Evaluation of Acanthamoeba isolates from environmental sources in Tenerife, Canary Islands, Spain

• Autorzy: Lorenzo-Morales J. , Monteverde-Miranda C.A. , Jimenez C. , Tejedor M.L. , Valladares B. , Ortega-Rivas A.
• Języki publikacji: EN

Abstrakty

EN

Tests for potentially pathogenic amoebae were carried out in order to determine the presence of free-living amoebae of genus Acanthamoeba in soil and beach sand sources related to human environments in Tenerife, Canary Islands, Spain. Acanthamoeba identification was based on the morphology of cyst and trophozoite forms and PCR amplification with a genus specific primer pair. The pathogenical potential of Acanthamoeba isolates was characterized by temperature and osmotolerance assays and PCR reactions with two primer pairs related to Acanthamoeba pathogenesis. The results demonstrate the presence of potential pathogenic strain in both sources. Thus, some of the amoebae found in these habitats could act as opportunistic pathogens and may present a risk to human health.

Słowa kluczowe

EN

Canary Islands; Tenerife Island; Spain; environmental source; Acanthamoeba; amoeba; pathogenic amoeba; pathogenesis; human health

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2005
• Tom: 12
• Numer: 2
• Opis fizyczny: p.233-236,fig.,ref.

Twórcy

autor

Lorenzo-Morales J.

• University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Spain

autor

Monteverde-Miranda C.A.

autor

Jimenez C.

autor

Tejedor M.L.

autor

Valladares B.

autor

Ortega-Rivas A.

