Sectrophotometric assay of renal ouabain-resistant Naplus-ATPase and its regulation by leptin and dietary-induced obesity

• Autorzy: Beltowski J. , Jamroz-Wisniewska A. , Nazar J. , Wojcicka G.
• Języki publikacji: EN

Abstrakty

EN

Apart from Na,K-ATPase, a second sodium pump, Na-stimulated, ^-independ­ent ATPase (Na -ATPase) is expressed in proximal convoluted tubule of the mamma­lian kidney. The aim of this study was to develop a method of Na -ATPase assay based on the method previously used by us to measure Na ,K -ATPase activity (Acta Biochim Polon.; 2002, 49: 515-27). The ATPase activity was assayed as the amount of inorganic phosphate liberated from ATP by isolated microsomal fraction. Na -ATPase activity was calculated as the difference between the activities mea­sured in the presence and in the absence of 50 mM NaCl. Na -ATPase activity was detected in the renal cortex (3.5 ± 0.2 ^mol phosphate/h per mg protein), but not in the renal medulla. Na -ATPase was not inhibited by ouabain or an H ,K -ATPase in­hibitor, Sch 28080, but was almost completely blocked by 2 mM furosemide. Leptin administered intraperitoneally (1 mg/kg) decreased the Na ,K -ATPase activity in the renal medulla at 0.5 and 1 h by 22.1% and 27.1%, respectively, but had no effect on Na -ATPase in the renal cortex. Chronic hyperleptinemia induced by repeated subcutaneous leptin injections (0.25 mg/kg twice daily for 7 days) increased cortical Na,K-ATPase, medullary Na,K-ATPase and cortical Na+-ATPase by 32.4%, 84.2% and 62.9%, respectively. In rats with dietary-induced obesity, the Na ,K - ATPase activity was higher in the renal cortex and medulla by 19.7% and 34.3%, re­spectively, but Na -ATPase was not different from control. These data indicate that both renal Na -dependent ATPases are separately regulated and that up-regulation of Na -ATPase may contribute to Na retention and arterial hypertension induced by chronic hyperleptinemia.

Słowa kluczowe

EN

leptin; sodium pump; dietary-induced obesity; mammal; potassium; ATPase; renal tubular cell; kidney; obesity

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 4
• Opis fizyczny: p.1003-1014,fig.,ref.

Twórcy

autor

Beltowski J.

• Medical University of Lublin, Jaczewskiego 8, 20-090 Lublin, Poland

autor

Jamroz-Wisniewska A.

autor

Nazar J.

autor

Wojcicka G.

Bibliografia

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