Agrobacterium-mediated transformation of yellow lupin to generate callus tissue producing HBV surface antigen in a long-term culture

• Autorzy: Pniewski T , Kapusta J , Plucienniczak A
• Języki publikacji: EN

Abstrakty

EN

The idea of an oral vaccine administered as a portion of plant tissue requires a high level of antigen production. An improved protocol for the induction of transgenic yellow lupin calli or tumours, reaching 44% of transformation rate, is presented here. It has been developed by using the nptII marker gene and the uidA reporter gene as well as various Agrobacterium strains and plant explants. This method of seedling and hypocotyl transformation was applied to raise calli or tumours producing a small surface antigen of Hepatitis В Virus (S-HBsAg). Lupin tissue lines were long-term cultured on selection media maintaining the growth rate and high expression level of the native form of S-HBs, up to 6 µg per g of fresh tissue.

Słowa kluczowe

EN

yellow lupin; genetic transformation; transgenic lupin; gene expression; nptII marker gene; hepatitis B virus; antigen; uidA reporter gene; Lupinus luteus; Agrobacterium tumefaciens; lupin; surface antigen; plant tissue; antigen production; plant explant; protein content; callus tissue

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2006
• Tom: 47
• Numer: 4
• Opis fizyczny: p.309-318,fig.,ref.

Twórcy

autor

Pniewski T

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Kapusta J

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland
• Institute of Biotechnology and Antibiotics, Warszawa, Poland

autor

Plucienniczak A

• Institute of Biotechnology and Antibiotics, Warszawa, Poland

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