Regulation of renal NAplus, Kplus-ATPase and ouabain-sensitive Hplus, Kplus-ATPase by the cyclic AMP-protein kinase A signal transduction pathway

• Autorzy: Beltowski A. , Marciniak A. , Wojcicka G. , Gorny D.
• Języki publikacji: EN

Abstrakty

EN

We investigated the effect of the cyclic AMP-protein kinase A (PKA) signalling path­way on renal Na+ ,K+ -ATPase and ouabain-sensitive H+ ,K+ -ATPase. Male Wistar rats were anaesthetized and catheter was inserted through the femoral artery into the ab­dominal aorta proximally to the renal arteries for infusion of the investigated sub­stances. Na+ ,K+ -ATPase activity was measured in the presence of Sch 28080 to block ouabain-sensitive H+ ,K+ -ATPase and improve specificity of the assay. Dibutyryl-cyclic AMP (db-cAMP) administered at a dose of 10-7 mol/kg per min and 10 -6mol/kg per min increased Na+ ,K+ -ATPase activity in the renal cortex by 34% and 42%, respec­tively, and decreased it in the renal medulla by 30% and 44%, respectively. db-cAMP infused at 10- mol/kg per min increased the activity of cortical ouabain-sensitive H+,K+ -ATPase by 33%, and medullary ouabain-sensitive H+,K+ -ATPase by 30%. All the effects of db-cAMP were abolished by a specific inhibitor of protein kinase A, KT 5720. The stimulatory effect on ouabain-sensitive H+ ,K+ -ATPase and on cortical Na+ ,K+ -ATPase was also abolished by brefeldin A which inhibits the insertion of pro­teins into the plasma membranes, whereas the inhibitory effect on medullary Na+ ,K+ -ATPase was partially attenuated by 17-octadecynoic acid, an inhibitor of cytochrome P450-dependent arachidonate metabolism. We conclude that the cAMP-PKA pathway stimulates Na+ ,K+ -ATPase in the renal cortex as well as ouabain-sensitive H+ ,K+ -ATPase in the cortex and medulla by a mechanism requiring insertion of proteins into the plasma membrane. In contrast, medullary Na+ ,K+ -ATPase is inhibited by cAMP through a mechanism involving cytochrome P450-dependent arachidonate metabolites.

Słowa kluczowe

EN

ATPase; signal transduction; signalling pathway; kidney; protein kinase A; AMP-protein kinase; cytochrome P450; sodium potassium

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 1
• Opis fizyczny: p.103-114,fig.

Twórcy

autor

Beltowski A.

• Medical University, Jaczewskiego 8, 20-090 Lublin, Poland

autor

Marciniak A.

autor

Wojcicka G.

autor

Gorny D.