Bibliografia

• 1. Alsam S, Kim KS, Stins M, Rivas AO, Sissons J, Khan NA: Acanthamoeba interactions with human brain microvascular endothelial cells. Microb Pathog 2003, 35, 235-241.
• 2. Alves JM., Gusmao CX, Teixeira MM, Freitas D, Foronda A, Affonso HT: Random Amplified Polymorphic DNA profiles as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba. Braz J Med Biol Res 2000, 33, 19-26.
• 3. Armstrong M: The pathogenesis of human Acanthamoeba infection. Infect Dis Rev 2000, 2, 65-73.
• 4. Booton GC, Kelly DJ, Chu YW, Seal DV, Houang E, Lam DS, Byers TJ, Fuerst PA: 18S ribosomal DNA typing and tracking of Acanthamoeba species isolates from corneal scrape specimens, contact lenses, lens cases, and home water supplies of Acanthamoeba keratitis patients in Hong Kong. J Clin Microbiol 2002, 40, 1621-1625.
• 5. Booton GC, Rogerson A, Bonilla TD, Seal DV, Kelly DJ, Beattie TK, Tomlinson A, Lares-Villa F, Fuerst PA, Byers TJ: Molecular and Physiological Evaluation of Subtropical Environmental Isolates of Acanthamoeba spp., Causal agent of Acanthamoeba keratitis. J Eukaryot Microbiol 2004, 51, 192-200.
• 6. Chappel CL, Wright JA, Coletta M, Newsome AL: Standardized method of measuring Acanthamoeba antibodies in sera from healthy human subjects. Clin Diagnos Lab Immunol 2001, 8, 724-730.
• 7. Hong YC, Kong HH, Ock MS, Kim IN, Chung D I: Isolation and characterization of a cDNA encoding a subtilisin-like serin proteinase (AhSUB) from Acanthamoeba healyi. Mol Biol Parasitol 2000, 111, 441-446.
• 8. Howe DK, Vodkin MH, Novak RJ, Visvesvara G, McLaughlin GL: Identification of two genetic markers that distinguish pathogenic and non-pathogenic strains of Acanthamoeba spp. Parasitol Res 1997, 83, 345-348.
• 9. Khan NA, Jarroll EL, Paget TA: Acanthamoeba can be differentiated by the polymerase chain reaction and simple plating assays. Curr Microbiol 2001, 43, 204-208.
• 10. Khan NA: Pathogenesis of Acanthamoeba infections. Microb Pathog 2003, 34, 277-285.
• 11. Kong HH, Shin JY, Yu HS, Kim J, Hahn TW, Hahn YH, Chung DI: Mitochondrial DNA Restriction Fragment Length Polymorphism (RFLP) and 18S Small-Subunit Ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in Southwestern Korea. J Clin Microbiol 2002, 40, 1199-1206.
• 12. Levine S, Goldstein AE, Dahdouh M, Blank P, Hoffman C, Gropper CA: Cutaneous Acanthamoeba in a patient with AIDS: a case study with a review of new therapy. Cutis 2001, 67, 377-380.
• 13. Lorenzo-Morales J, Ortega-Rivas A, Foronda P, Martinez E, Valladares B: Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources. Parasitol Res 2005, 95, 273-277.
• 14. Ma P, Visvesvara G, Martinez A, Theodore FH, Dagget P, Sawyer T: Naegleria and Acanthamoeba infections: Review. Rev Infect Dis 1990, 12, 490-513.
• 15. Marciano-Cabral F and Cabral G: Acanthamoeba sp. as agents of disease in humans Clin Microbiol Rev 2003, 16, 273-307.
• 16. Marciano-Cabral F, Puffenbauer R, Cabral GA: The increasing importance of Acanthamoeba infections. J Euk Microbiol 2000, 47, 29-36.
• 17. Martínez A and Visvesvara G: Free living, amphizoic and opportunistic amoebas. Brain Pathol 1997, 7, 593-598.
• 18. Mergeryan H: The prevalence of Acanthamoeba in the human environment. Rev Infect Dis 1991, 5, 390-391.
• 19. Niederkorn JY, Alizadeh H, Leher H, McCulley JP: The pathogenesis of Acanthamoeba keratitis. Microb Infect 1999, 1, 437-443.
• 20. Ortega-Rivas A: Aplicación de la técnica de RAPD para la identificación de especies del género Acanthamoeba. PhD Thesis, University of La Laguna, Tenerife, Spain 2003.
• 21. Ortega-Rivas A, Lorenzo-Morales J, Alonso V, Abreu N, Foronda P, Del Castillo A Valladares B: Random Amplified Polymorphic DNA as a tool for the identification of Acanthamoeba divionensis. Curr Microbiol 2003, 47, 84-86.
• 22. Ortega-Rivas A, Lorenzo-Morales J, Villa M, Martinez E, Valladares B, Del Castillo A: Design and Evaluation of a specific primer pair for the identification of Acanthamoeba polyphaga. Curr Microbiol 2004, 48, 360-363.
• 23. Parija SC, Prakash MR, Rao VA, Vellaniparambil RJ: Acanthamoeba keratitis in Pondicherry. J Comm Dis 2001, 33, 126-129.
• 24. Pussard M, Pons R: Morphologie de la paroi kystique et taxonomie du genre Acanthamoeba. Protistologica 1977, 13, 557-598.
• 25. Radford C, Lehman O, Dart JKG: Acanthamoeba keratitis: multicentre survey in England 1992-6. National Acanthamoeba Keratitis Study Group. Br J Ophthalmol 1998, 82, 1387-1392.
• 26. Rivera MA, Padhya TA: Acanthamoeba: a rare primary cause of rhinosinusitis. Laryngoscope 2002, 112, 1201-1203.
• 27. Rodríguez R, Méndez O, Molina O, Luzardo G, Martínez A, Visvesvara G, Cardozo J: Infección del sistema nervioso central por amebas de vida libre: comunicación de tres nuevos casos venezolanos. Rev Neurol 1998, 26, 1005-1008.
• 28. Sambrook J, Fristsch EF, Maniatis T: Molecular cloning. A laboratory manual. 2nd ed. Cold Spring Harbord Laboratory Press 1989.
• 29. Seijo-Martínez M, González-Medeiro G, Santiago P, Rodríguez De Lope A, Diz J, Conde C, Visvesvara G: Granulomatous amebic encephalitis in a patient with AIDS: isolation of Acanthamoeba sp. Group II from brain tissue and successful treatment with sulfadiazine and fluconazole. J Clin Microbiol 2000, 38, 3892-3895.
• 30. Sell JJ, Rupp FW, Orrison WW Jr: Granulomatous amoebic encephalitis caused by Acanthamoeba. Neuroradiology 1997, 39, 434-436.
• 31. Shasuzzaman SM, Hashiguchi Y: Thoracic amoebiasis. Clin Chest Med 2002, 23, 479-492.
• 32. Steinberg JP, Galindo RL, Kraus ES, Ghanem KG: Disseminated acanthamoebiasis in a renal transplant recipient with osteomyelitis and cutaneous lesions: case report and literature review. Clin Infect Dis 2002, 35, 43-49.
• 33. Stothard DR, Schroeder-Diedrich JM, Awwad MH, Gast RJ, Ledee DR, Rodríguez-Zaragoza S, Dean C, Fuerst PA, Byers TJ: The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J Euk Microbiol 1998, 45, 45-54.
• 34. Szenasi Z, Endo T, Yagita K, Nagy E: Isolation, identification and increasing importance of free-living amoebae, causing human disease. J Med Microbiol 1998, 47, 45-54.
• 35. Vodkin MH, Howe DK, Visvesvara G, McLaughlin GL: Identification of Acanthamoeba at the generic and specific levels using the Polymerase Chain Reaction. J Protozool 1992, 39, 378-385