Antioxidant compounds in Salvia officinalis L. shoot and hairy root cultures in the nutrient sprinkle bioreactor

• Autorzy: Grzegorczyk I. , Wysokinska H.
• Języki publikacji: EN

Abstrakty

EN

The study focused on the production of compounds with antioxidant activity in hairy root and shoot cultures of Salvia officinalis grown in laboratory-scale sprinkle nutrient bioreactors. HPLC analysis showed that production of rosmarinic acid in transformed roots (34.65±1.07 mg l-1) was higher that in shoot culture (26.24±0.48 mg l-1). In the latter diterpenoids: carnosic acid (1.74±0.02 mg l-1) and carnosol (1.34±0.01 mg l-1) were also found. Biomass accumulation after a growth period in the bioreactor was also studied. An 18-fold increase in hairy root biomass was recorded after 40 days of culture. In sage shoot culture, biomass increased 43 times after 21 days of bioreactor run. The current operating conditions of the bioreactor were not suitable for the propagation of Salvia officinalis mainly due to the hyperhydricity problem of leaves and stems.

Słowa kluczowe

EN

antioxidant compound; Salvia officinalis; shoot; hairy root; root culture; nutrient sprinkle bioreactor; bioreactor; leaf; stem

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2010
• Tom: 79
• Numer: 1
• Opis fizyczny: p.7-10,fig.,ref.

Twórcy

autor

Grzegorczyk I.

• Medical University in Lodz, Muszynskiego 1, 90-151 Lodz, Poland

autor

Wysokinska H.

Bibliografia

• CHATTOPADHYAY S., SRIVASTAVA A.K., BHOJWANI S.S., BISARIA V.S. 2002. Production of Podophyllotoxin by plant cell cultures of Podophyllum hexandrum in bioreactor. J. Biosci. Bioeng. 93: 215-220.
• CHMIEL A., LEDAKOWICZ S, PAWLOWSKA B. 2001. The mist bioreactor for cultures of transformed roots. In: 28th Con- ference SSCHE, Proceedings on CD ROM, May 21-25, Ta- transke Matliare (SK).
• CUVELIER M.E., BERSET C., RICHARD H. 1994. Antioxidant constituents in sage (S. officinalis). J. Agric. Food Chem. 42: 665-669.
• GRZEGORCZYK I., BILICHOWSKI I., MIKICIUK-OLASIK E., WYSOKINSKA H. 2005. In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds. Acta Soc. Bot. Pol. 74: 17-21.
• GRZEGORCZYK I., KROLICKA A., WYSOKINSKA H. 2006. Establishment of Salvia officinalis L. hairy root cultures for the production of rosmarinic acid. Z. Naturforsch. 61c: 351-356.
• GRZEGORCZYK I., MATKOWSKI A.,WYSOKINSKA H. 2007. Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chem. 104: 536-541. GRZEGORCZYK I., WYSOKINSKA H. 2004. Mikrorozmnazanie Salvia officinalis L. Z wierzchołków pt(d6w. Biotechnologia. 65: 212-218.
• GRZEGORCZYK I., WYSOKINSKA H. 2008. Liquid shoot cultures of Salvia officinalis L. for micropopagation and production of antioxidant compounds; effect of triacontanol. Acta Soc. Bot. Pol. 77: 99-104.
• TEN HOOPEN H.J.G., VAN GULIK W.M., SCHLATMANN J.E., MORENO P.R.H., VINKE J.L., HEIJNEN J.K.J., VER- POORTE R. 1994. Ajmalicine production by cell cultures of Catharanthus roseus: from shake flask to bioreactor. Plant Cell Tiss. Organ Cult. 38: 85-91.
• LLOYD G.B., MCCOWN B.H. 1980. Commercially-feasible micropropagation of mountain laurel - Kalmia latifolia by use of shoottip culture. Proc. Int. Plant Prop. Soc. 30: 421-427.
• MIURA K., KIKUZAKI H., NAKATANI N. 2002. Antioxidant activity of chemical components from sage and thyme measured by the oil stability index method. J. Agric. Food Chem. 50: 1845-1851.
• MURASHIGE T., SKOOG F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
• PIf\TCZAK E., WIELANEK M., WYSOKINSKA H. 2005. Li- quid culture system for shoot multiplication and secoiridoid production in micropropagated plants of Centaurium erythraea Rafn. Plant Sci. 168: 431-437.
• SALES E., NEBAUER S.G., ARRILAGA I., SEGURA J. 2002. Plant hormones and Agrobacterium tumefaciens strain 82139 induce efficient plant regeneration in the cardenolide - producing plant Digitalis minor. J. Plant Physiol. 159: 9-16.
• SCHWARZ K. TERNES W. 1992. Antioxidative constituents of Rosmarinus officinalis and Salvia officinalis II: Isolation of carnosic acid and formation of other phenolic diterpenes. Z. Lebens. Unters. Forsch. 195: 99-103.
• SCRAGG A.H. 1995. The problems associated with high biomass level in plant cell suspensions. Plant Cell Tiss. Organ Cult. 43: 163-170.
• SCRAGG A.H., MORRIS P., ALLAN E.J., BOND P., FOWLER M.W. 1987. Effect of scale-up on serpentine formation by Ca- tharanthus roseus suspension culture. Enz. Microbial Technol. 9: 619-624.