Effect of disulfide and sulphydryl reagents on abortive and productive elongation catalyzed by Escherichia coli RNA polymerase

• Autorzy: Radlowski M. , Job D.
• Języki publikacji: EN

Abstrakty

The effect of disulfide and sulfhydryl reagents on the rate of abortive and productive elongation has been studied using Escherichia coli RNA polymerase holoenzyme and poly[d(A-T)] as template. In the presence of UTP as a single substrate and UpA as a primer, the enzyme catalyzed efficiently the synthesis of the trinucleotide product UpApU. Incubation of RNA polymerase with 1 mM 2-mercaptoethanoi resulted in a 5-fold increase of the rate of UpApU synthesis. In contrast, incubation of the enzyme with 1 mM 5,5'-dithio-bis(2-nitrobenzoic) acid resulted in a 6-fold decrease of the rate of abortive elongation. Determination of the steady state kinetic constants associated with UpApU synthesis disclosed that the disulfide and sulfhydryl reagents mainly affected the rate of UpApU release from the ternary transcription complexes and therefore influenced the stability of such complexes.

Słowa kluczowe

EN

disulphide reagent; RNA; productive elongation; Escherichia coli; abortive elongation; RNA polymerase; sulphydryl reagent; elongation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1994
• Tom: 41
• Numer: 4
• Opis fizyczny: p.415-419,fig.

Twórcy

autor

Radlowski M.

• Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Job D.

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