Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: control of an outbreak using a new ADSRRS technique

• Autorzy: Krawczyk B , Samet A. , Czarniak E. , Szczapa J. , Kur J.
• Języki publikacji: EN

Abstrakty

EN

Extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (EPKP) strains are frequently implicated in outbreaks in neonatal units. From April 2002 to January 2003, 149 neonates were colonized/infected with EPKP In the Neonatal Clinic of the Teaching Hospital at the Medical University of Gdansk, Poland. A novel assay based on suppression of PCR, ADSRRS-fingerprinting, was successfully evaluated for typing EPKP isolates. The results showed that the genotypes of all outbreak-related strains were identical, which suggested that the outbreak originated from a single clone. This conclusion was confirmed by using different methods - RAPD and PFGE. The outbreak was stopped by adopting improved hygiene and instituting outbreak control measures.

Słowa kluczowe

EN

random amplified polymorphic DNA; ADSRRS technique; extended-spectrum beta-lactamase; Klebsiella pneumoniae; microbiology; polymerase chain reaction; ADSRRS-fingerprinting

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2005
• Tom: 54
• Numer: 2
• Opis fizyczny: p.105-110,fig.,ref.

Twórcy

autor

Krawczyk B

• Gdansk University of Technology, G.Narutowicza 11/12, 80-952 Gdansk, Poland

autor

Samet A.

autor

Czarniak E.

autor

Szczapa J.

autor

Kur J.

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