Transmission of horseradish peroxidase across the tegument of some hymenolepidid species [Cyclophyllidea]

• Autorzy: Walski M , Cielecka D. , Chomicz L. , Grytner-Ziecina B.
• Języki publikacji: EN

Abstrakty

EN

Strobilae of three hymenolepidid tapeworm species: Diorchis elisae, Fimbriaria fasciolaris and Sobolevicanthus gracilis, obtained from experimentally infected domestic ducks, were incubated for 5,10 and 15 min at a temperature of 38°C, with horseradish peroxidase applied as a tracer by which to research macromolecular transport across the tegument. Through the use of an electron microscope, it was indicated that the marked protein was bound at the surface of the microtriches with the co-participation of glycoproteins of the plasma membrane. It was also shown that the protein molecules gathered at the base of the microtriches were transported in vesicular form inside the syncytial layer and perikaryon part of the tegumental cytoplasm. Molecules of horseradish peroxidase liberated from the vesicles were observed in the excretory system of the tapeworms. In spite of interspecific differences in the structure of the tegument, the tracer protein transmission observed in this research was found to proceed in a similar manner in all the hymenolepidids studied.

Słowa kluczowe

EN

parasite; horseradish; Hymenolepis diminuta; Sobolevicanthus gracilis; hymenolepidid species; Fimbriaria fasciolaris; Diorchis elisae; tegument; Cyclophyllidea

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1995
• Tom: 40
• Numer: 4
• Opis fizyczny: p.206-210,fig.

Twórcy

autor

Walski M

• Polish Academy of Sciences, Dworkowa 3, 00-784 Warsaw, Poland

autor

Cielecka D.

autor

Chomicz L.

autor

Grytner-Ziecina B.

Bibliografia

• Charles G. H., Orr T. S. C. 1971. The ultrastructure of the developing pseudophyllid tegument (epidermis) with reference to the larval stages of Schistocephalus solidus and Ligula intestinalis. Second International Congress of Parasitology. Journal of Parasitology, Suppl., 57, 38-39.
• Cielecka D., Grytner-Zięcina В., Chomicz L. 1994. Studies on the surface ultrastructure of Sobolevicanthus gracilis (Zeder, 1803) (Cestoda, Hymenolepididae). Acta Parasitologica, 39, 131-137.
• Conway-Jones P. B., Rothman A. H. 1978. Hymenolepis microstoma: tegumentary disks. Experimental Parasitology, 44, 108-115.
• Graham R. C., Karnowsky M. J. 1966. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. Journal of Histochemistry and Cytochemistry, 14, 291-302.
• Grytner-Zięcina В., Cielecka D., Chomicz L. 1995. Transmission electron microscopy studies on the tegument of two species of genus Fimbriaria Froelich, 1802. Acta Parasitologica, 40, 88-93.
• Hopkins C. A., Law L. M., Threadgold L. T. 1978. Schistocephalus solidus: pinocytosis by the plerocercoid tegument. Experimental Parasitology, 44, 161-172.
• Kjellen L., Lindahl U. 1991. Proteoglycans: structures and interactions. Annual Review of Biochemistry, 60, 443-475.
• Lumsden R. D. 1966. Cytological studies on the absorptive surfaces of cestodes. II. The synthesis and intracellular transport of protein in the strobilar integument of Hymenolepis diminuta. Zeitschrift für Parasitenkunde, 28, 1-13.
• Lumsden R. D. 1975. Surface ultrastructure and cytochemistry of parasitic helminths. Experimental Parasitology, 37, 267-339.
• Lumsden R. D., Threadgold L. T., Oaks J., Arme C. 1970. On the permeability of cestodes to colloids: an evolution of the transmembranosis hypothesis. Parasitology, 60, 185-193.
• Machnicka В., Krawczuk S., Walski M. 1986. Influence of 0.02 M EDTA and 3 M KCl on surface of Hymenolepis diminuta and composition of isolated proteins. Folia Histochemica et Cytobiologica, 24, 65-70.
• Pappas P. W., Read С. P. 1975. Membrane transport in helminth parasites: A review. Experimental Parasitology, 37, 469-530.
• Pluta R., Lossinsky A. S., Wisniewski H. M., Mossakowski M. J. 1994. Early blood-brain barrier changes in the rat following transient complete cerebral ischaemia induced by cardiac arrest. Brain Research, 633, 41-52.
• Saunders M., Jalkanen M., O’Farrell S., Bernfield M. 1989. Molecular cloning of syndecan, an integral membrane proteoglycan. Journal of Cell Biology, 108, 1547-1556.
• Strużyńska L., Walski M., Gadamski R., Rafałowska U. 1995. Lead induced abnormalities in the blood - brain barrier permeability in experimental chronic toxicity. Acta Neurologica Scandinavica, in press.
• Threadgold L. T., Arme C. 1974. Hymenolepis diminuta: An electron microscope study of ion absorption. Experimental Parasitology, 35, 475-491.
• Threadgold L. T., Hopkins C. A. 1981. Schistocephalus solidus and Ligula intestinalis: pinocytosis by the tegument. Experimental Parasitology, 51, 444-456.
• Threadgold L. T., Dunn J. 1983. Taenia crassiceps: Regional variations in ultrastructure and evidence of endocytosis in the cysticercus tegument. Experimental Parasitology, 55, 121-131.
• Unger E., Pittersson I., Eriksson U. J., Lindahl U., Kjellen L. 1991. Decreased activity of the heparan sulfate-modifying enzyme glucosaminyl N-deacetylase in hepatocytes from streptozotocin-diabetic rats. Journal of Biological Chemistry, 266, 8671-8674.
• Walski M., Kłos J. 1977. Transmission of horseradish peroxidase across the small intestinal mucosa under tissue culture conditions. Acta Medica Polona, 18, 371-372.
• Walski M., Olszewska K. 1978. Horseradish peroxidase application to the protein transport investigations and alkaline phosphatase ultralocalization at the kidney barrier level. Folia Histochemica et Cytochemica, 16, 25-30.
• Walski M., Misiura M. 1981. Carbohydrate groups revealed in Fimbriaria fasciolaris with cytochemical methods. Folia Histochemica et Cytochemica, 19, 90-91.
• Walski M., Hassa Z., Cielecka D. 1986. An attempt to exhibit the point of absorption and the passage for transporting proteine substances in tapeworms. Folia Histochemica et Cytobiologica, 24, 326-327.
• Wilson J. M., King B. F. 1985. Transport of horseradish peroxidase across monkey trophoblastic epithelium in coated and uncoated vesicles. Anatomical Record, 211, 174-183.
• Yanagishita M., Hascall V. C. 1992. Cell surface heparan sulfate proteoglycans. Journal of Biological Chemistry, 267, 9451-9454.