Effect of reduced glutathione [GSH] on activity of lysosomal system in subcellular fractions of mouse kidney

• Autorzy: Sliwa-Jozwik A , Jozwik A. , Fronczyk W. , Guszkiewicz A. , Kolataj A.
• Języki publikacji: EN

Abstrakty

EN

Investigated was the effect of intraperitoneal injections (twice daily for seven consecutive days) of reduced glutathione (GSH) on activity of selected lysosomal hydrolases (alanine aminopeptidase, leucine aminopeptidase, cathepsins D and L, acid phosphatase and N-acetyl-β-D-glucosaminidase) in lysosomal, microsomal and cytosol fractions of mouse kidney. Most notable effect of exogenous GSH on the activity of the enzymes considered was observed in lysosomal, while the least in microsomal fraction. After a series of GSH injections the activity of enzymes increased significantly mainly In lysosomal fraction. It is assumed that the increase in activity of lysosomal enzymes following the GSH injections results from a physiological response of renal cells to this antioxidant.

PL

Celem pracy było zbadanie, czy dootrzewnowe iniekcje zredukowanego glutationu (GSH) wpływają na aktywność enzymów lizosomowych w lizosomowej, mikrosomowej i cytozolowej frakcji komórkowej nerki myszy. Zwierzęta utrzymywano w warunkach standardowych z pełnym dostępem do paszy i wody.Myszy grupy doświadczalnej (n=10) otrzymały w ciągu 7 dni 14 dootrzewnowych iniekcji GSH, po 100 μg GSH/g masy ciała w 0,9% NaCl. Myszom grupy kontrolnej (n=10) podawano wyłącznie 0,9% NaCl.We frakcjach komórkowych nerki oznaczono aminopeptydazę alanylową, aminopeptydazę leucynową,katepsynę D i L, kwaśną fosfatazę i N-acetylo-β-D-glucosaminidazę. Najwyższą aktywność enzymów po iniekcjach GSH zaobserwowano we frakcji lizosomowej. Przypuszcza się, że wzrost aktywności badanych enzymów lizosomowych jest wynikiem adaptacyjnych zmian we frakcjach komórkowych, które wynikają z antyoksydacyjnych właściwości GSH.

Słowa kluczowe

EN

hydrolase; mouse; mice; lysosome; kidney; glutathione; subcellular fraction

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2004
• Tom: 22
• Numer: 2
• Opis fizyczny: p.237-245,fig.,ref.

Twórcy

autor

Sliwa-Jozwik A

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Jozwik A.

autor

Fronczyk W.

autor

Guszkiewicz A.

autor

Kolataj A.

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