Callus induction and gametophyte regeneration in moss cultures

• Autorzy: Sokal I. , Kuta E. , Przywara L.
• Języki publikacji: EN

Abstrakty

EN

Callus induction was achieved from the protonema, whole gametophyte and gametophyte shoot cells in Atrichum undulatum, Bryum caespiticium and Polytrichum commune on modified MS medium containing 4% (w/v) glucose as the carbon source, MS + 4% (w/v) glucose + 1 mg/l 2,4-D + 1 mg/l kinetin, and on MS mineral salts with 10% (v/v) coconut milk. All cultures except cultures containing coconut milk were maintained in the light. Regeneration of gametophytes in A. undulatum, B. caespiticium, Plagiomnium affine and P. commune from protonemata, protonemata with abnormal buds, whole gametophytes, shoots of gametophytes and callus was observed on MS mineral salts, Knudson mineral medium, regular MS and Knudson media. Restored gametophytes were also obtained on MS mineral medium containing 10% (v/v) coconut milk from calluses or callus-type protonema. All cultures were maintained in the light.

Słowa kluczowe

EN

callus; mineral medium; cytological analysis; regeneration; gametophyte; in vitro; bryophyte; Bryum caespiticium; plant regeneration; sterilization; filament; Polytrichum commune; moss; Atrichum undulatum; protonema

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1997
• Tom: 39
• Opis fizyczny: p.35-42,fig.

Twórcy

autor

Sokal I.

• Jagiellonian University, ul.Grodzka 52, 31-044 Krakow, Poland

autor

Kuta E.

autor

Przywara L.

Bibliografia

• Allsopp, A. 1957. Controlled differentiation in cultures of two liverworts. Nature 179: 681-682.
• Bopp, M. 1982. How can external hormones regulate the morphogenesis of mosses? Journal of the Hattori Botanical Laboratory 53: 159-169.
• Bopp, M. 1984. Developmental physiology of Bryophytes. In: R. M. Schuster [ed.], New Manual of Bryology 2, 276-324. Hattori Botanical Laboratory, Nichinan.
• Bopp, M., B. Knoop. 1984. Culture methods for Bryophytes. In: I. K. Vasil. [ed.], Cell culture and somatic cell genetics of plants 1, 96-105. Academic Press, London.
• Gemmell, A. R. 1955. Regeneration from the leaf of Atrichum undulatum (Hedw.) P. Beauv. British Bryological Socciety 2: 203-213.
• Lal, M. 1961. In vitro production of apogamous sporogonia in Physcomitrium coorgense Broth. Phytomorphology 11: 263-269.
• Lal, M. 1984. The culture of bryophytes including apogamy, apospory, parthenogenesis and protoplasts. In: A. F. Dyer, and J. G. Duckett [eds.], The experimental biology of bryophytes, 97-115. Academic Press, London.
• Matsui, K., Y. Kaji, T. Kajiwara, and A. Hatanaka. 1996. Developmental changes of lipoxygenase and fatty acid hydroperoxidaze lyase activities in cultured cells of Marchantía polymorpha. Phytochemistry 41: 177-182.
• Matsuo, A., K. Ono, K. Hamasaki, and H. Nozaki. 1996. Phaeophytins from a cell suspension culture of the liverwort Plagiochila ovalifolia. Phytochemistry 42: 427-430.
• Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarían 15: 473-497.
• Nabeta, K., T. Oohata, T. Sato, S. Ohkubo, and K. Katoh. 1996. Spiro-lactone diterpenes from in vitro cultures of the liverwort, Heteroscyphus planus. Phytochemistry 41: 581-587.
• Nehira, K. 1964. Culture media for bryophytes. Bulletin of the Biological Society of Hiroshima University 31: 15-21.
• Nemira, K. 1984. Spore germination, protonema development and sporeling development. In: R. M. Schuster [ed.], New Manual of Bryology 2, 343-378. Hattori Botanical Laboratory, Nichinan.
• Ohta, Y., and Y. Hirose. 1982. Induction and characteristics of cultured cells from some liverworts of Jungermanniales. Journal of the Hattori Botanical Laboratory 53: 239-244.
• Ono, K. 1973. Callus formation in liverwort, Marchantía polymorpha. Japanese Journal of Genetics 48: 69-70.
• Ono, K. 1976. Cytological observations on the calluses and the restored thalluses in Marchantía polymorpha. Japanese Journal of Genetics 51: 11-18.
• Oszkinis, K. 1991. Storczyki. Państwowe Wydawnictwo Rolnicze i Leśne, Warszawa.
• Rashid, A., R. N. Chopra. 1969. The apogamous sporophytes of Funaría hygrometrica and their cultural behaviour. Phytomorphology 19: 170-178.
• Schelpe, E. A. C. L. E. 1953. Techniques for the experimental culture of bryophytes. Transactions of the British Bryological Society 2: 216-217.
• Selkirk, P.M. 1980. Effect of an exogenous auxin and cytokinin on Riccia. The Bryologist 83: 67-71.
• Szweykowska, A. 1962. The efects of kinetin and IAA on shoot development in Funaría hygrometrica and Ceratodon purpureus. Acta Societatis Botanicorum Poloniae 31: 553-557.
• Szweykowska, A., A. Handszu. 1965. The effect of kinetin on gametophyte development and protein synthesis in the moss Ceratodon purpureus. Acta Societatis Botanicorum Poloniae 34: 73-81.
• Szweykowska, A., I. Korcz, B. Jaśkiewicz-Mroczkowska, M. Metelska. 1972. The effect of various cytokinins and other factors on the protonema cell divisions and the induction of gametophores in Ceratodon purpureus. Acta Societatis Botanicorum Poloniae 41: 400-409.
• Szweykowska, A., T. Maćkowiak. 1962. On the development of gametophytes in Funaría hygrometrica and Ceratodon purpureus in liquid cultures. Acta Societatis Botanicorum Poloniae 31: 269-274.
• Szweykowska, A., W. Ratajczak, J. Schneider. 1968. Studies on the activity of kinetin in cultures of Funaria hygrometrica. Acta Societatis Botanicorum Poloniae 37: 145-155.
• Szweykowska, A., J. Schneider, U. Prusińska. 1969. Studies on the specificity and sensitivity of the bud - induction response to cytokinins in the protonema of Funaria hygrometrica. Acta Societatis Botanicorum Poloniae 38: 13-42.
• Tazaki, H., H. Soutome, K. Nabeta, H. Okuyama, and H. Becker. 1996. Pinguisone derivatives from an axenic culture of the liverwort Aneura pinguis. Phytochemistry 42: 465-468.
• Ward, M. 1954. Fertilization in Phlebodium auteum. J. Sm. Phytomorphology 4: 1-17.
• Ward, M. 1960. Some techniques in the culture of mosses. The Bryologist 63: 213-217.
• Ward, M. 1960a. Callus tissue from the mosses Polytrichum and Atrichum. Science 132: 1401-1402.