Novel galactonic acid-binding hexameric lectin from Hibiscus mutabilis seeds with antiproliferative and potent HIV-1 reverse transcriptase inhibitory activities

• Autorzy: Lam S. K. , Ng T.B.
• Języki publikacji: EN

Abstrakty

EN

 A hexameric 150-kDa lectin was isolated from dried Hibiscus mutabilis seeds using a chromatographic protocol that involved ion exchange chromatography on SP-Sepharose, and gel filtration on Superdex 75 and Superdex 200. The lectin was not adsorbed on SP-Sepharose and was eluted from the Superdex 75 column in the void volume. It was eluted in the first peak from Superdex 200. It was strongly adsorbed on DEAE-cellulose and Q-Sepharose and could not be easily desorbed. The hemagglutinating activity of the lectin, which was stable at pH 4-7 and up to 50oC, could be inhibited by 25 mM galactonic acid. This is the first report of a galactonic acid-binding lectin. It potently inhibited HIV-1 reverse transcriptase with an IC50 of 0.2 µM. It exhibited weak antiproliferative activity towards both hepatoma HepG2 cells (40% inhibition) and breast cancer MCF-7 cells (50% inhibition) at 100 µM concentration of the lectin. It did not inhibit mycelial growth of a number of fungi tested.

Słowa kluczowe

EN

hexameric lectin; lectin; carbohydrate-binding protein; galactonic acid; seed; Hibiscus mutabilis; seed extract; lectin isolation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 4
• Opis fizyczny: p.649-654,fig.,ref.

Twórcy

autor

Lam S. K.

• The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

autor

Ng T.B.

