Identification and tissue distribution of a novel rat glucocorticoid receptor splice variant

• Autorzy: Ju H. , Wang X. , Liu Z. , Liu P. , Zhao Y. , Xiong R. , Zhou Y. , Chen X.
• Języki publikacji: EN

Abstrakty

EN

 Glucocorticoid receptor (GR) is a steroid hormone receptor that has been shown to play important roles in diverse cellular and physiological processes. More and more evidence has revealed that the effects of glucocorticoids are mediated by the glucocorticoid receptor through genomic or nongenomic mechanisms. A growing number of glucocorticoid receptor splice variants have been identified in human tissues, but few are known in rat tissues. In this work, a novel rGR cDNA, called rGRβ, was cloned from Sprague Dawlay (SD) rat liver. Sequence analysis revealed that the rGRβ mRNA was 39 base pairs (bp) shorter than the rGR mRNA reported earlier. The deleted segment is located in exon 1 and encodes 13 repeated glutamine residues. Both the rGR and rGRβ mRNAs were quantitated by Northern blot hybridization using non-homologous glucocorticoid cDNA probes. Results showed that the rGR and rGRβ mRNAs were most abundant in the lung, the least abundant in the heart, and there were more rGR and rGRβ mRNAs in the kidney than in the liver. The identification of rGRβ may contribute to the understanding of the genomic or nongenomic effects of glucocorticoids.

Słowa kluczowe

EN

receptor isoform; human tissue; physiological process; tissue distribution; non-genomic effect; splice variant; glucocorticoid; rat; glucocorticoid receptor; rat tissue

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 1
• Opis fizyczny: p.109-113,fig.,ref.

Twórcy

autor

Ju H.

• Third Military Medical University, Chongqing, China, 400042

autor

Wang X.

autor

Liu Z.

autor

Liu P.

autor

Zhao Y.

autor

Xiong R.

autor

Zhou Y.

autor

Chen X.

Bibliografia

• Bamberger CM, Bamberger AM, de Castro M, Chrousos GP (1995) Glucocorticoid receptor beta, a potential endogenous inhibitor of glucocorticoid action in humans. J Clin Invest 95: 2435-2441.
• Barnes PJ, Adcock IM (2003) How do corticosteroids work in asthma? Ann Intern Med 139: 359-370.
• Beck CA, Estes PA, Bona BJ, Muro-Cacho CA, Nordeen SK, Edwards DP (1993) The steroid antagonist RU486 exerts different effects on the glucocorticoid and progesterone receptors. Endocrinology 133: 728-740.
• Busse WW, Lenfant C, Lemanske RF Jr (2002) Asthma guidelines: a changing paradigm to improve asthma care. J Allergy Clin Immunol 110: 703-705.
• Franchimont D (2004) Overview of the actions of glucocorticoids on the immune response: a good model to characterize new pathways of immunosuppression for new treatment strategies. Ann NY Acad Sci 1024: 124-137.
• Gearing KL, Gustafsson JA, Okret S (1993) Heterogeneity in the polyglutamine tract of the glucocorticoid receptor from different rat strains. Nucleic Acids Res 21: 2014.
• Hafezi-Moghadam A, Simoncini T, Yang Z, Limbourg FP, Plumier JC, Rebsamen MC, Hsieh CM, Chui DS, Thomas KL, Prorock AJ (2002) Acute cardiovascular protective effects of corticosteroids are mediated by non-transcriptional activation of endothelial nitric oxide synthase. Nat Med 8: 473-479.
• Iniguez-Lluhi JA, Lou DY, Yamamoto KR (1997) Three amino acid substitutions selectively disrupt the activation but not the repression function of the glucocorticoid receptor N terminus. J Biol Chem 272: 4149-4156.
• Ito K, Chung KF, Adcock IM (2006) Update on glucocorticoid action and resistance. J Allergy Clin Immunol 117: 522-543.
• Kalinyak JE, Dorin RI, Hoffman AR, Perlman AJ (1987) Tissue specific regulation of glucocorticoid receptor mRNA by dexamethasone. J Biol Chem 262: 10441-10444.
• Keller-Wood ME, Dallman MF (1984) Corticosteroid inhibition of ACTH secretion. Endocr Rev 5: 1-24.
• Kitraki E, Karandrea D, Kittas C (1999) Long-lasting effects of stress on glucocorticoid receptor gene expression in the rat brain. Neuroendocrinology 69: 331-338.
• Lavery DN, McEwan IJ (2005) Structure and function of steroid receptor AF1 transactivation domains: induction of active conformations. Biochem J 391: 449-464.
• Levy FO, Ree AH, Eikvar L, Govindan MV, Jahnsen T, Hansson V (1989) Glucocorticoid receptors and glucocorticoid effects in rat Sertoli cells. Endocrinology 124: 430-436.
• Lowenberg M, Tuynman J, Bilderbeek J, Gaber T, Buttgereit F, van Deventer S, Peppelenbosch M, Hommes D (2005) Rapid immunosuppressive effects of glucocorticoids mediated through Lck and Fyn. Blood 106: 1703-1710.
• Mao J, Regelson W, Kalimi M (1992) Molecular mechanism of RU 486 action: a review. Mol Cell Biochem 109: 1-8.
• Miesfeld R, Okret S, Wikstrom AC, Wrange O, Gustafsson JA, Yamamoto KR (1984) Characterization of a steroid hormone receptor gene and mRNA in wild-type and mutant cells. Nature 312: 779-781.
• Miesfeld R, Rusconi S, Godowski PJ, Maler BA, Okret S, Wikstrom AC, Gustafsson JA, Yamamoto KR (1986) Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA. Cell 46: 389-399.
• Mitchell PJ, Tjian R (1989) Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245: 371-378.
• Oakley RH, Webster JC, Sar M, Parker CR Jr, Cidlowski JA (1997) Expression and subcellular distribution of the β-isoform of the human glucocorticoid receptor. Endocrinology 138: 5028-5038.
• Pfeiffer A, Barden N (1988) Glucocorticoid receptor gene expression in rat pituitary gland intermediate lobe following ovariectomy. Mol Cell Endocrinol 55: 115-120.
• Pratt WB, Galigniana MD, Morishima Y, Murphy PJ (2004) Role of molecular chaperones in steroid receptor action. Essays Biochem 40: 41-58.
• Pujols L, Mullol J, Picado C (2007) Alpha and beta glucocorticoid receptors: relevance in airway diseases. Curr Allergy Asthma Rep 7: 93-99.
• Pujols L, Mullol J, Torrego A, Picado C (2004) Glucocorticoid receptors in human airways. Allergy 59: 1042-1052.
• Rhen T, Cidlowski JA (2005) Antiinflammatory action of glucocorticoids — new mechanisms for old drugs. N Engl J Med 353: 1711-1723.
• Scadding GK (2001) Corticosteroids in the treatment of pediatric allergic rhinitis. J Allergy Clin Immunol 108: S59-64.
• Yamamoto KR (1985) Steroid receptor regulated transcription of specific genes and gene networks. Annu Rev Genet 19: 209-252.