Partial deglycosylation of alpha subunit modifies sturgeon gonadotropin function

• Autorzy: Zenkevics H. , Vose V. , Vosekalne I. , Bucena A.
• Języki publikacji: EN

Abstrakty

EN

Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (alphaGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dgalphaGTH + betaGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed that deglycosylation led to changes in the secondary structure of dgalphaGTH and in the alpha-beta recombinant. The sugar moiety of sturgeon alphaGTH is suggested to play an important role in maintaining the biological function of the hormone dimer molecule.

Słowa kluczowe

EN

Acipenser gueldenstaedti; deglycosylation; hormonal function; immunoreactivity; sturgeon; carbohydrate content; hormone; gonadotrophic hormone

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2000
• Tom: 47
• Numer: 3
• Opis fizyczny: p.815-819,fig.

Twórcy

autor

Zenkevics H.

• Latvian University, Salaspils, Latvia

autor

Vose V.

autor

Vosekalne I.

autor

Bucena A.

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