Overexpression of BimSs3, the novel isoform of Bim, can trigger cell apoptosis by inducing cytochrome c release from mitochondria

• Autorzy: Liu L. , Chen J. , Zhang J. , Ji C. , Zhang X. , Gu S. , Xie Y. , Mao Y.
• Języki publikacji: EN

Abstrakty

EN

Bim is defined as the pro-apoptotic BH3-only protein of the Bcl-2 family, which is a critical sensor and mediator in the mitochondrial-dependent apoptosis. In a previous work, we have cloned a novel transcript of Bim (GenBank accession number: AY305716) from the fetal brain cDNA, which is widely expressed in some carcinoma tissues and normal human tissues. According to the sequence analysis and the newly-defined nomenclature system of Bim isoforms (Adachi et al., 2005, Cell Death Differ 2: 192), we term it BimSs3 according to its characteristic structure. The subcellular location analysis indicated that the fused protein GFP-BimSs3 is distributed in the whole cell, mainly to the nucleus. Overexpression of BimSs3 in HEK293 cells causes apoptosis (28.16 ± 1.55%) compared to the negative control (5.44 ± 2.63%). It also causes cytochrome c release from the mitochondrial fraction to the cytosolic fraction during apoptosis. Western blotting assay indicates the molecular mass of GFP-BimSs3 is approximately 31.0 kDa (GFP: 27 kDa). Hence the open reading frame of BimSs3 may initiate at the second ATG and encodes a 36 amino-acid peptide with BH3 domain.

Słowa kluczowe

EN

Bim isoform; BimSs3 protein; cytochrome C; fetal brain; human normal tissue; immune system; sequence analysis; human carcinoma tissue; apoptosis; immunocyte apoptosis; Bcl-2 family

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2007
• Tom: 54
• Numer: 3
• Opis fizyczny: p.603-610,fig.,ref.

Twórcy

autor

Liu L.

• Fudan University, Shanghai, P.R.China

autor

Chen J.

autor

Zhang J.

autor

Ji C.

autor

Zhang X.

autor

Gu S.

autor

Xie Y.

autor

Mao Y.

