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1996 | 43 | 2 |

Tytuł artykułu

Interactions of rat repetitive sequence MspI8 with nuclear matrix proteins during spermatogenesis

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Using the Southwestern blot analysis we have studied the interactions between rat repetitive sequence MspI8 and the nuclear matrix proteins of rat testis cells. Starting from 2 weeks the young to adult animals showed differences in type of testis nuclear matrix proteins recognizing the MspI8 sequence. The same sets of nuclear matrix proteins were detected in some fractions enriched in spermatocytes and spermatides and obtained after fractionation of testis cells of aduit animals by the velocity sedimentation technique.

Wydawca

-

Rocznik

Tom

43

Numer

2

Opis fizyczny

p.319-324,fig.

Twórcy

  • Institute of Oncology, 44-100 Gliwice, Poland
autor

Bibliografia

  • 1. Gasser. S.M., Amati, B.B., Cardenas, M.E. & Hoffmann, J.F.-X. (1989) Studies on scaffold attachment sites and their relation to genome function. Int. Rev. Cytol. 119,57-%.
  • 2. Cockerill, P.N. & Garrard, W.T. (1986) Chromo­somal loop anchorage of the kappa immuno­globulin gene occurs next to the enhancer in a region containing topoisomerase II sites. Cell 44, 273-282.
  • 3. Boulikas, T. (1993) Nature of DNA sequences at the attachment regions of genes to the nuclear matrix. J. Cell. Biochem. 52,14-22.
  • 4. Cockerill, P.N., Yuen, M.H. & Garrard, W.T. (1987) The enhancer of the immunoglobulin heavy chain locus is flanked by presumptive chromosomal loop anchorage elements. J. Biol. Chem. 262,5394-5397.
  • 5. Farache, G., Razin, S.V., Rzeszowska-Wolny, J., Moreau, J., Recillas Targa, F. & Scherrer, K. (1990) Mapping of structural and transcription-related matrix attachment sites in the a-globin gene domain of avian erythroblasts and erythrocytes. Mol. Cell. Biol. 10,5349-5358.
  • 6. Gerdes, M.G., Carter, K.C., Moen, P.T., Jr. & Lawrence, J.B. (1994) Dynamic changes in the higher-level chromatin organization of specific sequences revealed by in situ hybridization to nuclear haios. J. Cell Biol. 126,289-304.
  • 7. Rzeszowska-Wolny, J. & Rogolinski, J. (1994) MspI8, the repetitive sequence specifically interacting with nuclear matrix of rat testis cells. Acta Biochim. Polon. 41,459^71.
  • 8. Molecular Biology of the Male Reproductive System (1993) (de Kretser, D., ed.) Academic Press Inc., New York.
  • 9. Ward, W.S., Partin, A.W. & Coffey, D.S. (1989) DNA loop domains in mammalian sperma­tozoa. Chromosoma 98,153-159.
  • 10. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual; 2nd edn., Cold Spring Harbor Laboratory Press.
  • 11. Berezney, R. & Coffey, DS. (1974) Identification of nuclear protein matrix. Biochem. Biophys. Res. Commun. 60,1410-1417.
  • 12. Wolgemuth, D.J., Gizang-Ginsberg, E., Enge- Imyer, E., Gavin, B.J. & Ponzetto, C. (1985) Separation of mouse testis cells on a Cclsep apparatus and their usefulness as a source of high molecular weight DNA or RNA. Gamete Res. 12,1-10.
  • 13. Meistrich, M.L. (1977) Separation of sperma- togenic cells and nuclei from rodent testes. Meth. Cell Biol. 15,15-54.
  • 14. Bellve', A.R. (1993) Purification, culture and fractionation of spermatogenic cells. Meth. F.nzymol. 225, 84-113.
  • 15. Lam, D.M., Furrer, R. & Bruce, W.R. (1970) The separation, physical characterization and diffe­rentiation kinetics of spermatogonial cells of the mouse. Proc. Natl. Acad. Sci. U.S.A. 65,192-199.
  • 16. Mirkovitch, J., Gasser, S.M. & Laemmli, U.K. (1988) Scaffold attachment of DNA loops in metaphase chromosomes. J. Mol. Biol. 200, 101-109.
  • 17. Rzeszowska-Wolny, J., Razin, S., Puvion, E., Moreau, J. & Scherrer, K. (1988) Isolation and characterization of stable nuclear matrix prepa­rations and associated DNA from avian erythro­blasts. Biol. Cell 64,13-22.
  • 18. Ivanchenko, M. & Avramova, Z. (1992) Inte­raction of MAR-sequences with nuclear matrix proteins. /. Cell. Biochem. 50,190-200.
  • 19. Morimoto, R.I. (1993) Cell in stress: Trans­criptional activation of heat shock genes. Science 259,1409-1410.
  • 20. Ward, WS. (1994) The structure of the sleeping genome: Implication of sperm DNA organi­zation for somatic cells. J. Cell. Biochem. 55,77-82.
  • 21. Clermont, Y. & Perey, B. (1957) Quantitative study of the cell population of the seminiferous tubules in immature rats. Am. J. Anat. 100, 241-268.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-7849fa90-f5f2-482d-9258-094ad7b8e41e
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