Differences in editing of mitochondrial nad3 transcripts from CMS and fertile carrots

• Autorzy: Rurek M. , Szklarczyk M. , Adamczyk N. , Michalik B. , Augustyniak H.
• Języki publikacji: EN

Abstrakty

EN

A high level of the nucleotide sequence conservation was found for mitochondrial nad3 gene of carrot. Three silent nucleotide substitutions differentiate nad3 open reading frames from cytoplasmic male sterile and male fertile carrots. All these differ­ences are preserved on the RNA level. Partial and silent editing also distinguished both carrots. Three of the C to U conversions were specific to the fertile line. In the two examined carrot lines editing did not affect the mode of alteration of encoded amino acids.

Słowa kluczowe

EN

nad3 gene; RNA; carrot; Daucus carota; RNA level; hexadecyltrimethylammonium bromide; polymerase chain reaction; amino acid; cytoplasmic male sterility

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 3
• Opis fizyczny: p.711-717,fig.

Twórcy

autor

Rurek M.

• Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland

autor

Szklarczyk M.

autor

Adamczyk N.

autor

Michalik B.

autor

Augustyniak H.

Bibliografia

• 1.Sarria, R., Lyznik, A., Vallejos, C.E. & Mackenzie, S.A. (1998) A cytoplasmic male sterility-associated mitochondrial peptide in common bean is post-translationally regulated. Plant Cell 10, 1217-1228.
• 2.Nakajima, Y., Yamamoto, T., Muranaka, T. & Oeda, K. (2001) A novel or/B-related gene of carrot mitochondrial genomes that is associated with homeotic cytoplasmic male sterility (CMS). Plant Mol. Biol. 46, 99-107.
• 3.Stein, M. & Nothnagel, T. (1995) Some remarks on carrot breeding (Daucus carota sativus Hoffm.). Plant Breed. 114, 1-11.
• 4.Kitagawa, J., Posluszny, U., Gerrath, J.M. & Wolyn, D.J. (1994) Developmental and morphological analyses of homeotic cytoplasmic male sterile and fertile carrot flowers. Sex Plant Reprod. 7, 41-50.
• 5.Bowes, C.E. & Wolyn, D.J. (1998) Phylogenetic relationships among fertile and petaloid male-sterile accessions of carrot, Daucus carota L. Theor. Appl. Genet. 96, 928-932.
• 6.Scheike, R.G.E., Brennicke, A. & Mehring- Lemper, M.G. (1992) Unique patterns of mitochondrial genes, transcripts and proteins in different male-sterile cytoplasms of Daucus carota. Theor. Appl. Genet. 83, 419-427.
• 7.Szklarczyk, M. (1997) Unique features of carrot mtDNAs from CMS and maintainer lines. J. Appl. Genet. 38A, 42-56.
• 8.Szklarczyk, M., Oczkowski, M., Augustyniak, H., Boerner, T., Linke, B. & Michalik, B. (2000) Organisation and expression of mitochondrial atp9 genes from CMS and fertile carrots. Theor. Appl. Genet. 100, 263-270.
• 9.Wilson, R.K. & Hanson, M.R. (1996) Preferential RNA editing at specific sites within transcripts of two plant mitochondrial genes does not depend on transcriptional context or nuclear genotype. Curr. Genet. 30, 502-508.
• 10.Hanson, M.R., Wilson, R.K., Bentolila, S., Kohler, R.H. & Chen, H.C. (1999) Mitochondrial gene organization and expression in petunia male fertile and sterile plants. J. Hered. 90, 362-368.
• 11.Rurek, M., Oczkowski, M. & Augustyniak, H. (1998) Conservation of the structure and organization of lupin mitochondrial nad3 and rps12 genes. Acta Biochim. Polon. 45, 695- 699.
• 12.Gawal, N.J. & Jarret, R.L. (1991) A modified CTAB DNA extraction procedure for Musca and Ipomea. Plant Mol. Biol. Rep. 9, 262-266.
• 13.Steinborn, R., Weihe, A. & Boerner, T. (1992) Mitochondrial genome diversity within a cultivar of Daucus carota (ssp. sativus) revealed by restriction analysis of single plants. Plant Breed. 109, 75-77.
• 14.Chomczynski, P. & Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 2, 156-159.
• 15.Gelvin, S.B., Schilperoort, R.A. & Verma, M. (1991) Plant Molecular Biology Manual. Kluwer Academic Publ., Dordrecht, The Netherlands.
• 16.Sanger, F., Nickeln, S. & Coulson, A.R. (1979) DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74, 5463-5467.
• 17.Perotta, G., Regina, T.M.R., Ceci, L.R. & Quagliariello, C. (1996) Conservation of the organization of the mitochondrial nad3 and rps12 genes in evolutionarily distant angiosperms. Mol. Gen. Genet. 251, 326-337.
• 18.Maier, R.M., Zeltz, P., Kossel, H., Bonnard, G., Gualberto, J. & Grienenberger, J.-M. (1996) RNA editing in plant mitochondria and chloroplasts. Plant Mol. Biol. 32, 343-365.
• 19.Lu, B. & Hanson, M.R. (1996) Fully edited and partially edited nad9 transcripts differ in size and both are associated with polysomes in potato mitochondria. Nucleic Acids Res. 24, 1369-1374.
• 20.Gualberto, J.M., Bonnard, G., Lamattina, L. & Grienenberger, J.-M. (1991) Expression of the wheat mitochondrial nad3-rps12 transcription unit: correlation between editing and mRNA maturation. Plant Cell 3, 1109-1120.
• 21.Schuster, W., Wissinger, B., Unseld, M. & Brennicke, A. (1990) Transcripts of the NADH-dehydrogenase subunit 3 gene are differentially edited in Oenothera mitochondria. EMBO J. 9, 263-269.
• 22.Itani, K. & Handa, H. (1998) Rapeseed mitochondrial ccb206, a gene involved in cytochrome c biogenesis, is co-transcribed with the nad3 and rps12 genes; Organization, transcription, and RNA editing of the nad3/rps12/ccb206locus. Curr. Genet. 34, 318-325.
• 23.Howad, W. & Kempken, F. (1997) Sequence analysis and transcript processing of the mitochondrial nad3-rps12 genes from Sorghum bicolor. Plant Sci. 129, 65-68.
• 24.Suzuki, T., Kazama, S., Hirai, A., Akihama, T. & Kadowaki, K. (1991) The rice mitochondrial nad3 gene has an extended reading frame at its 5' end: Nucleotide sequence analysis of rice trnS, nad3, and rps12. Curr. Genet. 20, 331- 337.
• 25.Grosskopf, D. & Mulligan, R.M. (1996) Developmental- and tissue-specifity of RNA editing in mitochondria of suspension-cultured maize cells and seedlings. Curr. Genet. 29, 556-563.
• 26.Dias, S.M.G., Siquera, S.F., Lejeune, B. & de Souza, A.P. (2000) Identification and characterization of the trnS/pseudo-tRNA/nad3/ rps12 cluster from Coix lacryma-jobi L.: Organization, transcription and RNA editing. Plant Sci. 158, 97-105.
• 27.Karpinska, B., Karpinski, S. & Hallgren, J.-E. (1995) The genes encoding subunit 3 of NADH dehydrogenase and ribosomal protein S12 are co-transcribed and edited in Pinus sylvestris (L.) mitochondria. Curr. Genet. 28, 423-428.