissolved in surface waters into hydrophilic and hydrophobic fractions with the SPE method onto StrataX (Phenomenex) columns. The separated fractions were analyzed with the HPLC-C18 (Merck column) method for relative polarity of humic (HA) and fulvic (FA) acids in the samples. Analyses were carried out on samples collected from 7 lakes constituting two cascade systems located in the same catchment of the Ełk River. During HPLC assays, an effect of size exclusion (SE) was observed, especially for the hydrophilic fraction. The HPLC analyses enabled us to demonstrate that in both hydrological systems the hydrophilic fraction contained mainly molecules with molecular weights exceeding 1,000Da and of practically equal relative polarity. Only in system II (five lakes) was the hydrophobic fraction strongly diversified quantitatively as well as in terms of molecular weight and polarity between the samples. Results of analysis show that this system was subject to a strong anthropogenic impact.

Słowa kluczowe

EN

Elckie Lakeland; Poland; lake; dissolved humic substance; lake ecosystem; natural organic matter; humic substance; functionality; relative polarity; surface water; natural humic substance

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2009
• Tom: 18
• Numer: 4
• Opis fizyczny: p.599-606,fig.,ref

Twórcy

autor

Glazewski R

• University of Warmia and Mazury, Prawochenskiego 1/38, 10-795 Olsztyn, Poland

autor

Wojcik I

• University of Humanities and Life Sciences, Swietokrzyska 15/115A, 25-406 Kielce, Poland

