Transcriptional expression of a Solanum sogarandinum pGT::Dhn10 gene fusion in cucumber, and its correlation with chilling tolerance transgenic seedlings

• Autorzy: Yin Z , Pawlowicz I. , Bartoszewski G. , malinowski R. , Malepszy S. , Rorat T.
• Języki publikacji: EN

Abstrakty

EN

The expression pattern of a Solanum sogarandinum pGT::Dhn10 gene fusion encoding a dehydrin DHN10 protein and the potential role of that protein in cold tolerance in cucumber were analysed in three T1 transgenic lines. An accumulation of Dhn10 mRNA was detected in the leaves, cotyledons, hypocotyls and roots of the transgenic seedlings both under the control conditions and after a cold treatment at 6oC for 24 h. This was confirmed by RTPCR. However, no DHN10 protein was detected by the alkaline phosphataseconjugated antibody. The transgenic lines exhibited different levels of chilling tolerance. The TCC5/1 line showed a significant increase in its chilling tolerance compared to the non-transgenic line. No chilling injury was observed when the cold hardened (6oC, 24 h) TCC5/1 plants were subsequently exposed to a temperature of 2oC for 6 h. The other two transgenic lines, TCC2/1 and TCC3/2, exhibited a comparable level of chilling tolerance to that of the non-transgenic control. The transgenic lines showed similar or significantly decreased freezing tolerance compared to the non-transgenic control, as evaluated by an electrolyte leakage test. We concluded that the S. sogarandinum GT promoter is functional in the chilling sensitive species Cucumis sativus L., and that the pGT::Dhn10 gene fusion is expressed at the transcriptional level.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 4B
• Opis fizyczny: p.891-902,fig.,ref.

Twórcy

autor

Yin Z

• Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Pawlowicz I.

autor

Bartoszewski G.

autor

malinowski R.

autor

Malepszy S.

autor

Rorat T.

