Eosinophil - epithelial cell interaction augments cysteinyl leukotrienes synthesis

• Autorzy: Jawien J. , Chlopicki S. , Olszanecki R. , Lorkowska B. , Gryglewski R.J.
• Języki publikacji: EN

Abstrakty

EN

Eosinophils accumulation in the airways and sustained eosinophil-derived cysteinyl leukotrienes production represent key elements of the inflammatory response seen in asthma.However,it is not known whether activated epithelial cells influence cysteinyl leukotrienes production by eosinophils from healthy valunteers.The aim of the present study was therefore to analyse the effects of interactions between non-atopic eosinophils and epithelial cells on cysteinyl leukotrienes production in vitro .We measured cysteinyl leukotrienes released by phorbol 12-myristate 13-acetate (PMA)–activated human eosinophils or epithelial cells (human bronchial epithelial cell line -BEAS-2B)cultured alone or together.While activated BEAS-2B cells barely formed leukotrienes (1.39 pg/ml ± 0.2))(n=32),activated eosinophils produced considerable amount of them (62.25 pg/ml ± 10.29))(n=32).Interestingly,when activated eosinophils and epithelial cells were co-incubated,production of cysteinyl leukotrienes increased substantially (571.1 pg/ml ± 80.9))(n=32).Thus,eosinophil-epithelial cell interactions,when occur,are associated with increased biosythesis of cysteinyl leukotrienes.

Słowa kluczowe

EN

asthma; cell-cell interaction; epithelial cell; eosinophil; cysteinyl leukotriene synthesis

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2002
• Tom: 53
• Numer: 1
• Opis fizyczny: p.127-132,fig.

Twórcy

autor

Jawien J.

• Jagiellonian University School of Medicine, Grzegorzecka Str.16, 31-531 Krakow, Poland

autor

Chlopicki S.

autor

Olszanecki R.

autor

Lorkowska B.

autor

Gryglewski R.J.