Yeast as a biosensor for antioxidants: simple growth tests employing a Saccharomyces cerevisiae mutant defective in superoxide dismutase

• Autorzy: Zyracka E. , Zadrag R. , Koziol S. , Krzepilko A. , Bartosz G. , Bilinski T.
• Języki publikacji: EN

Abstrakty

EN

Mutants of Saccharomyces cerevisiae devoid of Cu,Zn-superoxide dismutase are hypersensitive to a range of oxidants, hyperbaric oxygen and hyperosmotic media, show lysine and methionine auxotrophy when grown under the atmosphere of air and have a shortened replicative life span when compared to the wild-type strain. Ascorbate and other antioxidants can ameliorate these defects, which may be a basis of simple tests sensing the presence of antioxidants. In particular, tests of growth on solid medium (colony formation) in the absence of methionine and/or lysine, or in the presence of 0.8 M NaCl can be useful for detection and semiquantitative estimation of compounds of antioxidant properties. Hypoxic atmosphere was found to increase the sensitivity of detection of antioxidants. The test of abolishment of lysine auxotrophy showed a concentration dependence of the antioxidant effects of cysteine and N-acetylcysteine which, however, lost their protective action at high concentration, in contrast to glutathione which was effective also at higher concentrations.

Słowa kluczowe

EN

yeast; superoxide dismutase; biosensor; ascorbate; antioxidant; mutant; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2005
• Tom: 52
• Numer: 3
• Opis fizyczny: p.679-684,fig.,ref.

Twórcy

autor

Zyracka E.

• University of Rzeszow, Rzeszow, Poland

autor

Zadrag R.

autor

Koziol S.

autor

Krzepilko A.

autor

Bartosz G.

autor

Bilinski T.

Bibliografia

• Benov L (2001) How superoxide radical damages the cell. Protoplasma 217: 33–36.
• Benov L, Fridovich I (1999) Why superoxide imposes an aromatic amino acid auxotrophy on Escherichia coli. The transketolase connection. J Biol Chem 274: 4202–4206.
• Benov L, Kredich NM, Fridovich I (1996) The mechanism of the auxotrophy for sulfur-containing amino acids imposed upon Escherichia coli by superoxide. J Biol Chem 271: 21037–21040.
• Bilinski T, Litwinska J (1987) On the ideas alternative to the theory of superoxide-mediated oxygen toxicity. Bull Pol Acad Sci 35: 25–31.
• Bilinski T, Krawiec Z, Liczmanski A, Litwinska J (1985) Is hydroxyl radical generated by the Fenton reaction in vivo? Biochem Biophys Res Commun 130: 533–539.
• Bilinski T, Litwinska J, Krawiec Z, Achremowicz B (1993) Physiological suppression of superoxide dismutase deficiency in yeast Saccharomyces cerevisiae. Acta Microbiol Pol 42: 101–104.
• Chang EC, Kosman DJ (1990) O2-dependent methionine auxotrophy in Cu,Zn superoxide dismutase-deficient mutants of Saccharomyces cerevisiae. J Bacteriol 172: 1840–1845.
• Chang EC, Crawford BF, Hong Z, Bilinski T, Kosman DJ (1991) Genetic and biochemical characterization of Cu,Zn superoxide dismutase mutants in Saccharomyces cerevisiae. J Biol Chem 266: 4417–4424.
• Garay-Arroyo A, Lledias F, Hansberg W, Covarrubias AA (2003) Cu,Zn-superoxide dismutase of Saccharomyces cerevisiae is required for resistance to hyperosmosis. FEBS Lett 539: 68–72.
• Hinze H, Holzer H (1986) Analysis of the energy metabolism after incubation of Saccharomyces cerevisiae with sulfite or nitrite. Arch Microbiol 145: 27–31.
• Kim S, Kirchman PA, Benguria A, Jazwinski SM (1999): Experimentation with the yeast model, In Methods in Aging Research, Yu BP, ed, pp. 191–213. CRC Press, Boca Raton, Boston, London, New York, Washington, D.C.
• Koziol S, Zagulski M, Bilinski T, Bartosz G (2005) Antioxidants protect the yeast Saccharomyces cerevisiae against hypertonic stress. Free Radic Res 39: 365–371.
• Krzepilko A, Swiecilo A, Wawryn J, Zadrag R, Koziol S, Bartosz G, Bilinski T (2004) Ascorbate restores lifespan of superoxide-dismutase deficient yeast. Free Radic Res 38: 1019–1024.
• Kuo CF, Mashino T, Fridovich I (1987) α,β-Dihydroxyisovalerate dehydratase. A superoxide-sensitive enzyme. J Biol Chem 262: 4724–4727.
• Lewinska A, Bilinski T, Bartosz G (2004) Limited effectiveness of antioxidants in the protection of yeast defective in antioxidant proteins. Free Radic Res 38: 1159–1165.
• Liu XF, Elashvili I, Gralla EB, Valentine JS, Lapinskas P, Culotta VC (1992) Yeast lacking superoxide dismutase. Isolation of genetic suppressors. J Biol Chem 267: 18298–18302.
• Schimz KL (1980) The effect of sulfite on the yeast Saccharomyces cerevisiae. Arch Microbiol 125: 89–95.
• Swiecilo A, Krawiec Z, Wawryn J, Bartosz G, Bilinski T (2000) Effect of stress on the life span of the yeast Saccharomyces cerevisiae. Acta Biochim Polon 47: 355–364.
• Wallace MA, Liou LL, Martins J, Clement MH, Bailey S, Longo VD, Valentine JS, Gralla EB (2004) Superoxide inhibits 4Fe-4S cluster enzymes involved in amino acid biosynthesis. Cross-compartment protection by CuZnsuperoxide dismutase. J Biol Chem 279: 32055–32062.
• Wawryn J, Krzepilko A, Myszka A, Bilinski T (1999) Deficiency in superoxide dismutases shortens life span of yeast cells. Acta Biochim Polon 46: 249–253.
• Zyracka E, Zadrag R, Koziol S, Krzepilko A, Bartosz G, Bilinski T (2005) Ascorbate abolishes auxotrophy caused by the lack of superoxide dismutase in Saccharomyces cerevisiae. Yeast can be a biosensor for antioxidants. J Biotechnol 115: 271–278.