Analysis of mtDNA sequences of European grayling, Thymallus thymallus, from Southwestern Poland

• Autorzy: Jurczyk L , Brzuzan P
• Języki publikacji: EN

Abstrakty

EN

Nucleotide sequences of the partial mitochondrial DNA (mtDNA) control region and flanking tRNA Phe gene (a total of 150bp) were determined in eight specimens of European grayling, Thymallus thymallus, representing five rivers in southwestern Poland. Two haplotypes, A and B, were identified which differed from each other by two nucleotide substitutions. Haplotype A was detected in fish from two rivers, the Zadrna and Bóbr, whereas haplotype B was detected in fish from three others, the Widna, Biala Gluchołazka and Metuja. The different phylogenetic position of the mtDNA haplotypes observed in samples from rivers which are in close proximity may suggest genetic structuring of grayling populations in the region, but further sampling is required to test this hypothesis.

PL

Analizowano sekwencje nukleotydowe o długości 150 par zasad, obejmujące fragment regionu regulacyjnego mtDNA 8 osobników lipienia europejskiego, Thymallus thymallus, reprezentujących stada z pięciu rzek południowo-zachodniej Polski (rys. 1). Wykryto obecność dwu typów (haplotypów) mtDNA („A" i „B"; tab. 2), różniących się od siebie dwoma podstawieniami w pozycjach 19 i 23 analizowanej sekwencji. Uzyskane wstępne wyniki badań wskazują na zróżnicowanie genetyczne lipieni w tym regionie Polski.

Słowa kluczowe

EN

Thymallus thymallus; mtDNA sequence; fish; mtDNA variation; mitochondrial DNA; haplotype; tRNA; Poland; phylogenetic position; European grayling; nucleotide sequence; grayling

• Wydawca: -
• Czasopismo: Archives of Polish Fisheries
• Rocznik: 2003
• Tom: 11
• Numer: 2
• Opis fizyczny: p.301-306,fig.,ref.

Twórcy

autor

Jurczyk L

• University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, Olsztyn, Poland

autor

Brzuzan P

• University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, Olsztyn, Poland

Bibliografia

• Bernatchez L., Guyomard R., Bonhomme F. 1992 - Sequence variation of the mitochondrial control region among geographically and morphologically remote European brown trout (Salmo trutta L.) populations - Mol. Ecol. 1: 161-173.
• Jurczyk L. 2001 - Phylogenetic connections between Arctic grayling Thymallus arcticus arcticus and Thymallus arcticus baicalensis based on the analysis of mitochondrial DNA sequences - M.Sc. dissertation, University of Warmia and Mazury, Olsztyn, Poland (in Polish).
• Koskinen M.T., Ranta E., Piironen J., Veselov A., Titov S., Haugen T.O., Nillson J., Carlstein M., Primmer C.R. 2000 - Genetic lineages and postglacial colonization of grayling (Thymallus thymallus, Salmonidae) in Europe, as revealed by mitochondrial DNA analyses - Mol. Ecol. 9: 1609-1624.
• Saitou N., Nei M. 1987 - The neigbour-joining method: a new method of reconstructing phylogenetic trees - Mol. Biol. Evol. 4: 406-425.
• Swofford D.L. 2000 - PAUP: Phylogenetic Analysis Using Parsimony (and Other Methods). Sinauer Associates, Sunderland.
• Weiss S., Persat H., Eppe R., Schloetterer C., Uibleins F. 2002 - Complex patterns of colonization and refugial revealed for European grayling Thymallus thymallus, based on compete sequencing of the mitochondrial DNA control region - Mol. Ecol. 11: 1393-1407.
• Witkowski A., Kowalewski M., Kokurewicz B. 1984 - European grayling - PWRiL, Warszawa (in Polish).