Bibliografia

• Anderson RM, Cheng SXJ, Aperia A. (1998) Forskolin-induced down-regulation of Na+,K+-ATPase activity is not associated with internalization of the enzyme. Acta Physiol Scand.; 164: 39-46.
• Aperia A, Holtback U, Syren ML, Svensson LB, Fryckstedt J, Greengard P. (1994) Activation/deactivation of renal Na+,K+-ATPase: a final common pathway for regulation of natriuresis. FASEB J.; 8: 436-9.
• Beck JS, Marsolais M, Noel J, Breton S, Laprade R. (1995) Dibutyryl cyclic adenosine monophosphate stimulates the sodium pump in rabbit renal cortical tubules. Ren Physiol Biochem.; 18: 21-6.
• Beguin P, Beggah AT, Chibalin AV, Burgener-Kairuz P, Jaisser F, Mathews PM, Rossier BC, Cotecchia S, Geering K. (1994) Phosphorylation of the Na,K-ATPase alpha-subunit by protein kinase A and C in vitro and in intact cells. Identification of a novel motif for PKC-mediated phosphorylation. J Biol Chem.; 269: 24437-45.
• Beltowski J, Wojcicka G. (2002) Spectrophotometric method for the determination of renal ouabain-sensitive H+,K+- ATPase activity. Acta Biochim Polon.; 49: 515-27.
• Beltowski J, Marciniak A, Wojcicka G, Gorny D. (2002) The opposite effects of cyclic AMP-protein kinase A signal transdcution pathway on renal cortical and medullary Na+,K+-ATPase activity. J Physiol Pharmacol.; 53: 211-31.
• Bertorello AM, Ridge KM, Chibalin AV, Katz AI, Sznajder JI. (1999) Isoproterenol increases Na+,K+&-ATPase activity by membrane insertion of alpha-subunits in lung alveolar cells. Am J Physiol.; 276: L20-7.
• Breton S, Beck JS, Laprade R. (1994) cAMP stimulates proximal convoluted tubule Na+-K+-ATPase activity. Am J Physiol.; 266: F400-10.
• Buffin-Meyer B, Younes-Ibrahim M, Barlet-Bas C, Cheval L, Marsy S, Doucet A. (1997) Kdepletion modifies the properties of Sch-28080- sensitive K-ATPase in rat collecting duct. Am J Physiol.; 272: F124-31.
• Carranza ML, Feraille E, Kiroytscheva M, Rousselot M, Favre H. (1996). Stimulation of ouabain-sensitive 86Rb+ uptake and Na+,K+-ATPase alpha-subunit phosphorylation by a cAMP-dependent signalling pathway in intact cells from rat kidney cortex. FEBSLett.; 396: 309-14.
• Carranza ML, Rousselot M, Chibalin AV, Bertorello AM, Favre H, Feraille E. (1998) Protein kinase A induces recruitment of active Na+,K+-ATPase units to the plasma membrane of rat proximal convoluted tubule cells. J Physiol. ; 511: 235-43.
• Charlton JA, Baylis PH. (1990) Stimulation of rat renal medullary Na+/K+-ATPase by arginine vasopressin is mediated by the V2 receptor. J Endocrinol.; 127: 213-6.
• Cheng XJ, Fisone G, Aizman O, Aizman R, Levenson R, Greengard P, Aperia A. (1997) PKA-mediated phosphorylation and inhibition of Na+-K+-ATPase in response to beta-adrenergic hormone. Am J Physiol.; 273: C893-901.
• Cheng SXJ, Aizman O, Nairn AC, Greengard P, Aperia A. (1999) [Ca2+] determines the effects of protein kinases A and C on activity of rat renal Na+-K+-ATPase. J Physiol. ; 518: 37-46.
• Cornelius F, Logvinenko N. (1996) Functional regulation of reconstituted Na,K-ATPase by protein kinase A phosphorylation. FEBS Lett; 380: 277-80.
• Doucet A. (1997) H+,K+-ATPase in the kidney: localization and function in the nephron. Exp Nephrol.; 5: 271-6.
• Feraille E, Doucet A (2001). Sodium-potassium- adenosinetriphosphatase-dependent sodium transport in the kidney: Hormonal control. Physiol Rev.; 81: 345-418.
• Feschenko MS, Sweadner KJ. (1994) Conformation-dependent phosphorylation of Na,K-ATPase by protein kinase A and protein kinase C. J Biol Chem.; 269: 30436-44.
• Feschenko MS, Stevenson E, Sweadner KJ. (2000) Interaction of protein kinase C and cAMP-dependent pathways in the phosphorylation of the Na,K-ATPase. J Biol Chem.; 275: 34693-700.
