Sensitivity of Tilia cordata seeds to dehydration and temperature of liquid nitrogen

• Autorzy: Chmielarz P
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to assess the susceptibility of small-leaved lime (Tilia cordata Mill.) seeds to drying and freezing in liquid nitrogen (-196°C). Seed samples were frozen in liquid nitrogen for 24 h at 11 different levels of seed moisture content (m.c.), ranging from 3.1% to 22.8% (fresh weight basis). All samples, including unfrozen control samples, were subjected to scarification with concentrated sulphuric acid (Tylkowski 1998) either before or after freezing. Seed pre-treatment before germination (at 3~15°C/16~8h) involved cold stratification at 3°C without substrate. Seed drying to 3.1% m.c. significantly reduced their germinability (to 63%), as compared to the high germinability (82-88%) of seeds with 5.2-20.9% m.c. Thus seeds of this species can be assigned to the ‘suborthodox' category. Such a high germinability (79-87%) was preserved after freezing in liquid nitrogen in samples dried to 9.0-17.4% m.c. if scarification was performed before freezing, and in samples dried to 9.1-16.2% m.c. if scarification was performed after freezing. The highest percentage of seedlings emerged after freezing in liquid nitrogen from seeds dried to 11.1-20.1% m.c. (emergence 65-75%) if scarification was performed before freezing, and from seeds dried to 7.3-17.8% m.c. (emergence 53%-71%) if scarification was performed after freezing.

Słowa kluczowe

EN

dehydration; drying; liquid nitrogen; temperature; Tilia cordata; forest habitat; sensitivity; scarification; cryopreservation; seed

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2002
• Tom: 47 Supplement
• Opis fizyczny: p.71-77,fig.,ref.

Twórcy

autor

Chmielarz P

• Polish Academy of Sciences, Parkowa 5, 62-035 Kornik, Poland

Bibliografia

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