Bibliografia

• 1. Close, T.J. Dehydrins: A commonalty in the response of plants to dehydration and low temperature. Physiol. Plant. 100 (1997) 291-296.
• 2. Richard, S., Morency, M.J., Drevet, C., Jouanin, L. and Séguin, A. Isolation and characterization of a dehydrin gene from white spruce induced upon wounding, drought, and cold stresses. Plant Mol. Biol. 43 (2000) 1-10.
• 3. Campbell, S.A. and Close, T.J. Dehydrins: genes, proteins, and associations with phenotypic traits. New Phytol. 137 (1997) 61-74.
• 4. Egerton-Warburton, L.M., Balsamo, R.A. and Close, T.J. Temporal accumulation and ultrastructural localization of dehydrins in Zea mays. Physiol. Plant. 101 (1997) 545-555.
• 5. Bravo, L.A., Close, T.J., Corcuera, L.J. and Guy, C.L. Characterization of an 80-kDa dehydrin-like protein in barley responsive to cold acclimation. Physiol. Plant. 106 (1999) 177-183.
• 6. Nylander, M., Svensson, J., Palva, E.T. and Welin, B.V. Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana. Plant Mol. Biol. 45 (2001) 263-279.
• 7. Sarhan, F., Oullet, F. and Vazquez-Tello, A. The wheat wsc120 gene family: a useful model to understand the molecular genetics of freezing tolerance in cereals. Physiol. Plant. 101 (1997) 439-445.
• 8. Danyluk, J., Perron, A., Houde, M., Limin, A., Fowler, B., Benhamou, N. and Sarhan, F. Accumulation of an acidic dehydrin in the vicinity of the plasma membrane during cold acclimation of wheat. Plant Cell 10 (1998) 623-638.
• 9. Borovskii, G.B., Stupnikova, I.V., Antipina, A.A., Downs, C.A. and Voinikov, V.K. Accumulation of dehydrin-like protein in the mitochondria of cold-treated plants. J. Plant Physiol. 156 (2000) 797-800.
• 10. Heyen, B.J., Alsheikh, M.K., Smith, E.A., Torvik, C.F., Seals, D.F. and Randall, S.K. The calcium-binding activity of a vacuole-associated, dehydrin-like protein is regulated by phosphorylation. Plant Physiol. 130 (2002) 679-687.
• 11. Krüger, C., Berkowitz, O., Stephan, U.W. and Hell, R. A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L. J. Biol. Chem. 277 (2002) 2506225069.
• 12. Ismail, A.M., Hall, A.E. and Close, T.J. Allelic variation of a dehydrin gene cosegregation with chilling tolerance during seedling emergence. Proc. Natl. Acad. Sci. USA. 96 (1999) 13566-13570.
• 13. Palva, E.T. and Heino, P. Molecular mechanisms of plant cold acclimation and freezing tolerance. in: Plant Cold Hardiness: Molecular Biology, Biochemistry and Physiology, (Li, P.H. and Chen, T.H.H., Eds.), Plenum Press, New York, 1998, 1-14.
• 14. Whitsitt, M.S., Collins, R.G. and Mullet, J.E. Modulation of dehydration tolerance in soybean seedlings. Plant Physiol. 114 (1997) 917-925.
• 15. Cellier, F., Conéjéro, G., Breitler, J.C. and Casse, F. Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive lines of sunflower. Plant Physiol. 116 (1998) 319-328.
• 16. Tabaei-Aghdaei, S.R., Harrison, P. and Pearce, R.S. Expression of dehydration-stress-related genes in the crowns of wheatgrass species [Lophopyrum elongatum (Host) A. Love and Agropyron desertorum (Fisch. ex Link.) Schult.] having contrasting acclimation to salt, cold and drought. Plant Cell Environ. 23 (2000) 561-571.
• 17. Zhu, B., Choi, D.W., Fenton, R. and Close, T.J. Expression of the barley dehydrin multigene family and the development of freezing tolerance. Mol. Gen. Genet. 264 (2000) 145-153.
• 18. Rorat, T. Changes in gene expression in wild potato (Solanum sogarandinum) during cold acclimation. Acta Physiol. Plant. 23 (2001) 117-126.
• 19. Rorat, T., Grygorowicz, W.J., Irzykowski, W. and Rey, P. Expression of KS-type dehydrins is primarily regulated by factors related to organ type and leaf developmental stage during vegetative growth. Planta 218 (2004) 878885.
• 20. Korobczak, A., Aksamit, A., Łukaszewicz, M., Lorenc, K., Rorat, T. and Szopa, J. The potato glucosyltransferase gene promoter is environmentally regulated. Plant Sci. 168 (2005) 339-348.
• 21. Szwacka, M. Morawski, M. and Burza, W. Agrobacterium tumefaciens-mediated cucumber transformation with thaumatin II cDNA. Genet. Pol. 37A (1996) 126-129.
• 22. Yin, Z., Bartoszewski, G., Szwacka, M. and Malepszy, S. Cucumber transformation methods - the review. Biotechnologia (2004), in press.
• 23. Murashige, T. and Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15 (1962) 473-497.
• 24. Dellaporta, S.L., Wood, J. and Hicks, J.B. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1 (1983) 19-21.
• 25. Rorat, T., Sadowski, J., Irzykowski, W., Ziegler, P. and Daussant, J. Differential expression of two ß-amylase genes of rye during seed development. Physiol. Plant. 94 (1995) 19-24.
• 26. Rorat, T. and Irzykowski, W. Changes in mRNA population during cold acclimation in two potato lines of Solanum sogarandinum differing by their cold hardiness. Acta Physiol. Plant. 18 (1996) 25-32.
• 27. Monroy, A.F., Castonguay, Y., Laberge, S., Sarhan, F., Vezina, L.P. and Dhindsa, R.S. A new cold-induced alfalfa gene is associated with enhanced hardening at subzero temperature. Plant Physiol. 102 (1993) 873-879.
• 28. Pearce, R.S., Dunn, M.A., Rixon, J.E., Harrison, P. and Hughes, M.A. Expression of cold-inducible genes and frost hardiness in the crown meristem of young barley (Hordeum vulgare L. cv. Igri) plants grown in different environments. Plant Cell Environ. 12 (1996) 275-290.
• 29. Houde, M., Dhindsa, R.S. and Sarhan, F. A molecular marker to select for freezing tolerance in Gramineae. Mol. Gen. Genet. 234 (1992) 43-48.
• 30. Robertson, A.J., Weninger, A., Wilen, R.W., Fu, P. and Gusta, L.V. Comparison of dehydrin gene expression and freezing tolerance in Bromus inermis and Secale cereale grown in controlled environments, hydroponics and the field. Plant Physiol. 106 (1994) 1213-1216.
• 31. Nishibayashi, S., Hayakawa, T., Nakajima, T., Suzuki, M. and Kaneko, H. CMV protection in transgenic cucumber plants with an introduced CMV-O cp gene. Theor. Appl. Genet. 93 (1996) 672-678.