• Fisone G, Cheng SX, Nairn AC, Czernik AJ, Hemmings HC Jr, Hoog JO, Bertorello AM, Kaiser R, Bergman T, Jornvall H. (1994) Identification of the phosphorylation site for cAMP-dependent protein kinase on Na+,K+-ATPase and effects of site-directed mutagenesis. J Biol Chem.; 269: 9368-73.
• Fryckstedt J, Aperia A. (1992) Sodium-dependent regulation of sodium, potassium-adenosine-tri-phosphatase (Na+, K+- ATPase) activity in medullary thick ascending limb of Henle segments. Effect of cyclic-adenosine-monophosphate guanosine-nucleotide-binding-protein activity and arginine vasopressin. Acta Physiol Scand.; 144: 185-90.
• Gonin S, Deschenes G, Roger F, Bens M, Martin PY, Carpentier JL, Vandewalle A, Doucet A, Feraille E. (2001) Cyclic AMP increases cell surface expression of functional Na,K-ATPase units in mammalian cortical collecting duct principal cells. Mol Biol Cell.; 12: 255-64.
• Higuchi E, Nishi A, Higashi H, Ito Y, Kato H. (2000) Phosphorylation of protein phosphatase-1 inhibitors, inhibitor-1 and DARPP-32, in renal medulla. Eur J Pharmacol.; 408: 107-16.
• Hussain T, Abdul-Wahab R, Lokhandwala MF. (1997) Bromocriptine stimulates Na+, K+-ATPase in renal proximal tubules via the cAMP pathway. Eur J Pharmacol.; 321: 259-63.
• Kiroytscheva M, Cheval L, Carranza ML, Martin PY, Favre H, Doucet A, Feraille E. (1999) Effect of cAMP on the activity and the phosphorylation of Na+,K+-ATPase in rat thick ascending limb of Henle. Kidney Int. ; 55: 1819-31.
• Kone BC, Higham SC. (1998) A novel N-terminal splice variant of the rat H+,K+-ATPase 2 subunit. J Biol Chem.; 273: 2543-52.
• Kurihara K, Nakanishi N, Ueda T. (2000) Regulation of Na+-K+-ATPase by cAMP-dependent protein kinase anchored on membrane via its anchoring protein. Am J Physiol Cell Physiol.; 279: C1516-27.
• Laroche-Joubert N, Marsy S, Doucet A. (2000) Cellular origin and hormonal regulation of K+-ATPase activities sensitive to Sch-28080 in rat collecting duct. Am J Physiol Renal Physiol.; 279: F1053-9.
• Laroche-Joubert N, Marsy S, Michelet S, Imbert-Teboul M, Doucet A. (2002) Protein kinase A-independent activation of ERK and H,K-ATPase by cAMP in native kidney cells. J Biol Chem.; 277: 18598-604.
• Lowry OH, Rosebrough NI, Farr AL, Randall RJ. (1951) Protein measurement with the Folin phenol reagent. J Biol Chem.; 193: 265-75.
• Meister B, Fryckstedt J, Schalling M, Cortes R, Hokfelt T, Aperia A, Hemmings HC Jr, Nairn AC, Ehrlich M, Greengard P. (1989) Dopamine- and cAMP-regulated phosphoprotein (DARPP-32) and dopamine DA1 agonist-sensitive Na+-K+- ATPase in renal tubule cells. Proc Natl Acad Sci US A.; 86: 8068-72.
• Nowicki S, Chen SL, Aizman O, Cheng XJ, Li D, Nowicki C, Nairn A, Greengard P, Aperia A. (1997) 20- Hydroxyeicosatetraenoic acid (20- HETE) activates protein kinase C. Role in regulation of rat renal Na+,K+-ATPase. J Clin Invest.; 99: 1224-30.
• Satoh T, Cohen HT, Katz AI. (1992) Intracellular signaling in the regulation of renal Na-K-ATPase. I. Role of cyclic AMP and phospholipase A2. J Clin Invest.; 89: 1496-500.
• Satoh T, Cohen HT, Katz AI. (1993a) Different mechanisms of renal Na-K-ATPase regulation by protein kinases in proximal and distal nephron. Am J Physiol.; 265: F399-405.
• Satoh T, Cohen HT, Katz AI. (1993b) Intracellular signaling in the regulation of renal Na-K-ATPase. II. Role of eicosanoids. J Clin Invest.; 91: 409-15.
• Schwartzman M, Ferreri NR, Carrol MA, Songu-Mize E, McGiff JC. (1985) Renal cytochrome P450-related arachidonate metabolite inhibits (Na++K+)ATPase. Nature.; 314: 620-2.
• Sweadner KJ, Feschenko MS. (2001) Predicted location and limited accessibility of protein kinase A phosphorylation site on Na,K-ATPase. Am J Physiol Cell Physiol.; 280: C1017-26.
• Younes-Ibrahim M, Barlet-Bas C, Buffin-Meyer B, Cheval L, Rajerison R, Doucet A. (1995) Ouabain-sensitive and - insensitive K-ATPases in rat nephron: effect of K depletion. Am J Physiol.; 268: F1141-7.