Synthesis, cloning and expression in Escherichia coli of the gene coding for the trypsin inhibitor from Cucurbita pepo

• Autorzy: Rempola B. , Wilusz T. , Markiewicz W. , Fikus M.
• Języki publikacji: EN

Abstrakty

EN

A chemically synthesized gene coding for the serine proteinase inhibitor CPTI II was cloned in E. coli and its expression was investigated in cytoplasmic and secretion systems. Under all conditions investigated the biologically active form of the inhibitor was found only in the latter system, although the yield was rather low.

Słowa kluczowe

EN

secretion system; trypsin inhibitor; cloning; gene coding; cytoplasmic system; Escherichia coli; expression; Cucurbita pepo; proteinase inhibitor; gene cloning

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 1
• Opis fizyczny: p.109-114,fig.

Twórcy

autor

Rempola B.

• Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Wilusz T.

autor

Markiewicz W.

autor

Fikus M.

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