Bibliografia

• Adachi M, Zhao X, Imai K (2005) Nomenclature of dynein light chain-linked BH3-only protein Bim isoforms. Cell Death Differ 2: 192–193.
• Adams JM, Cory S (1998) The Bcl-2 protein family: arbiters of cell survival. Science 5381: 1322–1326.
• Cereghetti GM, Scorrano L (2006) The many shapes of mitochondrial death. Oncogene 34: 4717–4724.
• Chen D, Zhou Q (2004) Caspase cleavage of BimEL triggers a positive feedback amplification of apoptotic signaling. Proc Natl Acad Sci 5: 1235–1240.
• Chen JZ, Ji CN, Gu SH, Li JX, Zhao EP, Huang Y, Huang L, Ying K, Xie Y, Mao YM (2004) Over-expression of Bim alpha3, a novel isoform of human Bim, result in cell apoptosis. Int J Biochem Cell Biol 8: 1554–1561.
• Chen L, Willis SN, Wei A, Smith BJ, Fletcher JI, Hinds MG, Colman PM, Day CL, Adams JM, Huang DC (2005) Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function. Mol Cell 3: 393–403.
• Cheng EH, Wei MC, Weiler S, Flavell RA, Mak TW, Lindsten T, Korsmeyer SJ (2001) BCL-2, BCL-X(L) sequester BH3 domain-only molecules preventing BAX- and BAK-mediated mitochondrial apoptosis. Mol Cell 3: 705–711.
• Cory S, Huang DC, Adams JM (2003) The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 53: 8590–8607.
• Daniel PT, Schulze-Osthoff K, Belka C, Guner D (2003) Guardians of cell death: the Bcl-2 family proteins. Essays Biochem 39: 73–88.
• Du C, Fang M, Li Y, Li L, Wang X (2000) Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 102: 33–42.
• Fadeel B, Orrenius S (2005) Apoptosis: a basic biological phenomenon with wide-ranging implications in human disease. J Intern Med 6: 479–517.
• Gross A, McDonnell JM, Korsmeyer SJ (1999) BCL-2 family members and the mitochondria in apoptosis. Genes Dev 15: 1899–1911.
• Heath-Engel HM, Shore GC (2006) Mitochondrial membrane dynamics, cristae remodeling and apoptosis. Biochim Biophys Acta 5: 549–560.
• Idogawa M, Adachi M, Minami T, Yasui H, Imai K (2003) Overexpression of BAD preferentially augments anoikis. Int J Cancer 2: 215–223.
• Jaruga E, Skierski J, Radziszewska E, Sikora E (2000) Proliferation and apoptosis of human T cells during replicative senescence — a critical approach Acta Biochim Polon 47:293-300
• Kuwana T, Bouchier-Hayes L, Chipuk JE, Bonzon C, Sullivan BA, Green DR, Newmeyer DD (2005) BH3 domains of BH3-only proteins differentially regulate Baxmediated mitochondrial membrane permeabilization both directly and indirectly. Mol Cell 4: 525–535.
• Letai A, Bassik MC, Walensky LD, Sorcinelli MD, Weiler S, Korsmeyer SJ (2002) Distinct BH3 domains either sensitize or activate mitochondrial apoptosis, serving as prototype cancer therapeutics. Cancer Cell 2: 183–192.
• Liu X, Kim CN, Yang J, Jemmerson R, Wang X (1996) Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell 86: 147–157.
• Liu JW, Chandra D, Tang SH, Chopra D, Tang DG (2002) Identification and characterization of Bim gamma, a novel proapoptotic BH3-only splice variant of Bim. Cancer Res 62: 2976–2981.
• Mami U, Miyashita T, Shikama Y, Tadokoro K, Yamada M (2001) Molecular cloning and characterization of six novel isoforms of human Bim, a member of the proapoptotic Bcl-2 family. FEBS Lett 509: 135–141.
• Marani M, Tenev T, Hancock D, Downward J, Lemoine NR (2002) Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis. Mol Cell Biol 22: 3577–3589.
• Moreau C, Cartron PF, Hunt A, Meflah K, Green DR, Evan G, Vallette FM, Juin P (2003) Minimal BH3 peptides promote cell death by antagonizing anti-apoptotic proteins. J Biol Chem 21: 19426–19435.
• Navratil JS, Liu CC, Ahearn JM (2006) Apoptosis and autoimmunity. Immunol Res 36: 3–12.
• O’Connor L, Strasser A, O’Reilly LA, Hausmann G, Adams JM, Cory S, Huang DC (1998) Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J 17: 384–395.
• Putcha GV, Harris CA, Moulder KL, Easton RM, Thompson CB, Johnson EM (2002) Intrinsic and extrinsic pathway signaling during neuronal apoptosis: lessons from the analysis of mutant mice. J Cell Biol 157: 441–453.
• Rathmell JC, Thompson CB (2002) Pathways of apoptosis in lymphocyte development, homeostasis, and disease. Cell 109: 97–107.
• Strasser A, Harris AW, Huang DC, Krammer PH, Cory S (1995) Bcl-2 and Fas/APO-1 regulate distinct pathways to lymphocyte apoptosis. EMBO J 24: 6136–6147.
• Straus SE, Sneller M, Lenardo MJ, Puck JM, Strober W (1999) An inherited disorder of lymphocyte apoptosis: the autoimmune lymphoproliferative syndrome. Ann Intern Med 130: 591–601.
• Wakabayashi T (1999) Structural changes of mitochondria related to apoptosis: swelling and megamitochondria formation. Acta Biochim Polon 46: 223–237.
• Wang X (2001) The expanding role of mitochondria in apoptosis. Genes Dev 15: 2922–2933.
• Wei MC, Zong WX, Cheng EH, Lindsten T, Panoutsakopoulou V, Ross AJ, Roth KA, MacGregor GR, Thompson CB, Korsmeyer SJ (2001) Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 5517: 624–626.
• Willis SN, Adams JM (2005) Life in the balance: how BH3–only proteins induce apoptosis. Curr Opin Cell Biol 6: 617–625.
• Zhang J, Chen J, Liu L, Ji C, Gu S, Ying K, Mao Y (2006) Different gene expression profiles of AD293 and HEK293 cell lines that show contrasting susceptibility to apoptosis induced by overexpression of Bim L. Acta Biochim Polon 53: 525–530.
• Zou H, Henzel WJ, Liu X, Lutschg A, Wang X (1997) Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90: 405–413.