Evaluation of real-time PCR method for rapid diagnosis of brucellosis with different clinical manifestations

• Autorzy: Surucuoglu S , El S. , Ural S. , Gazi H. , Kurutepe S. , Taskiran P. , Yurtsever S.G.
• Języki publikacji: EN

Abstrakty

EN

In this study, we tested the advantages of TaqMan real time PCR technique and compare it to conventional methods using serum samples from patients with different clinical forms of brucellosis. A total of 50 patients were included in the study. Blood culture using BACTEC 9240 system, Standard Wright's tube agglutination, and real time PCR methods were used. Control blood samples from 30 people with no history of brucellosis or exposure to Brucella spp. were examined too. Serological assay was positive for 49 patients (98%). Forty-four (88%) of the 50 patients had a positive PCR result, whereas Brucella spp were isolated from blood cultures of 18 patients (36%). STA test was all positive for focal brucellosis. Real time PCR test was positive in 9 patients with focal disease (90%), whereas blood culture was positive only in 4 patients (40%). The sensitivity, specificity, positive and negative predictive values of the real time PCR method were calculated as 88%, 100%, 100%, and 83%, respectively. Our results suggest that the high sensitivity and specificity of real time PCR method make it a useful tool for diagnosis of brucellosis with different clinical manifestations.

Słowa kluczowe

EN

blood sample control; blood culture; human disease; serological diagnosis; rapid diagnosis; patient; brucellosis; real-time polymerase chain reaction

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2009
• Tom: 58
• Numer: 1
• Opis fizyczny: p.15-19,ref.

Twórcy

autor

Surucuoglu S

• Celal Bayar University, School of Medicine, Manisa, Turkey

autor

El S.

autor

Ural S.

autor

Gazi H.

autor

Kurutepe S.

autor

Taskiran P.

autor

Yurtsever S.G.

Bibliografia

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