A new method of DNA extraction from the ethanol-fixed parasitic worms

• Autorzy: Tkach V , Pawlowski J.
• Języki publikacji: EN

Abstrakty

EN

A protocol of the new method of DNA extraction from the ethanol fixed parasitic worms is described. The method is based on the use of the guanidine thiocyanate extraction buffer after evaporation of the ethanol from the tissues by drying. The method has been successfully used on several groups of parasitic Plathyhelminthes and provided higher DNA yield than a conventional phenol-chloroform extraction.

Słowa kluczowe

EN

evaporation; ethanol; phenol-chloroform extraction; parasitic worm; DNA extraction; guanidine thiocyanate; Platyhelminthes

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1999
• Tom: 44
• Numer: 2
• Opis fizyczny: p.147-148,fig.

Twórcy

autor

Tkach V

• Ukrainian Academy of Sciences, 15 Bogdan Khmelnitski Street, Kiev-30, MSP, 252601 Ukraine

autor

Pawlowski J.

Bibliografia

• Chomczynski P., Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162, 156-159.
• Hillis D. M., Moritz C., Mable B. K. 1996. Molecular systematics. Sinauer, Sunderland, MA, USA.
• Maniatis T., Fritsch E. F., Sambrook J. 1982. Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, NY, USA.