Wybrane aspekty dojrzewania oocytow swini w warunkach fizjologicznych i pozaustrojowych

• Autorzy: Stankiewicz T , Blaszczyk B. , Udala J.

Warianty tytułu

EN

Selected aspects of pig oocytes maturation in vivo and in vitro

• Języki publikacji: PL

Abstrakty

EN

Oocytes attain developmental competence as a result of their maturation, which gives them nuclear and cytoplasm maturity. In physiological conditions the developmental competencies of oocytes are achieved in the environment of ovarian follicles before ovulation. In in vitro conditions, however, this occurs in a culture medium which, for porcine oocytes, is generally a TCM-199 and NCSU-23 medium supplemented in specific proportions of amino acids, proteins, hormones, growth factors and follicular fluid. The specific nature of porcine oocytes is that it takes them almost twice as long to obtain nuclear and cytoplasm maturity as in the case of other species of farm animals. Moreover, a common problem of in vitro maturation is the absence of normal cytoplasm maturity. Irregularities in translocation of mitochondria in the cytoplasm and transferring ions signals may also be observed. The absence of cytoplasm maturity of oocytes on the other hand reduces the possibility of male pronucleus (MPN) formation and development of zygotes to the blastocytes stage. Therefore, the latest studies concentrate on formulating methods of in vitro culture which enable normal development of porcine oocytes both in their nuclear as well as cytoplasm maturity.

Słowa kluczowe

PL

dojrzewanie in vivo; dojrzewanie in vitro; trzoda chlewna; oocyty; dojrzewanie oocytow; rozrod zwierzat

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 04A
• Opis fizyczny: s.400-403,bibliogr.

Twórcy

autor

Stankiewicz T

• Akademia Rolnicza w Szczecinie, ul.Doktora Judyma 6, 71-466 Szczecin

autor

Blaszczyk B.

autor

Udala J.