Bibliografia

• 1. GÓRNIAK A. Humic substances and its role in functioning of freshwater ecosystems. Dissertationes Universitatis Varsoviensis 488, Białystok, 1996, [In Polish].
• 2. McKNIGHT D.M., AIKEN G.R. Sources and Age of Aquatic Humus, [in:] Hessen D.O., Tranvik L.J., (Eds), Aquatic Humic Substances. Ecology and Biogeochemistry, Springer-Verlag, Berlin, 346, 9, 1998.
• 3. GERGEL S.E., TURNER M.G. KRATZ T.K. Dissolved organic carbon as an indicator of the scale of watershed influence on lakes and rivers. Ecological Appl., 9(4), 1377, 1999.
• 4. CHEN J., GU B., LeBOEUF E.J., PAN H., DAI S. Spectroscopic characterization of the structural and functional properties of natural organic matter fractions. Chemosphere 48, 59, 2002.
• 5. McDONALD S., BISHOP A.G., PRENZLER P.D., ROBARDS K. Analytical chemistry of freshwater humic substances. Analytica Chimica Acta 527, 105, 2004.
• 6. WARNKEN K.W., SANTSCHI P.H. Biogeochemical behavior of organic carbon in the Trinity River downstream of a large reservoir lake in Texas, USA. Sci. Tot Eviron., 329, 131, 2004.
• 7. JANOŠ P. Separation methods in the chemistry of humic substances, J. Chromatogr., A, 983, 1, 2003.
• 8. LEPANE V. Comparison of XAD resin for the isolation of humic substances from seawater, J. Chromatogr., A, 845, 329, 1999.
• 9. PEURAVUORI J., PAASO N., PIHLAJA K. Sorption behaviour of some chlorophenols in lake aquatic humic matter. Talanta 56, 523, 2002.
• 10. SAKKAS V., LAMBROPOULOU D., ALBANIS T. Photochemical degradation study of irgarol 1051 in natural waters: influence of humic and fulvic substances on the reaction. Journal of Photochemistry and Photobiology A: Chemistry 147, 135, 2002.
• 11. KUBICKI J.D., APITZ S.E. Models of natural organic matter and interactions with organic contaminants. Organic Geochemistry 30, 911, 1999.
• 12. XUE H., SIGG L. Comparison of the Complexation of Cu and Cd by Humic or FulvicAcids and by Ligands Observed in Lake Waters. Aquatic Geochemistry 5, 313, 1999.
• 13. KUBICKI J.D., TROUT C.C. Molecular Modeling of Fulvic and Humic Acids: Charging Effect and Interaction with Al+3, Benzene and Pyridine, [in:] Selim H.M., Kingery W., (Eds), Geochemical and Hydrological Reactivity of Heavy Metals in Soils, CRC-Taylor and Francis, pp. 113-143, 2003.
• 14. TROUT C.C., KUBICKI J.D. Molecular modeling of Al+3 and benzene interactions with Sawannee fulvic acid. Geochim. Cosmochim. Acta 71, 3859, 2007.
• 15. FRIMMEL F.H. Characterization of natural organic matter as major constituents in aquatic systems. Journal of Contaminant Hydrology 35, 201, 1998.
• 16. IMAI A., FUKUSHIMA T., MATSUSHIGE K., KIM Y.H. Fractionation and characterization of dissolved organic matter in a shallow eutrophic lake, its inflowing rivers, and other organic matter sources. Water Research 35, (17), 4019, 2001.
• 17. SCOTT M.J., JONES M.N., WOOF C., SIMON B., TIPPING E. The molecular properties of humic substances isolated from a UK upland peat system: A temporal investigation, Environment Int., 27(6), 449, 2001.
• 18. MORAN M.A., ZEPP R.G. Role of photoreactions in the formation of biologically labile ompounds from dissolved organic matter. Limnol. Oceanogr., 42(6), 1307, 1997.
• 19. PEURAVUORI J., PIHLAJA K. Multimethod characterization of lake aquatic humic matter isolated with sorbing solid and tangential membrane filtration. Anal. Chim. Acta 364, 203, 1998.
• 20. KIM H-C., YU M-J., HAN I. Multi-method study of characteristics chemical nature of aquatic humic substances isolated from the Han River, Korea. Applied Geochemistry 21, 1226, 2006.
• 21. KIRKPATRICK G.J., ORRICO C., MOLINE M.A., OLIVER M., SCHOFIELD O.M. Continuous hyperspectral absorption measurements of colored dissolved organic material in aquatic systems, Applied Optics 42, (33), 6564, 2003.
• 22. ALVAREZ-PUEBLA R.A., GOULET P.J.G , GARRIDO J.J. Characterization of the porous structure of different humic fractions. Colloids and Surfaces A: Physicochem. Eng. Aspects 256, 129, 2005.
• 23. GŁAŻEWSKI R., PERSZUTO K. The introductory estimation of the selected lakes purity in the Ełk district. [in:] Kistowski M., Masdorf J. (Eds), The resources and threats to the natural environment in the Ełk district and in Niemenczyn. Report, pp. 35-54, 2004.
• 24. LANGE W., MAŚLANKA W., NOWICKI K. Susceptibility to anthropopressure and intensity of eutrophication of chosen lakes of Ełk Lakeland. Limnological Review 4, 133, 2004
• 25. JAŃCZAK J., MAŚLANKA W. Cases of occurrence of secondary metalimnia in some lakes of the Ełk Lakeland. Limnological Review 6, 123, 2006.
• 26. ŚWIETLIK J., SIKORSKA I. Characterization of Natural Organic Matter Fractions by High Pressure Size-Exclusion Chromatography, Specific UV Absorbance and Total Luminescence Spectroscopy. Pol. J. of Enviro. Stud. 15, (1), 145, 2005.
• 27. PEURAVUORI J., PIHLAJA K., VALIMAKI N. Isolation and characterization of natural organic matter from lake water: two different adsorption chromatographic methods. Environ. Int. 23(4), 453, 1997.
• 28. CURTIS M.A., WITT A.F., SCHRAM S.B., ROGERS L.B. Humic acid fractionaction using a nearly linear pH gradient, Anal. Chem., 53, 1195, 1981.
• 29. OHGA K., ARITOMI Y., OHTSU H. Chromatography with pH-gradient elution of dissolved humic substances in river water, Anal. Sci., 5, 215, 1989.
• 30. DITTMAR T., KOCH B., HERTKORN N., KATTNER G. A simple and efficient method for the solid-phase extraction of dissolved organic matter (SPE-DOM) from seawater. Limnol. Oceanogr.:Methods 6, 230, 2008.
• 31. ROUBEUF V., MOUNIER S., BENAIM J.Y. Solid phase extraction applied to natural waters: efficiency and selectivity, Organic Geochemistry 31, 127, 2000.
• 32. FONTANALS N., MARCE R.M., BORRULL F. New hydrophilic materials for solid-phase extraction. Trends in Analytical Chemistry, 24, (5), 395, 2005.
• 33. WOELKI G., FRIEDRICH S., HANSCHMANN G., SALZER R. HPLC fractionation and structural dynamics of humic acids. Fresenius J Anal Chem 357, 548, 1997.
• 34. GŁAŻEWSKI R., WÓJCIK I. Preliminary examinations of the functional properties of dissolved organic matter in selected lakes of the district of Ełk, Limnological Review 7(2), 35, 2007.
• 35. WETZEL R.G., HATCHER P.G., BIANCHI T.S. Natural photolysis by ultraviolet irradiance of recalcitrant dissolved organic matter to simple substrates for rapid bacterial metabolism. Limnol. Oceanogr., 40(8), 1369, 1995.
• 36. ABBT-BRAUN G., LANKES U., FRIMMEL F. Structural characterization of aquatic humic substances- The need for a multiple method approach, Aquat. Sci. 66, 151, 2004.
• 37. ALBERTS J.J., TAKAS M. Comparison of the natural fluorescence distribution among size fractionations of terrestrial fulvic and humic acids and aquatic natural organic matter, Organic Geochemistry, 35, 1141, 2004.
• 38. SIERRA M.M.D., GIOVANELA M., PARLANTI E., SORIANO-SIERRA E.J. 3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids, J.Braz.Chem.Soc., 17(1), 113, 2006.
• 39. SZABO L.P. Characterization of alginite humic acid content, Desalination, 163, 85, 2004.
• 40. WU F.C., EVANS R.D., DILLON P.J., CAI Y.R. Rapid quantification of humic and fulvic by HPLC in natural waters, Applied Geochemistry, 22, 1598, 2007.
• 41. GÓRNIAK A., WOJCIECHOWSKI I. Humic substances in the polihumic lake ecosystem in selected basin of Nothestern Poland. Abstracts 4-th Nordic Symp. on Humus Substances and Humex Project Seminar, Loen, Norway, 2, 2003.
• 42. KAISER E., ARSCOTT D.B., TOCKNER K., SULZBERG B. Sources and distribution of organic carbon and nitrogen in the Tagliamento River, Italy. Aquat. Sci., 66, 103, 2004.
• 43. BATTIN T.I. Dissolved organic matter and its optical properties in a blackwater tributary on the upper Orinoco river, Venezuela. Org.Geochem., 28, (9/10), 561, 1998.
• 44. DeHAAN H. Solar UV-light penetration and photodegradation of humic substances in peaty lake water. Limnol. Oceanogr., 38(5), 1072, 1993.