Bibliografia

• Banerjee R, Mande SC, Ganesh V, Das K, Dhanaraj V, Mahanta SK, Suguna K, Surolia A, Vijayan M (1994) Crystal structure of peanut lectin, a protein with an unusual quaternary structure. Proc Natl Acad Sci USA 91: 227-231. 
• Böttcher M, Grosse F (1997) HIV-1 protease inhibits its homologous reverse transcriptase by protein-protein interaction. Nucleic Acids Res 25: 1709-1714. 
• Datta PK, Figueroa MO, Lajolo FM (1991) Purification and characterization of two major lectins from Araucaria brasiliensis syn. Araucaria angustifolia Seeds (Pinhão). Plant Physiol 97: 856-862.  
• De Hoff PL, Brill LM, Hirsch AM (2009) Plant lectins: the ties that bind in root symbiosis and plant defense. Mol Genet Genomics 282: 1-15. 
• Devi SK, Devi LI, Singh LR (2009) Purification and characterization of a new dimeric mannose/glucose-binding isolectin from Vicia tetrasperma (L.) Schreber. Prep Biochem Biotechnol 39: 57-71. 
• Devlin TM (1997) Textbook of biochemistry: with clinical correlations. 4th edn, p 48. Wiley-Liss, inc.
• Dresch RR, Zanetti GD, Lerner CB, Mothes B, Trindade VM, Henriques AT, Vozári-Hampe MM (2008) ACL-I, a lectin from the marine sponge Axinella corrugata: isolation, characterization and chemotactic activity Comp Biochem Physiol C Toxicol Pharmacol 148: 23-30.  
• Du XY, Magnenat E, Wells TN, Clemetson KJ (2002) Alboluxin, a snake C-type lectin from Trimeresurus albolabris venom is a potent platelet agonist acting via GPIb and GPVI. Thromb Haemost 87: 692-698.  
• Jin S, Cheng Y, Reid S, Li M, Wang B (2009) Carbohydrate recognition by boronolectins, small molecules, and lectins. Med Res Rev (Epub ahead of print). 
• Kholkute SD, Mudgal V, Udupa KN (1977) Studies on the antifertility potentiality of Hibiscus rosa sinensis. Parts of medicinal value; selection of species and seasonal variations. Planta Med 31: 35-39. 
• Lam YW, Ng TB (2002) Purification and characterization of a rhamnose-binding lectin with immunoenhancing activity from grass carp (Ctenopharyngodon idellus) ovaries. Protein Expr Purif 26: 378-385.  
• Lam SK, Han QF, Ng TB (2009) Isolation and characterization of a lectin with potentially exploitable activities from caper (Capparis spinosa) seeds. Biosci Rep 29: 293-299. 
• Lam SK, Ng TB (2009) A protein with antiproliferative, antifungal and HIV-1 reverse transcriptase inhibitory activities from caper (Capparis spinosa) seeds. Phytomedicine 16: 444-450. 
• Leung EH, Wong JH, Ng TB (2008) Concurrent purification of two defense proteins from French bean seeds: a defensin-like antifungal peptide and a hemagglutinin. J Pept Sci 14: 349-353. 
• Liu Q, Wang H, Ng TB (2004) Isolation and characterization of a novel lectin from the wild mushroom Xerocomus spadiceus. Peptides 25: 7-10. 
• Liu J, Xu X, Liu J, Balzarini J, Luo Y, Kong Y, Li J, Chen F, Van Damme E, Bao J (2007) A novel tetrameric lectin from Lycoris aurea with four mannose binding sites per monomer. Acta Biochim Polon 54: 159-166.  
• Mistry AC, Honda S, Hirose S (2001) Structure, properties and enhanced expression of galactose-binding C-type lectins in mucous cells of gills from freshwater Japanese eels (Anguilla japonica). Biochem J 360: 107-115.  
• Ngai PH, Ng TB (2007) A mannose-specific tetrameric lectin with mitogenic and antibacterial activities from the ovary of a teleost, the cobia (Rachycentron canadum). Appl Microbiol Biotechnol 74: 433-438.  
• Panchak LV, Antoniuk VO (2007) Purification of lectin from fruiting bodies of Lactarius rufus (Scop.: Fr.) Fr. and its carbohydrate specificity. Ukr Biokhim Zh 79: 123-128. 
• Sanchez JF, Lescar J, Chazalet V, Audfray A, Gagnon J, Alvarez R, Breton C, Imberty A, Mitchell EP (2006) Biochemical and structural analysis of Helix pomatia agglutinin. A hexameric lectin with a novel fold. J Biol Chem 281: 20171-20180.  
• Sharma A, Ng TB, Wong JH, Lin P (2009) Purification and characterization of a lectin from Phaseolus vulgaris cv. (Anasazi beans). J Biomed Biotechnol (Epud ahead of print). 
• Sultan NA, Kavitha M, Swamy NJ (2009) Purification and physicochemical characterization of two galactose-specific isolectins from the seeds of Trichosanthes cordata. IUBMB Life 61: 457-469. 
• Thakur A, Rana M, Lakhanpal TN, Ahmad A, Khan M (2007) Purification and characterization of lectin from fruiting body of Ganoderma lucidum: lectin from Ganoderma lucidum. Biochim Biophys Acta 1770: 1404-1412.  
• Trindade MB, Lopes JL, Soares-Costa A, Monteiro-Moreira AC, Moreira RA, Oliva ML, Beltramini LM (2006) Structural characterization of novel chitin-binding lectins from the genus Artocarpus and their antifungal activity. Biochim Biophys Acta 1764: 146-152. 
• Wang HX, Ng TB (2006) Concurrent isolation of a Kunitz-type trypsin inhibitor with antifungal activity and a novel lectin from Pseudostellaria heterophylla roots. Biochem Biophys Res Commun 342: 349-353. 
• Wang H, Gao J, Ng TB (2000) A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem Biophys Res Commun 275: 810-816. 
• Wang H, Ng TB, Liu Q (2002) Isolation of a new heterodimeric lectin with mitogenic activity from fruiting bodies of the mushroom Agrocybe cylindracea. Life Sci 70: 877-885. 
• Wong JH, Ng TB (2006) Isolation and characterization of a glucose/mannose-specific lectin with stimulatory effect on nitric oxide production by macrophages from the emperor banana. Int J Biochem Cell Biol 38: 234-243. 
• Wong JH, Wong CC, Ng TB (2006) Purification and characterization of a galactose-specific lectin with mitogenic activity from pinto beans. Biochim Biophys Acta 1760: 808-813.  
• Xu X, Wu C, Liu X, Luo Y, Li J, Zhao X, Damme EV, Bao J (2007) Purification and characterization of a mannose-binding lectin from the rhizomes of Aspidistra elatior Blume with antiproliferative activity. Acta Biochim Biophys Sin (Shanghai) 39: 507-519. 
• Yan Q, Jiang Z, Yang S, Deng W, Han L (2005) A novel homodimeric lectin from Astragalus mongholicus with antifungal activity. Arch Biochem Biophys 442: 72-81. 
• Yang X, Tang D, Xu D, Wang X, Pan G (2004) Characters of greening tree species in heavy metal pollution protection in Shanghai. Ying Yong Sheng Tai Xue Bao 15: 687-690. 
• Yeh PY, Ling WF, Takasaka R (1958) Pigments in the flower of fu-yong (Hibiscus mutabilis L.). Science 128: 312. 
• Zheng MS (1989) An experimental study of the anti-HSV-II action of 500 herbal drugs. J Tradit Chin Med 9: 113-116