Bibliografia

• 1.Beckmann L. S., Day B. N.: Effects of media NaCl concentration and osmolarity on the culture of early-stage pig embryos and the viability of embryos cultured in a selectedsuperior medium. Theriogenology 1993, 39, 611-622.
• 2.Gandolfi F., Brevini T. A. L., Cillo F., Antonini S.: Cellular and molecular mechanisms regulating oocyte quality and the relevance for farm animal reproductive efficiency. Rev. sci. tech. Off. int. Epiz. 2005, 24, 413-423.
• 3.Grabiec A., Faundez R., Max A., Tischner M.: Dojrzewanie in vitro oocytów kota domowego. Medycyna Wet. 2007, 63, 318-321.
• 4.Griffin J., Emery B. R., Huang I., Peterson C. M., Carrell D. T.: Comparative analysis of follicle morphology and oocyte diameter in four mammalian species (mouse, hamster, pig, and human). J. Exp. Clin. Assist. Reprod. 2006, 3, 2, 5-9.
• 5.Hunter M. G.: Oocyte maturation and ovum quality in pigs. Rev. Reprod. 2000, 5, 122-130.
• 6.Ikeda K., Takahashi Y.: Comparison of maturational and developmental parameters of oocytes recovered from prepubertal and adult pigs. Reprod. Fertil. Dev. 2003, 15, 215-221.
• 7.Kątska L.: Comparison of two methods for recovery of ovarian oocytes from slaughter cattle. Anim. Reprod. Sci. 1984, 7, 461-463.
• 8.Kątska K., Kania G., Ryńska B., Gajda B., Smorąg Z.: Potomstwo po transplantacji zarodków kozich pochodzących z dojrzewających i zapładnianych in vitro oocytów. Medycyna Wet. 2002, 58, 462-463.
• 9.Kątska-Książkiewicz L., Lechniak-Cieślak D., Korwin-Kossakowska A., Alm H., Ryńska B., Warzych E., Sosnowski J., Sender G.: Genetical and biotechnological methods of utilization of female reproductive potential in mammals. Reprod. Biol. 2006, 6, Suppl 1, 21-36.
• 10.Kowalska B. D., Okólski A.: Wspomagany rozród niedojrzałych płciowo samic. Medycyna Wet. 2007, 63, 151-154.
• 11.Lucas X., Martinem E. A., Roca J., Vazquez J. M., Gil M. A., Pastor L. M., Alabart J. L.: Relationship between antral follicle size, oocyte diameters and nuclear maturation of immature oocytes in pigs. Theriogeneology 2002, 58, 871-885.
• 12.Lucas X., Martinem E. A., Roca J., Vazquez J. M., Gil M. L., Pastor L. M., Alabart J. L.: Influence of follicle size on the penetrability of immature pig oocytem for homologus in vitro penetration assay. Theriogenology 2003, 60, 659-667.
• 13.Lucidi P., Bernabò N., Turriani M., Barboni B., Mattioli M.: Cumulus cells steroidogenesis is influenced by the degree of oocyte maturation. Reprod. Biol. Endocrinol. 2003, 1, 45.
• 14.Marchal R., Caillaud M., Martoriati A., Gerard N., Mermillod P., Goudet G.: Effect of growth hormone (GH) on in vitro nuclear and cytoplasmic oocyte maturation, cumulus expansion, hyaluronan syntheses, and connexins 32 and 43 expression, and GH receptor messenger RNA expression in equine and porcine species. Biol. Reprod. 2003, 69, 1013-1022. 17
• 15.Marchal R., Vigneron C., Perreau C., Bali-Papp A., Mermillod P.: Effect of follicular size on meiotic and developmental competence of porcine oocytes. Theriogenology 2002, 57, 1523-1532.
• 16.Marques M. G., Nicacio A. C., de Oliveira V. P., Nascimento A. B., Caetano H. V., Mendes C. M., Mello M. R., Milazzotto M. P., Assumpcao M. E., Visintin J. A.: In vitro maturation of pig oocytes with different media, hormone and meiosis inhibitors. Anim. Reprod. Sci. 2007, 97, 375-381.
• 17.Morbeck D. E., Esbenshade K. L., Flowers W. L., Britt J. H.: Kinetics of follicle growth in the prepubertal gilt. Biol. Reprod. 1992, 47, 485-491.
• 18.Niwa K.: Effectiveness of in vitro maturation and in vitro fertilization techniques in pig. J. Reprod. Fertil. Suppl. 1993, 48, 49-59.
• 19.Obarzanek-Fojt M., Sosnowski J.: Możliwości wykorzystania jałówek jako dawczyń oocytów. Medycyna Wet. 2005, 61, 133-135.
• 20.Park C. K., Lee J. H., Cheng H. T., Yang B. K., Kim C. I.: Effect of superoxide dismutase (SOD) on pronucleus formation of porcine oocytem fertilized in vitro. Theriogenology 1997, 48, 1137-1146.
• 21.Peters J. K., Milliken G., Davis D. L.: Development of porcine oocytes in vitro: Effect culture medium and donor age. J. Anim. Sci. 2001, 79, 1578-1583.
• 22.Petters R. M., Wells K. D.: Culture of pig embryos. J. Reprod. Fert. Suppl. 1993, 48, 61-73.
• 23.Rath D., Niemann H., Tao T.: In vitro maturation of porcine oocytes in follicular fluid with subsequent effects on fertilization and embryo yield in vitro. Theriogenology 1995, 44, 529-538.
• 24.Rátky J., Torner H., Egerszegi I., Schneider F., Sarlos P., Manabe N., Brüssow K. B.: Ovarian activity and oocyte development during follicular development in pigs at different reproductive phases estimated by the repeated endoscopic method. J. Reprod. Dev. 2005, 51, 109-115.
• 25.Sherrer E. S., Rathbun T. J., Davis D. L.: Fertilization and blastocyst development in oocytes obtained from prepubertal and adult pigs. J. Anim. Sci. 2004, 82, 102-108.
• 26.Sun Q. Y., Nagai T.: Molecular mechanisms underlying pig oocyte maturation and fertilization. J. Reprod. Dev. 2003, 49, 347-359.
• 27.Sun Q. Y., Wu G. M., Lai L., Park K. W., Cabot R., Cheong H. T., Day B. N., Prather R. S., Schatten H.: Translocation of active mitochondria during pig oocyte maturation, fertilization and early embryo development in vitro. Reproduction 2001, 122, 155-163.
• 28.Suzuki H., Takashima Y., Toyokawa K.: Cytoskeleton organization of porcine oocytes aged activated electrically or by sperm. J. Reprod. Dev. 2002, 48, 293-301.
• 29.Yamauchi N., Nagai T.: Male pronuclear formation in denuded porcine oocytes after in vitro maturation in the presence of cysteamine. Biol. Reprod. 1999, 61, 828-833.
• 30.Yoshida M., Ishizaki Y., Kawagishi H., Bamba K., Kojima Y.: Effects of pig follicular fluid on maturation of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J. Reprod. Fertil. 1992, 95, 481-488.